Matching Items (156)
Description
The City of Phoenix Street Transportation Department partnered with the Rob and Melani Walton Sustainability Solutions Service at Arizona State University (ASU) and researchers from various ASU schools to evaluate the effectiveness, performance, and community perception of the new pavement coating. The data collection and analysis occurred across multiple neighborhoods and at varying times across days and/or months over the course of one year (July 15, 2020–July 14, 2021), allowing the team to study the impacts of the surface treatment under various weather conditions.
ContributorsMiddel, Ariane (Author) / Vanos, Jennifer K. (Author) / Hondula, David M. (Author) / Kaloush, Kamil (Author) / Sailor, David (Author) / Medina, Jose R. (Jose Roberto) (Author) / Schneider, Florian (Author) / Ortiz, Johny Cordova (Author) / Campbell, Bill (Author) / Epel, Erin (Contributor) / Rice, Brendan (Contributor) / Garcia, Ruth (Contributor) / Phoenix (Ariz.). Street Transportation Department (Contributor) / City of Phoenix (Funder) / Industrial Development Authority of the County of Maricopa (Funder) / Julie Ann Wrigley Global Institute of Sustainability (Funder) / Arizona State University. Healthy Urban Environments Program (Contributor)
Created2021-09
Description
Food waste is one of the most significant food system inefficiencies with environmental, financial, and social consequences. This waste, which occurs more at the consumer stage in high income countries, is often attributed to consumers’ behavior. While behavior is a contributing factor, the role of other contextual factors in influencing this behavior has not been systematically analyzed. Understanding contextual drivers of consumer food waste behavior is important, as behavior sits in a matrix of technology, infrastructures, institutions and social structure. Hence designing effective interventions will require a systems perceptive of the problem. In paper 1, I used Socio-ecological framing to understand how personal, interpersonal, socio-cultural, built, and institutional environments contribute to food waste at the consumer stage. In paper 2, I explored the perception of stakeholders in Phoenix on the effectiveness and feasibility of possible interventions that could be used to tackle consumer food waste. In paper 3, I examined the impact of knowledge and awareness of the environmental consequence of food waste in terms of embedded water and energy on the cognitive factors responsible for consumer food waste behavior. Across these three papers, I have identified three findings. First, the most influential factor responsible for consumer food waste is meal planning, as many decisions about food management depend on it. However, there are many contextual factors that discourage meal planning. Other factors identified include the wide gap between food producers and consumers, the low price of food, and marketing strategies used by retailers to encourage food purchases. Systems level interventions will be required to address these drivers that provide an enabling environment for behavioral change. Second stakeholders in the city overwhelmingly support and agree that education will be the most effective and feasible intervention to address consumer food waste, 3) there is need to carefully craft education materials to inform consumers about other resources, such as water and energy, embedded in food waste to stimulate a personal norm that motivates change in behavior. In this study, I emphasize the need to understand the root causes of consumer food waste and exploration of systems level interventions, in combination with education and information interventions that are being commonly used.
ContributorsOpejin, Adenike Kafayat (Author) / Aggarwal, Rimjhim (Thesis advisor) / White, Dave (Thesis advisor) / Garcia, Margret (Committee member) / Merrigan, Kathleen (Committee member) / Arizona State University (Publisher)
Created2022
Description
Producing, transforming, distributing, and consuming food requires a multitude of actors, from the microbes in the soil to the truck drivers, from the salesperson to the bacterial life that supports digestion. Yet, the global food system – far from being neutral – unequally provides and extracts resources around the globe to serve and protect the needs of some, while excluding and/or oppressing others and producing trauma in the process. Drawing on feminist scholarship and permaculture research – two fields that discuss the importance of care but only rarely work together – and using social science methods, I explore how to integrate care into food systems, and what are the outcomes of such an integration. I first bring together the voices of 35 everyday experts from Cuba, France, and the United States (Arizona) and perspectives from ethics of care, creation care, indigenous scholars, and permaculture specialists, and I use grounded theory to develop a definition of care in food systems context, and a conceptual map of care that identifies motives for caring, caring practices and their results. I then discuss how caring practices enhance food systems’ adaptive capacity and resilience. Next, I study the relationship between a subset of the identified caring practices – what is recognized as “Earth care” – and their effect on well-being in general, and Food Well-Being more specifically, using three case studies from Arizona based on: (1) interviews of school teachers, (2) interviews of sustainable farmers, (3) a survey with 96 gardeners. There, I also discuss how policies and cultural transformations can better support the integration of Earth care practices in food systems. Then, I examine how urban food autonomy movements are grassroots examples of integration of care in food systems, and how through their care practices – Earth care, “People care” and “Fair share” – they can serve as a catalyst for social change and contribute to the achievement of the United Nations Sustainable Development Goals. Lastly, I conclude with recommendations to strengthen a culture of care in food systems, as well as limitations to my research, and future research directions.
ContributorsGiraud, Esteve Gaelle (Author) / Aggarwal, Rimjhim (Thesis advisor) / Cloutier, Scott (Thesis advisor) / Samuelson, Hava (Committee member) / Chhetri, Netra (Committee member) / Arizona State University (Publisher)
Created2022
Description
Computational models have long been used to describe and predict the outcome of complex immunological processes. The dissertation work described here centers on the construction of multiscale computational immunology models that derives biological insights at the population, systems, and atomistic levels. First, SARS-CoV-2 mortality is investigated through the lens of the predicted robustness of CD8+ T cell responses in 23 different populations. The robustness of CD8+ T cell responses in a given population was modeled by predicting the efficiency of endemic MHC-I protein variants to present peptides derived from SARS-CoV-2 proteins to circulating T cells. To accomplish this task, an algorithm, called EnsembleMHC, was developed to predict viral peptides with a high probability of being recognized by CD T cells. It was discovered that there was significant variation in the efficiency of different MHC-I protein variants to present SARS-CoV-2 derived peptides, and countries enriched with variants with high presentation efficiency had significantly lower mortality rates. Second, a biophysics-based MHC-I peptide prediction algorithm was developed. The MHC-I protein is the most polymorphic protein in the human genome with polymorphisms in the peptide binding causing striking changes in the amino acid compositions, or binding motifs, of peptide species capable of stable binding. A deep learning model, coined HLA-Inception, was trained to predict peptide binding using only biophysical properties, namely electrostatic potential. HLA-Inception was shown to be extremely accurate and efficient at predicting peptide binding motifs and was used to determine the peptide binding motifs of 5,821 MHC-I protein variants. Finally, the impact of stalk glycosylations on NL63 protein dynamics was investigated. Previous data has shown that coronavirus crown glycans play an important role in immune evasion and receptor binding, however, little is known about the role of the stalk glycans. Through the integration of computational biology, experimental data, and physics-based simulations, the stalk glycans were shown to heavily influence the bending angle of spike protein, with a particular emphasis on the glycan at position 1242. Further investigation revealed that removal of the N1242 glycan significantly reduced infectivity, highlighting a new potential therapeutic target. Overall, these investigations and associated innovations in integrative modeling.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis advisor) / Singharoy, Abhishek (Thesis advisor) / Woodbury, Neal (Committee member) / Sulc, Petr (Committee member) / Arizona State University (Publisher)
Created2022
DescriptionThis thesis explores what factors have hindered the effectiveness of international aid in promoting sustainable development—an approach that focuses on not just immediate responses, but long-term changes that promote the well-being of current and future generations in relation to environmental degradation, social equity, and economic growth—in Haiti.
ContributorsLee, Rachel (Author) / Aggarwal, Rimjhim (Thesis director) / Mueller, Valerie (Committee member) / Sivak, Henry (Committee member) / Barrett, The Honors College (Contributor) / Watts College of Public Service & Community Solut (Contributor) / School of Sustainability (Contributor) / School of Politics and Global Studies (Contributor)
Created2022-05
Description
This work focuses on a novel approach to combine electrical current with cyanobacterial technology, called microbial electrophotosynthesis (MEPS). It involves using genetically modified PSII-less Synechocystis PCC 6803 cells to avoid photoinhibition, a problem that hinders green energy. In the work, a cathodic electron delivery system is employed for growth and synthesis. Photoinhibition leads to the dissipation energy and lower yield, and is a major obstacle to preventing green energy from competing with fossil fuels. However, the urgent need for alternative energy sources is driven by soaring energy consumption and rising atmospheric carbon dioxide levels. When developed, MEPS can contribute to a carbon capture technology while helping with energy demands. It is thought that if PSII electron flux can be replaced with an alternative source photosynthesis could be enhanced for more effective production. MEPS has the potential to address these challenges by serving as a carbon capture technology while meeting energy demands. The idea is to replace PSII electron flux with an alternative source, which can be enhanced for higher yields in light intensities not tolerated with PSII. This research specifically focuses on creating the initiation of electron flux between the cathode and the MEPS cells while controlling and measuring the system in real time.
The successful proof-of-concept work shows that MEPS can indeed generate high-light-dependent current at intensities up to 2050 µmol photons m^‒2 s^‒1, delivering 113 µmol electrons h^‒1 mg-chl^‒1. The results were further developed to characterize redox tuning for electron delivery of flux to the photosynthetic electron transport chain and redox-based kinetic analysis to model the limitations of the MEPS system.
ContributorsLewis, Christine Michelle (Author) / Torres, César I (Thesis advisor) / Fromme, Petra (Thesis advisor) / Woodbury, Neal (Committee member) / Hayes, Mark (Committee member) / Arizona State University (Publisher)
Created2023
Description
Adaptation to climate change is a core sustainability challenge across the Global South. Development and government organizations conceptualize and govern climate adaptation by creating national and sub-national action plans and implementing projects. This dissertation confronts the inherent tensions that arise when formal planned adaptation interventions encounter the complex, often messy realities of the implementation context. In doing so, this research examines how planned adaptation—with its incentives, provisioned resources, prescribed behaviors, and expectations of commitment from target beneficiaries —interacts with individuals and communities already balancing diverse risks while pursuing their livelihood aspirations. Two broad questions guide this dissertation: 1) how is adaptation envisioned by planners and practitioners? and, 2) how do project beneficiaries engage with, and experience planned adaptation interventions? The research employs an exploratory and inductive qualitative research design. Using Foucault’s lens of governmentality, this research utilises document analysis to examine how the first wave of Indian adaptation projects envision goals, conceptualize problems, delineate roles, and frame expectations of intended beneficiaries. Next, using a case study of an adaptation project implemented in Uttarakhand, India, the study examines the motivations and associated trade-offs behind the engagement and disengagement of the intended beneficiaries: smallholder farmers. Insights from gender-differentiated focus group discussions guide this analysis. Both inquiries are supplemented with findings from semi-structured interviews with Indian adaptation experts and project implementers. The analysis finds that: 1) project reports construct identities of the climate vulnerable beneficiary, implicitly assigning roles and transferring responsibilities for sustaining adaptation efforts beyond project timelines, 2) project participants are not default beneficiaries, but instead exercise agency in decision-making by either opting-in or opting-out of planned initiatives, and 3) the implicit and explicit costs of engaging in planned adaptation interventions are substantial, encompassing significant contributions of time, physical labor, and active participation during and post the project period. This dissertation challenges existing notions of whom planned adaptation serves, and to what end, offering new insights into its design and effectiveness. Furthermore, this research suggests that for planned adaptation to be sustainable, a concerted effort to align with evolving needs, aspirations and livelihood shifts of those on the frontlines of climate change is essential.
ContributorsYogya, Yamini (Author) / Eakin, Hallie (Thesis advisor) / Aggarwal, Rimjhim (Committee member) / York, Abigail (Committee member) / Agrawal, Arun (Committee member) / Arizona State University (Publisher)
Created2024
Description
Given increasing frequency and intensity of extreme weather events and disparities in socio-economic conditions, managing flood risks has become ever more challenging. Building a community flood resilience becomes an essential strategy to reduce flood risks and achieve sustainable development. However, enhancing community flood resilience presents numerous obstacles and potential downsides. To address such a challenge, this dissertation investigates the interactions among natural, built environment, and actor systems using a case study of flooding in Jakarta, Indonesia, focusing on the Ciliwung river watershed. The study first develops a conceptual framework for community flood resilience by integrating concepts from disaster, flood, and community resilience research to represent how water, structural elements, and actors are connected and can contribute to hazards and vulnerabilities. Building on the framework, the study utilizes a mixed-methods approach, combining stakeholder interviews with network and heatmap analysis to identify the roles and interconnections of relevant actors in Jakarta’s flooding. Next, the study employs a hybrid of agent-based and system dynamics modeling to explore the complex interactions among the watershed’s physical and actor systems. The model evaluates how the level of collaboration among government agencies affects flood management interventions and community resilience under various scenarios concerning weather patterns and land-use change.
The research provides an exploratory tool and a strategic guide for enhancing community flood resilience. The stakeholder analysis reveals the complexity of stakeholder relationships and challenges such as imbalance in resource allocation, authority overlap, and low engagement. These insights highlight the need for trust-building, community involvement, and strategic alignment in flood management efforts. Systems modeling to derive policy recommendations bridges the gap between theoretical frameworks and governance challenges, offering empirical evidence of the critical role of governmental collaboration in reducing exposure to floods. The systems modeling results suggest that under conditions of uncertainties, enhancing community flood resilience requires a coordinated approach among stakeholders.
ContributorsSulistyo, Sinta Rahmawidya (Author) / Agusdinata, Datu Buyung (Thesis advisor) / Aggarwal, Rimjhim (Committee member) / Brundiers, Katja (Committee member) / Arizona State University (Publisher)
Created2024
Description
The fundamental photophysics of fluorescent probes must be understood when the probes are used in biological applications. The photophysics of BODIPY dyes inside polymeric micelles and rhodamine dyes covalently linked to proteins were studied. Hydrophobic boron-dipyrromethene (BODIPY) dyes were noncovalently encapsulated inside polymeric micelles. Absorbance and fluorescence measurements were employed to study the photophysics of these BODIPY dyes in the micellar environments. Amphiphilic polymers with a hydrophobic character and low Critical Micelle Concentration (CMC) protected BODIPYS from the aqueous environment. Moderate dye loading conditions did not result in ground-state dimerization, and only fluorescence lifetimes and brightnesses were affected. However, amphiphilic polymers with a hydrophilic character and high CMC did not protect the BODIPYS from the aqueous environment with concomitant ground-state dimerization and quenching of the fluorescence intensity, lifetime, and brightnesses even at low dye loading conditions. At the doubly-labeled interfaces of Escherichia coli (E. coli) DNA processivity β clamps, the interchromophric interactions of four rhodamine dyes were studied: tetramethylrhodamine (TMR), TMR C6, Alexa Fluor 488, and Alexa Fluor 546. Absorbance and fluorescence measurements were performed on doubly-labeled β clamps with singly-labeled β clamps and free dyes as controls. The absorbance measurements revealed that both TMR and TMR C6 readily formed H-dimers (static quenching) at the doubly-labeled interfaces of the β clamps. However, the TMR with a longer linker (TMR C6) also displayed a degree of dynamic quenching. For Alexa Fluor 546 and Alexa Fluor 488, there were no clear signs of dimerization in the absorbance scans. However, the fluorescence properties (fluorescence intensity, lifetime, and anisotropy) of the Alexa Fluor dyes significantly changed when three methodologies were employed to disrupt the doubly-labeled interfaces: 1) the addition of sodium dodecyl sulfate (SDS) detergent to denature the proteins, 2) the addition of clamp loader (γ complex) to open one of the two interfaces, and 3) the use of subunit exchange to decrease the number of dyes per interface. These fluorescence measurements indicated that for the Alexa Fluor dyes, other interchromophoric interactions were present such as dynamic quenching and homo-Förster Resonance Energy Transfer (homo-FRET).
ContributorsDonaphon, Bryan Matthew (Author) / Levitus, Marcia (Thesis advisor) / Van Horn, Wade (Committee member) / Woodbury, Neal (Committee member) / Arizona State University (Publisher)
Created2018
Description
The highly predictable structural and thermodynamic behavior of deoxynucleic acid (DNA) and ribonucleic acid (RNA) have made them versatile tools for creating artificial nanostructures over broad range. Moreover, DNA and RNA are able to interact with biological ligand as either synthetic aptamers or natural components, conferring direct biological functions to the nucleic acid devices. The applications of nucleic acids greatly relies on the bio-reactivity and specificity when applied to highly complexed biological systems.
This dissertation aims to 1) develop new strategy to identify high affinity nucleic acid aptamers against biological ligand; and 2) explore highly orthogonal RNA riboregulators in vivo for constructing multi-input gene circuits with NOT logic. With the aid of a DNA nanoscaffold, pairs of hetero-bivalent aptamers for human alpha thrombin were identified with ultra-high binding affinity in femtomolar range with displaying potent biological modulations for the enzyme activity. The newly identified bivalent aptamers enriched the aptamer tool box for future therapeutic applications in hemostasis, and also the strategy can be potentially developed for other target molecules. Secondly, by employing a three-way junction structure in the riboregulator structure through de-novo design, we identified a family of high-performance RNA-sensing translational repressors that down-regulates gene translation in response to cognate RNAs with remarkable dynamic range and orthogonality. Harnessing the 3WJ repressors as modular parts, we integrate them into biological circuits that execute universal NAND and NOR logic with up to four independent RNA inputs in Escherichia coli.
This dissertation aims to 1) develop new strategy to identify high affinity nucleic acid aptamers against biological ligand; and 2) explore highly orthogonal RNA riboregulators in vivo for constructing multi-input gene circuits with NOT logic. With the aid of a DNA nanoscaffold, pairs of hetero-bivalent aptamers for human alpha thrombin were identified with ultra-high binding affinity in femtomolar range with displaying potent biological modulations for the enzyme activity. The newly identified bivalent aptamers enriched the aptamer tool box for future therapeutic applications in hemostasis, and also the strategy can be potentially developed for other target molecules. Secondly, by employing a three-way junction structure in the riboregulator structure through de-novo design, we identified a family of high-performance RNA-sensing translational repressors that down-regulates gene translation in response to cognate RNAs with remarkable dynamic range and orthogonality. Harnessing the 3WJ repressors as modular parts, we integrate them into biological circuits that execute universal NAND and NOR logic with up to four independent RNA inputs in Escherichia coli.
ContributorsZhou, Yu (Ph.D.) (Author) / Yan, Hao (Thesis advisor) / Green, Alexander (Thesis advisor) / Woodbury, Neal (Committee member) / Ros, Alexandra (Committee member) / Arizona State University (Publisher)
Created2019