Matching Items (2)
Filtering by
- All Subjects: Electric Fields
- Creators: Ghrilanda, Giovanna
Description
Bioparticles comprise a diverse amount of materials ubiquitously present in nature. From proteins to aerosolized biological debris, bioparticles have important roles spanning from regulating cellular functions to possibly influencing global climate. Understanding their structures, functions, and properties provides the necessary tools to expand our fundamental knowledge of biological systems and exploit them for useful applications. In order to contribute to this efforts, the work presented in this dissertation focuses on the study of electrokinetic properties of liposomes and novel applications of bioaerosol analysis. Using immobilized lipid vesicles under the influence of modest (less than 100 V/cm) electric fields, a novel strategy for bionanotubule fabrication with superior throughput and simplicity was developed. Fluorescence and bright field microscopy was used to describe the formation of these bilayer-bound cylindrical structures, which have been previously identified in nature (playing crucial roles in intercellular communication) and made synthetically by direct mechanical manipulation of membranes. In the biological context, the results of this work suggest that mechanical electrostatic interaction may play a role in the shape and function of individual biological membranes and networks of membrane-bound structures. A second project involving liposomes focused on membrane potential measurements in vesicles containing trans-membrane pH gradients. These types of gradients consist of differential charge states in the lipid bilayer leaflets, which have been shown to greatly influence the efficacy of drug targeting and the treatment of diseases such as cancer. Here, these systems are qualitatively and quantitatively assessed by using voltage-sensitive membrane dyes and fluorescence spectroscopy. Bioaerosol studies involved exploring the feasibility of a fingerprinting technology based on current understanding of cellular debris in aerosols and arguments regarding sampling, sensitivity, separations and detection schemes of these debris. Aerosolized particles of cellular material and proteins emitted by humans, animals and plants can be considered information-rich packets that carry biochemical information specific to the living organisms present in the collection settings. These materials could potentially be exploited for identification purposes. Preliminary studies evaluated protein concentration trends in both indoor and outdoor locations. Results indicated that concentrations correlate to certain conditions of the collection environment (e.g. extent of human presence), supporting the idea that bioaerosol fingerprinting is possible.
ContributorsCastillo Gutiérrez, Josemar Andreina (Author) / Hayes, Mark A. (Thesis advisor) / Herckes, Pierre (Committee member) / Ghrilanda, Giovanna (Committee member) / Arizona State University (Publisher)
Created2011
Description
Electric utilities are exploring new technologies to cope up with the in-crease in electricity demand and power transfer capabilities of transmission lines. Compact transmission lines and high phase order systems are few of the techniques which enhance the power transfer capability of transmission lines without requiring any additional right-of-way. This research work investigates the impact of compacting high voltage transmission lines and high phase order systems on the surface electric field of composite insulators, a key factor deciding service performance of insulators. The electric field analysis was done using COULOMB 9.0, a 3D software package which uses a numerical analysis technique based on Boundary Element Method (BEM). 3D models of various types of standard transmission towers used for 230 kV, 345 kV and 500 kV level were modeled with different insulators con-figurations and number of circuits. Standard tower configuration models were compacted by reducing the clearance from live parts in steps of 10%. It was found that the standard tower configuration can be compacted to 30% without violating the minimum safety clearance mandated by NESC standards. The study shows that surface electric field on insulators for few of the compact structures exceeded the maximum allowable limit even if corona rings were installed. As a part of this study, a Gaussian process model based optimization pro-gram was developed to find the optimum corona ring dimensions to limit the electric field within stipulated values. The optimization program provides the dimen-sions of corona ring, its placement from the high voltage end for a given dry arc length of insulator and system voltage. JMP, a statistical computer package and AMPL, a computer language widely used form optimization was used for optimi-zation program. The results obtained from optimization program validated the industrial standards.
ContributorsMohan, Nihal (Author) / Gorur, Ravi S. (Thesis advisor) / Heydt, Gerald T. (Committee member) / Vittal, Vijay (Committee member) / Arizona State University (Publisher)
Created2012