Matching Items (120)
Description
Oxidative aging is an important factor in the long term performance of asphalt pavements. Oxidation and the associated stiffening can lead to cracking, which in turn can lead to the functional and structural failure of the pavement system. Therefore, a greater understanding of the nature of oxidative aging in asphalt pavements can potentially be of great importance in estimating the performance of a pavement before it is constructed. Of particular interest are the effects of aging on asphalt rubber pavements, due to the fact that, as a newer technology, few asphalt rubber pavement sections have been evaluated for their full service life. This study endeavors to shed some light on this topic. This study includes three experimental programs on the aging of asphalt rubber binders and mixtures. The first phase addresses aging in asphalt rubber binders and their virgin bases. The binders were subjected to various aging conditions and then tested for viscosity. The change in viscosity was analyzed and it was found that asphalt rubber binders exhibited less long term aging. The second phase looks at aging in a laboratory environment, including both a comparison of accelerated oxidative aging techniques and aging effects that occur during long term storage. Dynamic modulus was used as a tool to assess the aging of the tested materials. It was found that aging materials in a compacted state is ideal, while aging in a loose state is unrealistic. Results not only showed a clear distinction in aged versus unaged material but also showed that the effects of aging on AR mixes is highly dependant on temperature; lower temperatures induce relatively minor stiffening while higher temperatures promote much more significant aging effects. The third experimental program is a field study that builds upon a previous study of pavement test sections. Field pavement samples were taken and tested after being in service for 7 years and tested for dynamic modulus and beam fatigue. As with the laboratory aging, the dynamic modulus samples show less stiffening at low temperatures and more at higher temperatures. Beam fatigue testing showed not only stiffening but also a brittle behavior.
ContributorsReed, Jordan (Author) / Kaloush, Kamil (Thesis advisor) / Mamlouk, Michael (Committee member) / Zapata, Claudia (Committee member) / Arizona State University (Publisher)
Created2010
Description
In the middle of the 20th century in the United States, transportation and infrastructure development became a priority on the national agenda, instigating the development of mathematical models that would predict transportation network performance. Approximately 40 years later, transportation planning models again became a national priority, this time instigating the development of highly disaggregate activity-based traffic models called microsimulations. These models predict the travel on a network at the level of the individual decision-maker, but do so with a large computational complexity and processing time requirement. The vast resources and steep learning curve required to integrate microsimulation models into the general transportation plan have deterred planning agencies from incorporating these tools. By researching the stochastic variability in the results of a microsimulation model with varying random number seeds, this paper evaluates the number of simulation trials necessary, and therefore the computational effort, for a planning agency to reach stable model outcomes. The microsimulation tool used to complete this research is the Transportation Analysis and Simulation System (TRANSIMS). The requirements for initiating a TRANSIMS simulation are described in the paper. Two analysis corridors are chosen in the Metropolitan Phoenix Area, and the roadway performance characteristics volume, vehicle-miles of travel, and vehicle-hours of travel are examined in each corridor under both congested and uncongested conditions. Both congested and uncongested simulations are completed in twenty trials, each with a unique random number seed. Performance measures are averaged for each trial, providing a distribution of average performance measures with which to test the stability of the system. The results of this research show that the variability in outcomes increases with increasing congestion. Although twenty trials are sufficient to achieve stable solutions for the uncongested state, convergence in the congested state is not achieved. These results indicate that a highly congested urban environment requires more than twenty simulation runs for each tested scenario before reaching a solution that can be assumed to be stable. The computational effort needed for this type of analysis is something that transportation planning agencies should take into consideration before beginning a traffic microsimulation program.
ContributorsZiems, Sarah Elia (Author) / Pendyala, Ram M. (Thesis advisor) / Ahn, Soyoung (Committee member) / Kaloush, Kamil (Committee member) / Arizona State University (Publisher)
Created2010
Description
Rapid developments are occurring in the arena of activity-based microsimulation models. Advances in computational power, econometric methodologies and data collection have all contributed to the development of microsimulation tools for planning applications. There has also been interest in modeling child daily activity-travel patterns and their influence on those of adults in the household using activity-based microsimulation tools. It is conceivable that most of the children are largely dependent on adults for their activity engagement and travel needs and hence would have considerable influence on the activity-travel schedules of adult members in the household. In this context, a detailed comparison of various activity-travel characteristics of adults in households with and without children is made using the National Household Travel Survey (NHTS) data. The analysis is used to quantify and decipher the nature of the impact of activities of children on the daily activity-travel patterns of adults. It is found that adults in households with children make a significantly higher proportion of high occupancy vehicle (HOV) trips and lower proportion of single occupancy vehicle (SOV) trips when compared to those in households without children. They also engage in more serve passenger activities and fewer personal business, shopping and social activities. A framework for modeling activities and travel of dependent children is proposed. The framework consists of six sub-models to simulate the choice of going to school/pre-school on a travel day, the dependency status of the child, the activity type, the destination, the activity duration, and the joint activity engagement with an accompanying adult. Econometric formulations such as binary probit and multinomial logit are used to obtain behaviorally intuitive models that predict children's activity skeletons. The model framework is tested using a 5% sample of a synthetic population of children for Maricopa County, Arizona and the resulting patterns are validated against those found in NHTS data. Microsimulation of these dependencies of children can be used to constrain the adult daily activity schedules. The deployment of this framework prior to the simulation of adult non-mandatory activities is expected to significantly enhance the representation of the interactions between children and adults in activity-based microsimulation models.
ContributorsSana, Bhargava (Author) / Pendyala, Ram M. (Thesis advisor) / Ahn, Soyoung (Committee member) / Kaloush, Kamil (Committee member) / Arizona State University (Publisher)
Created2010
Description
Dielectrophoresis has been shown in the recent past to successfully separate bioparticles of very subtle differences at high resolutions using biophysical forces. In this study, we test the biophysical differences of methicillin resistant and susceptible Staph. aureus that are known to have very similar genomes by using a modified gradient insulator-based dielectrophoresis device (g-iDEP). MRSA is commonly seen in hospitals and is the leading killer of infectious bacteria, claiming the lives of around 10,000 people annually. G-iDEP improves many capabilities within the DEP field including sample size, cost, ease of use and analysis time. This is a promising foundation to creating a more clinically optimized diagnostic tool for both separation and detection of bacteria in the healthcare field. The capture on-set potential for fluorescently tagged MRSA (801 ± 34V) is higher than fluorescently tagged MSSA (610 ± 32V), resulting in a higher electrokinetic to dielectrophoretic mobility ratio for MRSA. Since the strains have proven to be genomically similar through sequencing, it is reasonable to attribute this significant biophysical difference to the added PBP2a enzyme in MRSA. These results are consistent with other bacterial studied within in this device and have proven to be reproducible.
ContributorsSmithers, Jared (Author) / Hayes, Mark (Thesis director) / Woodbury, Neal (Committee member) / School of Criminology and Criminal Justice (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Diabesity is a global epidemic affecting millions worldwide. Diabesity is the term given to the link between obesity and Type II diabetes. It is estimated that ~90% of patients diagnosed with Type II diabetes are overweight or have struggled with excess body fat in the past. Type II diabetes is characterized by insulin resistance which is an impaired response of the body to insulin that leads to high blood glucose levels. Adipose tissue, previously thought of as an inert tissue, is now recognized as a major endocrine organ with an important role in the body's immune response and the development of chronic inflammation. It is speculated that adipose tissue inflammation is a major contributor to insulin resistance particular to Type II diabetes. This literature review explores the popular therapeutic targets and marketed drugs for the treatment of Type II diabetes and their role in decreasing adipose tissue inflammation. rAGE is currently in pre-clinical studies as a possible target to combat adipose tissue inflammation due to its relation to insulin resistance. Metformin and Pioglitazone are two drugs already being marketed that use unique chemical pathways to increase the production of insulin and/or decrease blood glucose levels. Sulfonylureas is one of the first FDA approved drugs used in the treatment of Type II diabetes, however, it has been discredited due to its life-threatening side effects. Bariatric surgery is a form of invasive surgery to rid the body of excess fat and has shown to normalize blood glucose levels. These treatments are all secondary to lifestyle changes, such as diet and exercise which can help halt the progression of Type II diabetes patients.
ContributorsRobles, Alondra Maria (Author) / Woodbury, Neal (Thesis director) / Redding, Kevin (Committee member) / Allen, James (Committee member) / Hendrickson, Kirstin (Committee member) / Sanford School of Social and Family Dynamics (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The Heliobacterial Reaction Center (HbRC) is the simplest Type I Reaction Center (RC) known today. However, upon illumination it has been found to produce menaquinol, and this has led to experiments investigating the function of this reduction scheme. The goal of the experiment was to investigate the mechanisms of menaquinol production through the use of Photosystem II (PSII) herbicides that are known to inhibit the QB quinone site in Type II RCs. Seven herbicides were chosen, and out of all of them terbuthylazine showed the greatest effect on the RC in isolated membranes when Transient Absorption Spectroscopy was used. In addition, terbuthylazine decreased menaquinone reduction to menaquinol by ~72%, slightly more than the reported effect of teburtryn (68%)1. In addition, terbuthylazine significantly impacted growth of whole cells under high light more than terbutryn.
ContributorsOdeh, Ahmad Osameh (Author) / Redding, Kevin (Thesis director) / Woodbury, Neal (Committee member) / Allen, James (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Asphalt pavements deteriorate over time and are subjected to various distresses like rutting, fatigue cracking, stripping, raveling, etc. In this study, an experiment to indirectly assess aggregate stripping was completed in order to evaluate the effect of type of binder, and aging on the binder-aggregate bond under dry conditioning. The asphalts used in the study are commonly used in the state of Arizona, which included both non-polymer modified and polymer modified asphalts. The phenomenon of stripping was simulated using the Bitumen Bond Strength Test (BBS) and evaluated for Arizona binders. The BBS test is a simple test that measures the "pull-off" tensile strength of the bond between asphalt and the aggregate. Polymer modified binders were found to have lower pull-off strength in comparison to the non-modified or neat binder which were found to possess greater pull-off strength, but lower elasticity, causing the failure to become brittle and spontaneous. However, when aged binder was used, the bond strength expectedly reduced for non-polymer modified asphalts but surprisingly increased for polymer modified asphalts. Both un-aged neat and polymer modified binders were observed to have a cohesive failure whereas only the aged polymer modified binders failed in cohesion. The aged non-polymer modified binders were seen to have an adhesive failure.
ContributorsPonce, Esai Jonathon (Author) / Kaloush, Kamil (Thesis director) / Gundla, Akshay (Committee member) / Civil, Environmental and Sustainable Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Predicting the binding sites of proteins has historically relied on the determination of protein structural data. However, the ability to utilize binding data obtained from a simple assay and computationally make the same predictions using only sequence information would be more efficient, both in time and resources. The purpose of this study was to evaluate the effectiveness of an algorithm developed to predict regions of high-binding on proteins as it applies to determining the regions of interaction between binding partners. This approach was applied to tumor necrosis factor alpha (TNFα), its receptor TNFR2, programmed cell death protein-1 (PD-1), and one of its ligand PD-L1. The algorithms applied accurately predicted the binding region between TNFα and TNFR2 in which the interacting residues are sequential on TNFα, however failed to predict discontinuous regions of binding as accurately. The interface of PD-1 and PD-L1 contained continuous residues interacting with each other, however this region was predicted to bind weaker than the regions on the external portions of the molecules. Limitations of this approach include use of a linear search window (resulting in inability to predict discontinuous binding residues), and the use of proteins with unnaturally exposed regions, in the case of PD-1 and PD-L1 (resulting in observed interactions which would not occur normally). However, this method was overall very effective in utilizing the available information to make accurate predictions. The use of the microarray to obtain binding information and a computer algorithm to analyze is a versatile tool capable of being adapted to refine accuracy.
ContributorsBrooks, Meilia Catherine (Author) / Woodbury, Neal (Thesis director) / Diehnelt, Chris (Committee member) / Ghirlanda, Giovanna (Committee member) / Department of Psychology (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The free-base tetra-tolyl-porphyrin and the corresponding cobalt and iron porphyrin complexes were synthesized and characterized to show that this class of compound can be promising, tunable catalysts for carbon dioxide reduction. During cyclic voltammetry experiments, the iron porphyrin showed an on-set of ‘catalytic current’ at an earlier potential than the cobalt porphyrin’s in organic solutions gassed with carbon dioxide. The cobalt porphyrin yielded larger catalytic currents, but at the same potential as the electrode. This difference, along with the significant changes in the porphyrin’s electronic, optical and redox properties, showed that its capabilities for carbon dioxide reduction can be controlled by metal ions, allotting it unique opportunities for applications in solar fuels catalysis and photochemical reactions.
ContributorsSkibo, Edward Kim (Author) / Moore, Gary (Thesis director) / Woodbury, Neal (Committee member) / School of Molecular Sciences (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
ABSTRACT Peptide microarrays may prove to be a powerful tool for proteomics research and clinical diagnosis applications. Fodor et al. and Maurer et al. have shown proof-of-concept methods of light- and electrochemically-directed peptide microarray fabrication on glass and semiconductor microchips respectively. In this work, peptide microarray fabrication based on the abovementioned techniques were optimized. In addition, MALDI mass spectrometry based peptide synthesis characterization on semiconductor microchips was developed and novel applications of a CombiMatrix (CBMX) platform for electrochemically controlled synthesis were explored. We have investigated performance of 2-(2-nitrophenyl)propoxycarbonyl (NPPOC) derivatives as photo-labile protecting group. Specifically, influence of substituents on 4 and 5 positions of phenyl ring of NPPOC group on the rate of photolysis and the yield of the amine was investigated. The results indicated that substituents capable of forming a π-network with the nitro group enhanced the rate of photolysis and yield. Once such properly substituted NPPOC groups were used, the rate of photolysis/yield depended on the nature of protected amino group indicating that a different chemical step during the photo-cleavage process became the rate limiting step. We also focused on electrochemically-directed parallel synthesis of high-density peptide microarrays using the CBMX technology referred to above which uses electrochemically generated acids to perform patterned chemistry. Several issues related to peptide synthesis on the CBMX platform were studied and optimized, with emphasis placed on the reactions of electro-generated acids during the deprotection step of peptide synthesis. We have developed a MALDI mass spectrometry based method to determine the chemical composition of microarray synthesis, directly on the feature. This method utilizes non-diffusional chemical cleavage from the surface, thereby making the chemical characterization of high-density microarray features simple, accurate, and amenable to high-throughput. CBMX Corp. has developed a microarray reader which is based on electro-chemical detection of redox chemical species. Several parameters of the instrument were studied and optimized and novel redox applications of peptide microarrays on CBMX platform were also investigated using the instrument. These include (i) a search of metal binding catalytic peptides to reduce overpotential associated with water oxidation reaction and (ii) an immobilization of peptide microarrays using electro-polymerized polypyrrole.
ContributorsKumar, Pallav (Author) / Woodbury, Neal (Thesis advisor) / Allen, James (Committee member) / Johnston, Stephen (Committee member) / Arizona State University (Publisher)
Created2013