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  1. KEEP
  2. Theses and Dissertations
  3. Barrett, The Honors College Thesis/Creative Project Collection
  4. Use of microRNA to Determine the Age of Forensically Relevant Blow Fly Pupae
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Use of microRNA to Determine the Age of Forensically Relevant Blow Fly Pupae

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Description

Medicolegal forensic entomology is the study of insects to aid with legal investigations (Gemmellaro, 2017). Insect evidence can be used to provide information such as the post-mortem interval (PMI). Blow flies are especially useful as these insects are primary colonizers, quickly arriving at a corpse (Malainey & Anderson, 2020). The age of blow flies found at a scene is used to calculate the PMI. Blow fly age can be estimated using weather data as these insects are poikilothermic (Okpara, 2018). Morphological analysis also can be used to estimate age; however, it is more difficult with pupal samples as the pupae exterior does not change significantly as development progresses (Bala & Sharma, 2016). Gene regulation analysis can estimate the age of samples. MicroRNAs are short noncoding RNA that regulate gene expression (Cannell et al., 2008). Here, we aim to catalog miRNAs expressed during the development of three forensically relevant blow fly species preserved in several storage conditions. Results demonstrated that various miRNA sequences were differentially expressed across pupation. Expression of miR92b increased during mid pupation, aga-miR-92b expression increased during early pupation, and bantam, miR957, and dana-bantam-RA expression increased during late pupation. These results suggest that microRNA can be used to estimate the age of pupal samples as miRNA expression changes throughout pupation. Future work could develop a statistical model to accurately determine age using miRNA expression patterns.

Date Created
2021-05
Contributors
  • Herrera-Quiroz, Demian David (Author)
  • Parrott, Jonathan (Thesis director)
  • Weidner, Lauren (Committee member)
  • School of Mathematical and Natural Sciences (Contributor, Contributor)
  • Barrett, The Honors College (Contributor)
Topical Subject
  • Forensic Entomology
  • blow flies
  • microRNA
Resource Type
Text
Extent
21 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Barrett, The Honors College Thesis/Creative Project Collection
Series
Academic Year 2020-2021
Handle
https://hdl.handle.net/2286/R.I.63612
Level of coding
minimal
Cataloging Standards
asu1
System Created
  • 2021-04-17 12:26:57
System Modified
  • 2021-08-11 04:09:57
  •     
  • 1 year 9 months ago
Additional Formats
  • OAI Dublin Core
  • MODS XML

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