Theses and dissertations

This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students. 

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Exploring the Paternal Voice's Impact on the Fetus

Description

Peer-reviewed literature on the effects of sound and music on babies ranging from the prenatal period to the postnatal period, called the perinatal period moving forward, is vast. Substantial research has been directed towards the neural connections they make with sound, musical therapy, audio learning, and much more. The primary…

Peer-reviewed literature on the effects of sound and music on babies ranging from the prenatal period to the postnatal period, called the perinatal period moving forward, is vast. Substantial research has been directed towards the neural connections they make with sound, musical therapy, audio learning, and much more. The primary focus of this thesis was to review the current literature on how the father's voice affects the fetus during the perinatal period. During the preliminary research of the topic, the process faced an immediate problem as this concept had very little substantial sources. The number of relevant articles or documents with the term “father” in the title can be counted on one hand. In some cases, research that explored the father’s voice did so only as a supporting statistic or comparison to the main goal of exploring the mother’s voice. The mother’s voice has been prioritized as a research topic because of the immediate physical connection between the mother and the child throughout the entire pregnancy. The third semester of pregnancy is often a period of study since what the mother senses, says or does will somehow translate into an experience for the child’s developing brain. On the other hand, the father’s voice has been considered an environmental sound, a position justified by some researchers due to phenomena such as differing levels of fetal attention and observed “preference” of a newborn. The scarcity of research regarding the father’s voice, or the voice of a non-parental male, can be explained in several ways. These include the father’s potential absence due to work, inherited ideologies and biases against active parenting for the father, parental roles, and their unwillingness to utilize infant-directed speech and singing for psychological reasons, during the perinatal period, compared to that of the mother. Researching ways to deepen the relationship between the father and baby during the perinatal period, along with developing new biotechnologies for different kinds of evaluative tests, will help advance the subject matter of how the father’s voice impacts a baby to a larger scale.
ContributorsNguyen, Justin Dinh Huy (Author) / Norton, Kay (Thesis director) / Lefler, Scott (Committee member) / School of Music, Dance and Theatre (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Purification and Characterization of TRI 05 I13S M6I

Description
2,2’ bipyridine (Bpy) can form metal complexes with divalent metals in the form of [M(Bpy-ala)¬3]+2 where M is any divalent metal. These [M(Bpy-ala)¬3]+2 complexes can have very interesting photochemical and redox potentials that can be useful in more complex systems. The use of (2,2′-bipyridin-5yl)alanine (Bpy-ala) as a Noncanonical Amino Acid…

2,2’ bipyridine (Bpy) can form metal complexes with divalent metals in the form of [M(Bpy-ala)¬3]+2 where M is any divalent metal. These [M(Bpy-ala)¬3]+2 complexes can have very interesting photochemical and redox potentials that can be useful in more complex systems. The use of (2,2′-bipyridin-5yl)alanine (Bpy-ala) as a Noncanonical Amino Acid (NCAA) has allowed Bpy to be incorporated into an amino acid sequence which can now function in a protein scaffold. Previous studies have utilized that power of Bpy-ala to design a protein that can assemble a homotrimeric protein complex in the presence of a divalent metal. However, the issue with this design was that when the homotrimer was formed and the divalent was removed, the protein complex would not dissemble indicating that it was not metal dependent. Point mutations were made to disrupt the protein-protein interactions to favor disassembly in the absence of a divalent metal. Successfully, a mutation was made that allowed the designed protein to be metal dependent for self-assembly. Nevertheless, an issue with this design is that it poorly incorporated ruthenium(II) into the tris Bpy complex forming [Ru(Bpy-ala)¬3]+2, which was one of the main goals of the original design. This thesis sets out to form TRI 05 I13S M6I which should uphold the same metal-dependence as its predecessor and should combine ruthenium (II) into the protein complex forming [Ru(Bpy-ala)¬3]+2. The thesis shows the success of formation and expression of TRI 05 I13S M6I in Escherichia coli cells. This thesis also reports several purification steps and procedures to not only purify TRI 05 I13S M6I but also removing both the His-tag sequence and Fe(II) from the protein. The thesis also shows that TRI 05 I13S M6I does not behave like its predecessor in that it is not metal dependent for self-assembly. While this may be true, this paper also reports the incorporation of ruthenium (II) in the protein structure. Though this may be the first time that ruthenium (II) has been recorded to be in the TRI 05 protein complex with a significant signal, it is still nowhere near the optimal fluorescence that small molecule Bpy can achieve by itself. The thesis reports potential conditions and a plan of attack that should drive this project forward into achieving an optimal signal of the [Ru(Bpy-ala)¬3]+2 complex in a TRI 05 protein scaffold.
ContributorsGrisingher, Dominic Waldo (Author) / Mills, Jeremy (Thesis director) / Nannenga, Brent (Committee member) / Lefler, Scott (Committee member) / School of Molecular Sciences (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2019-05