Theses and Dissertations
This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students.
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Description
With a quantum efficiency of nearly 100%, the electron transfer process that occurs within the reaction center protein of the photosynthetic bacteria Rhodobacter (Rh.) sphaeroides is a paragon for understanding the complexities, intricacies, and overall systemization of energy conversion and storage in natural systems. To better understand the way in which photons of light are captured, converted into chemically useful forms, and stored for biological use, an investigation into the reaction center protein, specifically into its cascade of cofactors, was undertaken. The purpose of this experimentation was to advance our knowledge and understanding of how differing protein environments and variant cofactors affect the spectroscopic aspects of and electron transfer kinetics within the reaction of Rh. sphaeroides. The native quinone, ubiquinone, was extracted from its pocket within the reaction center protein and replaced by non-native quinones having different reduction/oxidation potentials. It was determined that, of the two non-native quinones tested—1,2-naphthaquinone and 9,10- anthraquinone—the substitution of the anthraquinone (lower redox potential) resulted in an increased rate of recombination from the P+QA- charge-separated state, while the substitution of the napthaquinone (higher redox potential) resulted in a decreased rate of recombination.
ContributorsSussman, Hallie Rebecca (Author) / Woodbury, Neal (Thesis director) / Redding, Kevin (Committee member) / Lin, Su (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
In recent years, networked systems have become prevalent in communications, computing, sensing, and many other areas. In a network composed of spatially distributed agents, network-wide synchronization of information about the physical environment and the network configuration must be maintained using measurements collected locally by the agents. Registration is a process for connecting the coordinate frames of multiple sets of data. This poses numerous challenges, particularly due to availability of direct communication only between neighboring agents in the network. These are exacerbated by uncertainty in the measurements and also by imperfect communication links. This research explored statistically based registration in a sensor network. The approach developed exploits measurements of offsets formed as differences of state values between pairs of agents that share a link in the network graph. It takes into account that the true offsets around any closed cycle in the network graph must sum to zero.
ContributorsPhuong, Shih-Ling (Author) / Cochran, Douglas (Thesis director) / Berman, Spring (Committee member) / Barrett, The Honors College (Contributor) / Mechanical and Aerospace Engineering Program (Contributor)
Created2014-05
Description
Photosynthesis is a critical process that fixes the carbon utilized in cellular respiration. In higher plants, the immutans gene codes for a protein that is both involved in carotenoid biosynthesis and plastoquinol oxidation (Carol et al 1999, Josse et al 2003). This plastoquinol terminal oxidase (PTOX) is of great interest in understanding electron flow in the plastoquinol pool. In order to characterize this PTOX, polyclonal antibodies were developed. Expression of Synechococcus WH8102 PTOX in E. coli provided a useful means to harvest the protein required for antibody production. Once developed, the antibody was tested for limit of concentration, effectiveness in whole cell lysate, and overall specificity. The antibody raised against PTOX was able to detect as low as 10 pg of PTOX in SDS-PAGE, and could detect PTOX extracted from lysed Synechococcus WH8102. The production of this antibody could determine the localization of the PTOX in Synechococcus.
ContributorsKhan, Mohammad Iqbal (Author) / Moore, Thomas (Thesis director) / Redding, Kevin (Committee member) / Roberson, Robert (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
This thesis focused on understanding how humans visually perceive swarm behavior through the use of swarm simulations and gaze tracking. The goal of this project was to determine visual patterns subjects display while observing and supervising a swarm as well as determine what swarm characteristics affect these patterns. As an ultimate goal, it was hoped that this research will contribute to optimizing human-swarm interaction for the design of human supervisory controllers for swarms. To achieve the stated goals, two investigations were conducted. First, subjects gaze was tracked while observing a simulated swarm as it moved across the screen. This swarm changed in size, disturbance level in the position of the agents, speed, and path curvature. Second, subjects were asked to play a supervisory role as they watched a swarm move across the screen toward targets. The subjects determined whether a collision would occur and with which target while their responses as well as their gaze was tracked. In the case of an observatory role, a model of human gaze was created. This was embodied in a second order model similar to that of a spring-mass-damper system. This model was similar across subjects and stable. In the case of a supervisory role, inherent weaknesses in human perception were found, such as the inability to predict future position of curved paths. These findings are discussed in depth within the thesis. Overall, the results presented suggest that understanding human perception of swarms offers a new approach to the problem of swarm control. The ability to adapt controls to the strengths and weaknesses could lead to great strides in the reduction of operators in the control of one UAV, resulting in a move towards one man operation of a swarm.
ContributorsWhitton, Elena Michelle (Author) / Artemiadis, Panagiotis (Thesis director) / Berman, Spring (Committee member) / Barrett, The Honors College (Contributor) / Mechanical and Aerospace Engineering Program (Contributor)
Created2015-05
Description
The objective of this randomized, single-blind crossover study was to examine the effect of vinegar on the blood glucose response to meal ingestion. This study was associated with a companion study Is Apple Cider Vinegar Effective for Reducing Heartburn Symptoms Related to Gastroesophageal Reflux Disease. Glucose meters were utilized to measure blood glucose levels immediately prior to, and at four ½ hour intervals following meal ingestion. Previous studies have demonstrated that vinegar modulates the meal-time glucose response. Hence an alternative hypothesis was used: that a significant difference will be observed between the control and the vinegar groups. The results from the study were not significant likely due to a small sample size. The test meal eaten with a drink composed of vinegar diluted in water appeared to be most effective at decreasing the overall change in postprandial blood glucose. The vinegar drink also played a role in decreasing the peak glucose level at 30 minutes post-meal.
ContributorsPadgitt-Cobb, Lillian Katelyn (Author) / Johnston, Carol (Thesis director) / Redding, Kevin (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Coral reefs are diverse marine ecosystems, where reef building corals provide both the structure of the habitat as well as the primary production through their symbiotic algae, and alongside algae living on the reef itself, are the basis of the food web of the reef. In this way, coral reefs are the ocean's "forests" and are estimated to support 25% of all marine species. However, due to the large size of a coral reef, the relative inaccessibility and the reliance on in situ surveying methods, our current understanding of reefs is spatially limited. Understanding coral reefs from a more spatially complete perspective will offer insight into the ecological factors that contribute to coral reef vitality. This has become a priority in recent years due to the rapid decline of coral reefs caused by mass bleaching. Despite this urgency, being able to assess the entirety of a coral reef is physically difficult and this obstacle has not yet been overcome. However, similar difficulties have been addressed in terrestrial ecosystems by using remote sensing methods, which apply hyperspectral imaging to assess large areas of primary producers at high spatial resolutions. Adapting this method of remote spectral sensing to assess coral reefs has been suggested, but in order to quantify primary production via hyper spectral imaging, light-use efficiencies (LUEs) of coral reef communities need to be known. LUEs are estimations of the rate of carbon fixation compared to incident absorbed light. Here, I experimentally determine LUEs and report on several parameters related to LUE, namely net productivity, respiration, and light absorbance for the main primary producers in coral reefs surrounding Bermuda, which consist of algae and coral communities. The derived LUE values fall within typical ranges for LUEs of terrestrial ecosystems, with LUE values for coral averaging 0.022 ± 0.002 mol O2 mol photons-1 day-1 at a water flow rate of 17.5 ± 2 cm s^(-1) and 0.049 ± 0.011 mol O2 mol photons-1 day-1 at a flow rate of 32 ± 4 cm s^(-1) LUE values for algae averaged 0.0335 ± 0.0048 mol O2 mol photons-1 day-1 at a flow rate of 17.5 ± 2 cm s^(-1). These values allow insight into coral reef productivity and opens the door for future remote sensing applications.
ContributorsFlesher, David A (Author) / Neuer, Susanne (Thesis director) / Redding, Kevin (Committee member) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
With the revolution of low-cost microelectronics, rotary-wing vehicles have grown increasingly popular and important in the past two decades. With increased interest in quadcopters comes the need to for a systematic and rigorous framework to model, analyze, control, and design them. This thesis presents the beginning of such a framework.
The work presents the nonlinear equations of motion of a quadcopter. This includes the translational and rotational equations of motion, as well as an analysis of the nonlinear actuator dynamics. The work then analyzes the static properties of a quadcopter in forward flight equilibrium and shows how static properties change as physical properties of the vehicle are varied. Next, the dynamics of forward flight are linearized, and a dynamic analysis is provided.
After dynamic analysis, the work shows detailed hierarchical control system design trade studies, which includes attitude and translational inner-outer loop control. Among other designs, the following are presented: PD control, proportional control, pole-placement control. Each of these control architectures are employed for the inner loops and outer loops. The work also analyzes linear versus nonlinear simulation performance of a quadcopter, specifically for a step x-axis reference command. It is found that the nonlinear dynamics of the actuator cause significant discrepancy between linear and nonlinear simulation.
Finally, this thesis establishes directions for future graduate research. This includes hardware design, as well as moving toward design of a highly-maneuverable thrust-vectoring quadrotor which will be the focus of the proposed graduate PhD research. In summary, this thesis provides the beginning of a cohesive framework to model, analyze, control, and design quadcopters. It also lays the groundwork for graduate research and beyond.
The work presents the nonlinear equations of motion of a quadcopter. This includes the translational and rotational equations of motion, as well as an analysis of the nonlinear actuator dynamics. The work then analyzes the static properties of a quadcopter in forward flight equilibrium and shows how static properties change as physical properties of the vehicle are varied. Next, the dynamics of forward flight are linearized, and a dynamic analysis is provided.
After dynamic analysis, the work shows detailed hierarchical control system design trade studies, which includes attitude and translational inner-outer loop control. Among other designs, the following are presented: PD control, proportional control, pole-placement control. Each of these control architectures are employed for the inner loops and outer loops. The work also analyzes linear versus nonlinear simulation performance of a quadcopter, specifically for a step x-axis reference command. It is found that the nonlinear dynamics of the actuator cause significant discrepancy between linear and nonlinear simulation.
Finally, this thesis establishes directions for future graduate research. This includes hardware design, as well as moving toward design of a highly-maneuverable thrust-vectoring quadrotor which will be the focus of the proposed graduate PhD research. In summary, this thesis provides the beginning of a cohesive framework to model, analyze, control, and design quadcopters. It also lays the groundwork for graduate research and beyond.
ContributorsWallace, Brent (Author) / Rodriguez, Armando (Thesis director) / Berman, Spring (Committee member) / Electrical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12
Description
Diabesity is a global epidemic affecting millions worldwide. Diabesity is the term given to the link between obesity and Type II diabetes. It is estimated that ~90% of patients diagnosed with Type II diabetes are overweight or have struggled with excess body fat in the past. Type II diabetes is characterized by insulin resistance which is an impaired response of the body to insulin that leads to high blood glucose levels. Adipose tissue, previously thought of as an inert tissue, is now recognized as a major endocrine organ with an important role in the body's immune response and the development of chronic inflammation. It is speculated that adipose tissue inflammation is a major contributor to insulin resistance particular to Type II diabetes. This literature review explores the popular therapeutic targets and marketed drugs for the treatment of Type II diabetes and their role in decreasing adipose tissue inflammation. rAGE is currently in pre-clinical studies as a possible target to combat adipose tissue inflammation due to its relation to insulin resistance. Metformin and Pioglitazone are two drugs already being marketed that use unique chemical pathways to increase the production of insulin and/or decrease blood glucose levels. Sulfonylureas is one of the first FDA approved drugs used in the treatment of Type II diabetes, however, it has been discredited due to its life-threatening side effects. Bariatric surgery is a form of invasive surgery to rid the body of excess fat and has shown to normalize blood glucose levels. These treatments are all secondary to lifestyle changes, such as diet and exercise which can help halt the progression of Type II diabetes patients.
ContributorsRobles, Alondra Maria (Author) / Woodbury, Neal (Thesis director) / Redding, Kevin (Committee member) / Allen, James (Committee member) / Hendrickson, Kirstin (Committee member) / Sanford School of Social and Family Dynamics (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Antiviral lectins are potential candidates for future therapies against enveloped viruses like HIV due to their ability to recognize and bind glycans displayed on their surface. Cyanovirin-N (CVN), a lectin that specifically recognizes mannose-rich moieties, serves as a useful model for studying these glycan-recognition mechanisms. This study seeks to improve CVN's glycan-binding affinity by conjugating a boronic acid functional group to the N-terminus via N-terminal specific reductive alkylation by way of a benzaldehyde handle. However, large discrepancies were observed when attempting to confirm a successful conjugation, and further work is necessary to identify the causes and solutions for these issues.
ContributorsDiep, Tristan H (Author) / Ghirlanda, Giovanna (Thesis director) / Redding, Kevin (Committee member) / Mills, Jeremy (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
As Alzheimer’s disease (AD) increases in incidence, there is an increased investigation into the pathogenesis of the disease in hopes of finding a cure to the neurodegenerative disease. The two key hallmarks of AD consist of amyloid beta plaques and hyperphosphorylated tau fibrillary tangles. Amyloid beta is a peptide that is proteolytically cleaved from the type I transmembrane glycolytic amyloid precursor protein (APP). APP is highly conserved across species, suggesting the importance of APP in healthy brain functioning. However, when APP is cleaved through the amyloidogenic pathway it produces amyloid beta. The trafficking of APP within neurons has been a new endeavor for neurodegenerative disease research, as reduced retrograde trafficking of APP has been hypothesized to increase the likelihood of the amyloidogenic cleavage of APP, resulting in increased amyloid beta presence (Ye et al., 2017). The findings of this study suggest that transport of APP within neurons is significantly inhibited by increased extracellular glutamate concentration. The addition of human primary astrocytes within a human neuron co-culture allowed for significantly increased retrograde transport of APP within neurons, even within high glutamate conditions. These finding enhance the current field of research regarding astrocytes neuroprotective role within the brain, but bring attention to the role that astrocytes have upon regulation of the axonal transport of proteins within neurons.
ContributorsKlosterman, Katja Elisabeth (Author) / Ros, Alexandra (Thesis director) / Redding, Kevin (Committee member) / Watts College of Public Service & Community Solut (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12