Theses and Dissertations
This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students.
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- Creators: School of Life Sciences
Description
This creative project documents the changes to the Lopiano Habitat just north of Tempe Town Lake. Over the course of the project, the once restored wetland and desert habitat became overrun with debris and the plants and animals of the area were directly affected. Upon researching the city's choice to renovate the space, it was discovered that it was due to the increasing number of homeless and underserved individuals using the space for housing. Using photographs, the project displays the changed environment from lush habitat to trash-filled dirt patches. With the help of Julie Anand and Heather Green, I was able to select the best images to display as large scale prints, as well as small scale books that I constructed for my committee. Bioimagery was utilized to show what does not always meet the eye, and in some cases showed the effects that the demolition was having on the plant life, including what one of my committee members described as "an infection." They also acted as a metaphorical level to the photos in some cases, such as the hooks present on the regrown bamboo shoots that were slowly reclaiming the space. It was with the help of Dr. Page Baluch and her bioimagery lab that I was able to capture the smallest of details present on the samples I collected. The project serves as a potential starting point for other artists and community members to have a voice in the conversation and to hold the city accountable for their actions, especially when it comes to the underserved population. If they can spend so much time and funds into destroying what was once a beautiful habitat, why not put that effort into resources for the population they are trying to remove?
ContributorsGalbarski, Sydney (Author) / Anand, Julie (Thesis director) / Baluch, Debra (Committee member) / Green, Heather (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Phosphoinositol-Dependent Kinase 1 (PDK1) acts in conjunction with phosphorylated lipids such as Phosphoinositol-3,4,5-triphosphate (PIP3) to activate a variety of proteins that regulate mechanisms ranging from cell growth and survival to cytoskeletal rearrangement. In this investigation PDK1 was examined in the context of cellular division. The techniques of immunocytochemistry and live cell imaging were used to visualize the effects of the inhibition of PDK1 on division in HeLa cells. Division was impaired at metaphase of mitosis. The inhibited cells were unable to initiate anaphase cell-elongation ultimately leading to the flattening of spherical, metaphase cells. Preliminary studies with imunocytochemistry and live cell imaging suggested that insulin treatment reversed PDK1 inhibition, but the results were not statistically significant. Therefore, the recovery of PDK1 inhibition by insulin treatment could not be confirmed. Based on these observations a possible reason for the inability of the treated cells to complete cytokinesis could be the role of PDK1 in the Rho-kinase pathway that is required for the processes cell-elongation necessary for anaphase of mitosis.
ContributorsMasserano, Benjamin Max (Author) / Capco, David (Thesis director) / Baluch, Debra (Committee member) / Chandler, Douglas (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
In post-industrialized societies, increased consumption of fat-rich diets has been correlated to increasing rates of metabolic disorders, such as Type II Diabetes, which is further linked to insulin resistance. Due to this modern epidemic, it has become exceedingly important to learn more about these disorders with the ultimate goal of developing more effective treatments. With an overall focus on insulin resistance, the main purposes of this study were to (1) differentiate between two types of insulin resistance and their corresponding measurements and to (2) demonstrate metabolic changes that occur in response to overconsumption of a calorically dense diet. This was accomplished over a 23-week timespan by applying statistical analysis to periodically measured fasting insulin and blood glucose levels in rats fed either a high fat diet or low fat (chow) diet. Body weights were also recorded. The results of this study showed that rats fed a high fat diet experienced fasting hyperinsulinemia, hyperglycemia, and insulin resistance compared to rats fed a chow diet, and that the homeostatic model assessment (HOMA) scale and insulin-stimulated glucose disposal (ISGD) measure different types of insulin resistance. This study was unique in the fact that hepatic insulin resistance and peripheral insulin resistance were differentiated in the same rat.
ContributorsHenry, Lauren Elizabeth (Author) / Herman, Richard (Thesis director) / Baluch, Debra (Committee member) / School of Life Sciences (Contributor) / School of Music (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
This study was conducted to observe the effects of varying diets on weight regain after caloric restriction. Touted as a potentially effective non-invasive treatment to obesity, caloric restriction uses the gradual decrease in caloric intake to aid in weight loss. However, once a patient is taken off caloric restriction, a marked regain of weight regain occurs, nullifying the weight loss from caloric restriction. To find ways to suppress this weight regain, this study observed the effects of four different diets: low-fat diet (chow), high-fat diet (HFD), 0.5% concentration menthol infused chow, and 1% concentration menthol infused chow. Over a span of 3 years, 43 male Sprague-Dawley rats were placed through a strict feeding protocol: 3 weeks of chow food (3.1 kcal/gram), 8 or 12 weeks of HFD (5.42 kcal/gram), and caloric restriction for 4 weeks. Separate data analysis was conducted for the year 2017-2018, due to a slightly different protocol when compared to 2018-2019 and 2019-2020.
In 2017-2018, the results showed that 0.5% menthol (n=4) suppressed weight gain more effectively than both the baseline chow diet (n=4, p=0.022) and the HFD (n=4, p=0.027). Again in 2018-2020, the 0.5% menthol (n=6) showed promising results, showing significant suppression of weight gain when compared to chow (n=13, p=0.022). Unfortunately, the difference in weight gain in 1% menthol (n=6) was inconclusive when comparing to both chow and HFD. Although 1% menthol was inconclusive in its efficacy in suppressing weight regain, the promising results on 0.5% menthol show that menthol has the potential to be an effective treatment to both prevent rapid weight gain and maintain weight loss from caloric restriction.
In 2017-2018, the results showed that 0.5% menthol (n=4) suppressed weight gain more effectively than both the baseline chow diet (n=4, p=0.022) and the HFD (n=4, p=0.027). Again in 2018-2020, the 0.5% menthol (n=6) showed promising results, showing significant suppression of weight gain when compared to chow (n=13, p=0.022). Unfortunately, the difference in weight gain in 1% menthol (n=6) was inconclusive when comparing to both chow and HFD. Although 1% menthol was inconclusive in its efficacy in suppressing weight regain, the promising results on 0.5% menthol show that menthol has the potential to be an effective treatment to both prevent rapid weight gain and maintain weight loss from caloric restriction.
ContributorsLee, Justin (Author) / Van Horn, Wade (Thesis director) / Baluch, Debra (Committee member) / Herman, Richard (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05