This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students. 

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Lactate is a commonly known biochemical that is usually produced under anaerobic conditions. This makes it a useful marker for examining the possibility that Drosophila melanogaster undergoes natural hypoxic states during development due to the rate of growth. To analyze this observation and its potential for explaining developmental changes, a

Lactate is a commonly known biochemical that is usually produced under anaerobic conditions. This makes it a useful marker for examining the possibility that Drosophila melanogaster undergoes natural hypoxic states during development due to the rate of growth. To analyze this observation and its potential for explaining developmental changes, a lactate assay was used to quantify lactate produced across time points in the third larval instar and across early adulthood. Lactate assay results showed near-zero lactate levels for both larvae and adults. There were confounding factors present in larval lactate assays which made analysis difficult. However, the results of the adult lactate assays seem to indicate an inability to produce large amounts of lactate regardless of time point in adulthood, suggesting that adults do not naturally experience hypoxia during or after eclosion.

ContributorsWiertek, Marcellina Emilia (Author) / Harrison, Jon (Thesis director) / Angilletta, Michael (Committee member) / Talal, Stav (Committee member) / Historical, Philosophical & Religious Studies (Contributor) / Historical, Philosophical & Religious Studies, Sch (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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An organism's ability to maintain optimal body temperature is extremely important for sustaining physiological and behavioral processes necessary for survival. However, like other physiological systems, thermobiology can be influenced by the availability of resources. Water is a vital resource that has profound implications on many aspects of biological function, including

An organism's ability to maintain optimal body temperature is extremely important for sustaining physiological and behavioral processes necessary for survival. However, like other physiological systems, thermobiology can be influenced by the availability of resources. Water is a vital resource that has profound implications on many aspects of biological function, including thermoregulatory pathways. However, water availability has a tendency fluctuate within any given ecosystem. While several studies have investigated the influence of water availability on a range of thermoregulatory pathways, very little attention has been given to its influence on Voluntary Maximum Temperature (VMT). We investigated the effects of dehydration on Voluntary Maximum Temperature in a captive population of Gila monsters (Heloderma suspectum). Gila monsters are large-bodied, desert dwelling lizards that experience periods of seasonal dehydration. Additionally, the effects of dehydration on their physiology and behavior have been extensively studied. We hypothesized that dehydration would reduce VMT. As expected, there was a significant decrease in exit temperature as blood osmolality increased. This is presumed to be in an effort to decrease water loss. Adaptations that allow desert dwelling organisms to conserve water are highly advantageous due to seasonal water constraints. Our findings offer insight on how the behavior of these organisms may change in response to changes in climate.
ContributorsHartson, Callie Elizabeth (Author) / DeNardo, Dale (Thesis director) / Angilletta, Michael (Committee member) / Camacho, Agus (Committee member) / School of Life Sciences (Contributor) / School of Human Evolution and Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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In 2007, the Center for Biological Diversity (CBD) petitioned the United States Fish and Wildlife Service (USFWS) and the California Department of Fish and Wildlife (CDFW) to list the American pika (Ochotona princeps) as an endangered species. After several petition denials, the petition was evaluated during both 90-day, and 12-month

In 2007, the Center for Biological Diversity (CBD) petitioned the United States Fish and Wildlife Service (USFWS) and the California Department of Fish and Wildlife (CDFW) to list the American pika (Ochotona princeps) as an endangered species. After several petition denials, the petition was evaluated during both 90-day, and 12-month reviews. Ultimately, both petitions were denied and the pika was not given protection under the Endangered Species Act (ESA). During the petitioning years, 2007 through 2013, there were many newspaper publications, press releases, and blog entries supporting the listing of the pika. Information published by these media ranged from misleading, to scientifically inaccurate. The public was swayed by these publications, and showed their support for listing the pika during the public comment period throughout the 12-month status review in California. While the majority of the public comments were in favor of listing the pika, there were a few letters that criticized the CBD for making a poster child out of a "cute" species. During the 12-month status review, the CDFW contacted pika experts and evaluated scientific literature to gain an understanding of the American pika's status. Seven years after the original petition, the CDFW denied listing the pika on the grounds that the species is not expected to become extinct in the next few decades. This case serves as an example where a prominent organization, the CBD, petitions to list a species that does not warrant protection. Their goal of making the pika the face of climate change failed when species was examined.
ContributorsBasso, Samantha Joy (Author) / Smith, Andrew (Thesis director) / Minteer, Ben (Committee member) / Angilletta, Michael (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2014-05
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Description
Water-balance is a critical but understudied consideration for animals reproducing in dry environments, as females invest a significant amount of water into their offspring. What makes water especially challenging, is that few animals are known to have true water storage, whereas energy as fat storage is well-documented. Recent studies have

Water-balance is a critical but understudied consideration for animals reproducing in dry environments, as females invest a significant amount of water into their offspring. What makes water especially challenging, is that few animals are known to have true water storage, whereas energy as fat storage is well-documented. Recent studies have suggested the possibility that, when drinking water is scarce, animals can catabolize their muscles, thereby extracting cellular water. In this study, the aim was to show this as a potential method used by animals reproducing in dry environments to cope with dehydration and still produce a clutch. Children's pythons (Antaresia childreni) were used to investigate this phenomenon due to the fact that they experience two, distinctive, reproductive phases- vitellogenesis (when protein and energy are mobilized and invested into the yolk) and gravidity (when the major water investment into the egg occurs, as well as egg shelling). Other factors that make them excellent candidates are that they are pure capital breeders (don't eat during the reproductive season) and can withstand periods of water deprivation that far outlast their reproductive gravid phase. Reproductive and non-reproductive females were deprived of water for the duration of gravidity, and their mass decrease, epaxial muscle shrinkage, blood osmolality, total protein, uric acid, triglycerides and ketones were measured at the onset of each reproductive stage; these values were compared to their water-provided counterparts. Water-deprived females experienced greater mass loss, epaxial muscle loss, blood plasma osmolality, and uric acid than water-provided females. These findings suggest that muscle catabolism is used as a method of dealing with water-deprivation during gravidity.
ContributorsKaminsky, Brittany Michele (Author) / DeNardo, Dale (Thesis director) / Angilletta, Michael (Committee member) / Brusch, George (Committee member) / School of Life Sciences (Contributor) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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Description
The migratory grasshopper (Melanoplus sanguinipes) is one of the most economically important grasshoppers in the western rangelands of the United States (US), capable of causing incredible amounts of damage to crops and rangelands. While M. sanguinipes has been the focus of many research studies, areas like field nutritional physiology and

The migratory grasshopper (Melanoplus sanguinipes) is one of the most economically important grasshoppers in the western rangelands of the United States (US), capable of causing incredible amounts of damage to crops and rangelands. While M. sanguinipes has been the focus of many research studies, areas like field nutritional physiology and ecology, and interactions between nutritional physiology and biopesticide resistance have very little research. This dissertation presents a multifaceted approach through three research-driven chapters that examine the nutritional physiology of M. sanguinipes and how it interacts with an entomopathogenic fungus for grasshopper management, as well as the challenges of using biopesticides for grasshopper management. Using the Geometric Framework for Nutrition (GFN), I established baseline macronutrient intake for M. sanguinipes, both in laboratory and field populations. Through this work, I found that field and lab populations can exhibit different protein (p) to carbohydrate (c) ratios, or Intake Targets (ITs), but that the field populations had ITs that matched the nutrients available in their environment. I also used the GFN to show that infections with the fungal entomopathogen Metarhizium robertsii DWR2009 did not alter ITs in M. sanguinipes. Although, when confined to carbohydrate- or protein-biased diets, infected grasshoppers had a slightly extended lifespan relative to grasshoppers fed balanced protein:carbohydrate diets. Interestingly, in a postmortem for the grasshopper, the fungus was only able to effectively sporulate on grasshoppers fed the 1p:1c diets, suggesting that grasshopper diet can have substantial impacts on the spread of fungal biopesticides throughout a population, in the absence of any inhibitory abiotic factors. Lastly, I examined the major barriers to fungal and microsporidian biopesticide usage in the United States, including low efficacy, thermal and environmental sensitivity, non-target effects, unregistered or restricted use, and economic or accessibility barriers. I also explored potential solutions to these challenges. This dissertation's focus on Melanoplus sanguinipes and Metarhizium roberstii Strain DWR2009, generates new information about how nutritional physiology and immunology intersect to impact M. sanguinipes performance. The methodology in each of the experimental chapters provides a framework for examining other problematic grasshopper species, by determining baseline nutritional physiology, and coupling nutrition with immunology to maximize the effectiveness of biological pesticides.
ContributorsZembrzuski, Deanna (Author) / Cease, Arianne (Thesis advisor) / Harrison, Jon (Committee member) / Angilletta, Michael (Committee member) / Jaronski, Stefan (Committee member) / Arizona State University (Publisher)
Created2023
Description

Due to what is known as the “biologically desert fallacy” and the pervasive westernized ideal of wilderness that has influenced widespread American Conservation culture for millennia, urban areas have long been deemed as areas devoid of biodiversity. However, cities can contribute significantly to regional biodiversity and provide vital niches for

Due to what is known as the “biologically desert fallacy” and the pervasive westernized ideal of wilderness that has influenced widespread American Conservation culture for millennia, urban areas have long been deemed as areas devoid of biodiversity. However, cities can contribute significantly to regional biodiversity and provide vital niches for wildlife, illuminating the growing awareness that cities are crucial to the future of conservation and combating the global biodiversity crisis. In terms of the biodiversity crisis, bats are a relevant species of concern. In many studies, different bat species have been broadly classified according to their ability to adapt to urban environments. There is evidence that urban areas can filter bat species based on traits and behavior, with many bats possessing traits that do not allow them to live in cities. The three broad categories are urban avoiders, urban adapters, or urban exploiters based upon where their abundance is highest along a gradient of urban intensity. A common example of an urban exploiter bat is a Mexican Free-tailed bat, which can thrive and rely on urban environments and it is found in the Phoenix Metropolitan area. Bats are important as even in urban environments they play vital ecological roles such as cactus pollination, insect management, and seed dispersal. Bat Crazy is a thesis project focused on urban enhancement and the field of urban biodiversity. The goals of this thesis are to observe how bio-conscious urban cities that work to promote species conservation can serve as a positive tool to promote biodiversity and foster community education and engagement for their urban environment.

ContributorsKaiser, Nicole (Author) / Senko, Jesse (Thesis director) / Angilletta, Michael (Committee member) / Lynch, John (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / School of Life Sciences (Contributor)
Created2023-05