Theses and dissertations

This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students. 

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Genome sequencing and analysis of the psychrophilic anoxygenic phototrophic bacterium Rhodoferax antarcticus sp. ANT.BR

Description
Rhodoferax antarcticus strain ANT.BR, a purple nonsulfur bacterium isolated from a microbial mat in Ross Island, Antarctica, is the first described anoxygenic phototrophic bacterium that is adapted to cold habitats and is the first beta-proteobacterium to undergo complete genome sequencing. R. antarcticus has unique absorption spectra and there are no…

Rhodoferax antarcticus strain ANT.BR, a purple nonsulfur bacterium isolated from a microbial mat in Ross Island, Antarctica, is the first described anoxygenic phototrophic bacterium that is adapted to cold habitats and is the first beta-proteobacterium to undergo complete genome sequencing. R. antarcticus has unique absorption spectra and there are no obvious intracytoplasmic membranes in cells grown phototrophically, even under low light intensity. Analysis of the finished genome sequence reveals a single chromosome (3,809,266 bp) and a large plasmid (198,615 bp) that together harbor 4,262 putative genes. The genome contains two types of Rubiscos, Form IAq and Form II, which are known to exhibit quite different kinetic properties in other bacteria. The presence of multiple Rubisco forms could give R. antarcticus high metabolic flexibility in diverse environments. Annotation of the complete genome sequence along with previous experimental results predict the presence of structural genes for three types of light-harvesting (LH) complexes, LH I (B875), LH II (B800/850), and LH III (B800/820). There is evidence that expression of genes for the LH II complex might be inhibited when R. antarcticus is under low temperature and/or low light intensity. These interesting condition-dependent light-harvesting apparatuses and the control of their expression are very valuable for the further understanding of photosynthesis in cold environments. Finally, R. antarcticus exhibits a highly motile lifestyle. The genome content and organization of all putative polar flagella genes are characterized and discussed.
ContributorsZhao, Tingting, M.S (Author) / Touchman, Jeffrey (Thesis advisor) / Rosenberg, Michael (Committee member) / Redding, Kevin (Committee member) / Stout, Valerie (Committee member) / Arizona State University (Publisher)
Created2011
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Recombinant Expression of the Kinase MARS1 in Saccharomyces Cerevisiae

Description
In algae, the Mutant Affecting Retrograde Signaling (MARS1) Kinase plays a critical role in the chloroplast unfolded protein response (cpUPR) when the chloroplast faces proteotoxic stress4. The MARS1 protein is relatively unknown in terms of structure and function. However, there has been ample research performed on the main pathway associated…

In algae, the Mutant Affecting Retrograde Signaling (MARS1) Kinase plays a critical role in the chloroplast unfolded protein response (cpUPR) when the chloroplast faces proteotoxic stress4. The MARS1 protein is relatively unknown in terms of structure and function. However, there has been ample research performed on the main pathway associated with the MARS1 protein, the cpUPR. The exact mechanism of why MARS1 is necessary for the cpUPR is still unknown. Our structural and biochemical studies will help develop a better understanding of the MARS1 structure, and the role it plays in the cpUPR. The MARS1 expression construct will be assembled following the yeast golden gate (yGG) assembly protocol. Here, we will attempt to recombinantly express MARS1 kinase in Saccharomyces cerevisiae to provide insights into the protein.
ContributorsHeeres, Nicholas (Author) / Mazor, Yuval (Thesis director) / Chiu, Po Lin (Committee member) / Redding, Kevin (Committee member) / Barrett, The Honors College (Contributor) / School of Molecular Sciences (Contributor)
Created2022-05