Theses and Dissertations
This collection includes both ASU Theses and Dissertations, submitted by graduate students, and the Barrett, Honors College theses submitted by undergraduate students.
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- All Subjects: Diagnostics
Description
The healthcare system in this country is currently unacceptable. New technologies may contribute to reducing cost and improving outcomes. Early diagnosis and treatment represents the least risky option for addressing this issue. Such a technology needs to be inexpensive, highly sensitive, highly specific, and amenable to adoption in a clinic. This thesis explores an immunodiagnostic technology based on highly scalable, non-natural sequence peptide microarrays designed to profile the humoral immune response and address the healthcare problem. The primary aim of this thesis is to explore the ability of these arrays to map continuous (linear) epitopes. I discovered that using a technique termed subsequence analysis where epitopes could be decisively mapped to an eliciting protein with high success rate. This led to the discovery of novel linear epitopes from Plasmodium falciparum (Malaria) and Treponema palladium (Syphilis), as well as validation of previously discovered epitopes in Dengue and monoclonal antibodies. Next, I developed and tested a classification scheme based on Support Vector Machines for development of a Dengue Fever diagnostic, achieving higher sensitivity and specificity than current FDA approved techniques. The software underlying this method is available for download under the BSD license. Following this, I developed a kinetic model for immunosignatures and tested it against existing data driven by previously unexplained phenomena. This model provides a framework and informs ways to optimize the platform for maximum stability and efficiency. I also explored the role of sequence composition in explaining an immunosignature binding profile, determining a strong role for charged residues that seems to have some predictive ability for disease. Finally, I developed a database, software and indexing strategy based on Apache Lucene for searching motif patterns (regular expressions) in large biological databases. These projects as a whole have advanced knowledge of how to approach high throughput immunodiagnostics and provide an example of how technology can be fused with biology in order to affect scientific and health outcomes.
ContributorsRicher, Joshua Amos (Author) / Johnston, Stephen A. (Thesis advisor) / Woodbury, Neal (Committee member) / Stafford, Phillip (Committee member) / Papandreou-Suppappola, Antonia (Committee member) / Arizona State University (Publisher)
Created2014
Description
In-situ fatigue damage diagnosis and prognosis is a challenging problem for both metallic and composite materials and structures. There are various uncertainties arising from material properties, component geometries, measurement noise, feature extraction techniques, and modeling errors. It is essential to manage and incorporate these uncertainties in order to achieve accurate damage detection and remaining useful life (RUL) prediction.
The aim of this study is to develop an integrated fatigue damage diagnosis and prognosis framework for both metallic and composite materials. First, Lamb waves are used as the in-situ damage detection technique to interrogate the damaged structures. Both experimental and numerical analysis for the Lamb wave propagation within aluminum are conducted. The RUL of lap joints under variable and constant fatigue loading is predicted using the Bayesian updating by incorporating damage detection information and various sources of uncertainties. Following this, the effect of matrix cracking and delamination in composite laminates on the Lamb wave propagation is investigated and a generalized probabilistic delamination size and location detection framework using Bayesian imaging method (BIM) is proposed and validated using the composite fatigue testing data. The RUL of the open-hole specimen is predicted using the overall stiffness degradation under fatigue loading. Next, the adjoint method-based damage detection framework is proposed considering the physics of heat conduction or elastic wave propagation. Different from the classical wave propagation-based method, the received signal under pristine condition is not necessary for estimating the damage information. This method can be successfully used for arbitrary damage location and shape profiling for any materials with higher accuracy and resolution. Finally, some conclusions and future work are generated based on the current investigation.
The aim of this study is to develop an integrated fatigue damage diagnosis and prognosis framework for both metallic and composite materials. First, Lamb waves are used as the in-situ damage detection technique to interrogate the damaged structures. Both experimental and numerical analysis for the Lamb wave propagation within aluminum are conducted. The RUL of lap joints under variable and constant fatigue loading is predicted using the Bayesian updating by incorporating damage detection information and various sources of uncertainties. Following this, the effect of matrix cracking and delamination in composite laminates on the Lamb wave propagation is investigated and a generalized probabilistic delamination size and location detection framework using Bayesian imaging method (BIM) is proposed and validated using the composite fatigue testing data. The RUL of the open-hole specimen is predicted using the overall stiffness degradation under fatigue loading. Next, the adjoint method-based damage detection framework is proposed considering the physics of heat conduction or elastic wave propagation. Different from the classical wave propagation-based method, the received signal under pristine condition is not necessary for estimating the damage information. This method can be successfully used for arbitrary damage location and shape profiling for any materials with higher accuracy and resolution. Finally, some conclusions and future work are generated based on the current investigation.
ContributorsPeng, Tishun (Author) / Liu, Yongming (Thesis advisor) / Chattopadhyay, Aditi (Committee member) / Mignolet, Marc (Committee member) / Papandreou-Suppappola, Antonia (Committee member) / Tang, Pingbo (Committee member) / Arizona State University (Publisher)
Created2016