Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
Displaying 1 - 10 of 39
Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The relationship between parent and child is one that has been studied intensively for years. Much of the previous research in this field has quantified the parent-child relationship through self-report measures, with a subsample coding behavior from videotape and averaging individual scores across the entire parent-child interaction. Using a dynamic systems approach, we attempted to gain a deeper understanding of the parent-child relationship by quantifying the relationship in terms of dyadic patterns using the software Gridware. We then used these dyadic patterns to predict internalizing and externalizing behaviors in eight-year-old twin children. Dyadic relationship patterns predicted externalizing behaviors such as aggression and conduct disorder (i.e., frequency and stability within negative attractor states, and infrequency and low stability in positive attractor states), but not internalizing behaviors. Findings provide a method for capturing variance in parent-child interactions that is important for children's externalizing behaviors. Future studies should utilize these patterns in understanding risk and resilience family processes for children's mental health and well being.
ContributorsEccles, Jenna Christine (Author) / Lemery-Chalfant, Kathryn (Thesis director) / Knight, George (Committee member) / Spinrad, Tracy (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor)
Created2015-05
Description
Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
We examined the relations between maternal depression, discipline practices, and toddler mental health outcomes, specifically competence and total problem behavior. Ethnicity was considered as a moderator in all analyses. For the first time, ethnicity was considered as a moderator of the heritability of toddler competence and total problem behavior. The data came from the Arizona Twin Project. A subsample containing only Caucasian (66%) and Hispanic (34%; 87% of Mexican descent) participants was used. Primary caregivers (>95% mothers) reported on levels of maternal depression, discipline practices, and their twins' competency and problem behaviors. It was hypothesized that maternal depression would be associated with less competency and more problem behaviors in toddlers; inductive discipline practices would be associated with higher competency and fewer problem behaviors; and punitive discipline practices would be associated with lower competency and more problem behaviors. Ethnicity was predicted to moderate only the relation between discipline practices and toddler mental health. Consistent with predictions, maternal depression predicted less competency and more problem behaviors, and inductive discipline predicted higher competency and fewer problem behaviors, while punitive discipline predicted lower competency and more problem behaviors. Ethnicity moderated the relation between maternal depression at 12 months and total problem behaviors. The heritability of competence and total problem behavior varied across the Caucasian and Hispanic samples.
ContributorsChon, Sarah Maria (Author) / Lemery-Chalfant, Kathryn (Thesis director) / Knight, George (Committee member) / Bradley, Robert (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / School of International Letters and Cultures (Contributor)
Created2015-05
Description
Background: Measles virus (MV) infections are the main cause of vaccine-preventable death in children younger than 5 years. The World Health Organization (WHO) has estimated there are over 20 million cases of measles every year. Currently, diagnostic methods rely on enzyme immunoassays (EIA) to detect IgM or IgG Abs in serum. These commercial assays measure reactivity against the immunodominant N antigen and can have a false negative rates of 20-30%. Centralized testing by clinical labs can delay rapid screening in an outbreak setting. This study aims to develop a rapid molecular diagnostic assay to detect IgG reactive to five individual MV proteins representing 85% of the measles proteome. Methods: MV genes were subcloned into pANT_cGST vector to generate C-terminal GST fusion proteins. Single MV cistrons were expressed using in vitro transcription/translation (IVTT) with human cell lysate. Expression of GST-tagged proteins was measured using a sandwich ELISA for GST expression using relative light units (RLUs) as readouts. Single MV antigens were used as bait to determine the IgG-dependent reactivity in 12 serum samples obtained from immunized animals with previously determined neutralization titer (NT) and the correlation between NT and ELISA reactivity was determined. Results: Protein expression of five measles genes of interest, M, N, F, H, and L, was measured. L exhibited the strongest protein expression with an average RLU value of 4.34 x 10^9. All proteins were expressed at least 50% greater than control (2.33 x 10^7 RLU). As expected, reactivity against the N was the highest, followed by reactivity against M, F, H and L. The best correlation with NT titer was reactivity against F (R^2 = 0.62). Conclusion: These data indicate that the expression of single MV genes M, N, F, H, and L are suitable antigens for serologic capture analysis of measles immunity.
ContributorsMushtaq, Zuena (Author) / Anderson, Karen (Thesis director) / Reyes del Valle, Jorge (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
The relations between prenatal risk factors and postnatal risk and protective factors and infant outcomes were examined. Mothers, primarily of low-income background and Latina ethnicity, were interviewed within 24 hours of giving birth, and then again when their infants were five and nine months of age. The relations between prenatal stress, postnatal environment, and infant maturity and temperament were analyzedusing a multiple regression model. We controlled for the covariates: mother's education level and infant's birth weight. Maternal prenatal risk factors predicted lower infant Regulation and lower Developmental Maturity at nine months. Maternal postnatal risks did not predict infant outcomes, but maternal expectations for their child provided a significant association for three of the four infant outcomes: Regulation, Surgency, and Infant Developmental Maturity. The results underscore the importance of prenatal stress holding its significance with the addition of postnatal measures. Future studies would need to explore deeper into a multitude of postnatal factors, in order to accurately portray associations between maternal prenatal stress and infant health.
ContributorsTran, Melissa (Author) / Lemery-Chalfant, Kathryn (Thesis director) / Luecken, Linda (Committee member) / McDonald, Kristy (Committee member) / Barrett, The Honors College (Contributor)
Created2012-05
Description
Background: Human papillomavirus (HPV) is the cause of 99.7% of cervical cancers. Research of cervical cancer has made this disease mostly curable in the developing world. Head and neck cancer, which is increasingly caused by HPV, still is associated with a mortality rate of 50,000 in the US annually. This study proposed to evaluate the biology of HPV-16 in head and neck tumors by using RT-qPCR to measure the RNA expression and its relation to physical status of the virus. Methods: This study was to develop an assay that uses RT-qPCR to determine the quantitative expression of HPV-16 RNA coding for proteins E1, E2, E4, E5, E6, and E7 in tumor samples. The assay development started with creation of primers. It went on to test the primers on template DNA through traditional PCR and then on DNA from HPV-16 positive cell lines, SiHa and CaSki, using RT-qPCR. This paper also describes the troubleshooting methods taken for the PCR reaction. Once the primers are verified, the RT-qPCR process can be carried out on RNA purified from tumor samples. Results: No primer sets have been confirmed to produce a product through PCR or RT-qPCR. The primer sequences match up correctly with known sequences for HPV-16 E1, E2, E4, E5, E6, and E7. RT-qPCR showed results consistent with the hypothesis. Conclusion: The RT-qPCR protocol must be optimized to confirm the primer sequences work as desired. Then primers will be used to study physical status and RNA expression in HPV-positive and HPV-negative head and neck tumor samples. This assay can help shed light on which proteins are expressed most in tumors of the head and neck and will aid in the development of future screening and treatment options.
ContributorsKhazanovich, Jakob (Author) / Anderson, Karen (Thesis director) / Mangone, Marco (Committee member) / Sundaresan, Sri Krishna (Committee member) / Barrett, The Honors College (Contributor)
Created2015-05
Description
Cancer poses a significant burden on the global health system and represents a leading cause of death worldwide. For late-stage cancers, the traditional treatments of chemotherapy, radiation, and surgery are not always viable, and they can pose unnecessary health risks to the patients. New immunotherapies, such as adoptive cell transfer, are being developed and refined to treat such cancers. T cell immunotherapies in particular, where a patient’s T cell lymphocytes are isolated and amplified to be re-infused into the patient or where human cell lines are engineered to express T cell receptors for the recognition of common cancer antigens, are being expanded on because for some cancers, they could be the only option. Constructing an optimal pipeline for cloning and expression of antigen-specific TCRs has significant bearing on the efficacy of engineered cell lines for ACT. Adoptive T cell transfer, while making great strides, has to overcome a diverse T cell repertoire – cloning and expressing antigen-specific TCRs can mediate this understanding. Having identified the high frequency FluM1-specific TCR sequences in stimulated donor PBMCs, it was hypothesized that the antigen-specific TCR could be reconstructed via Gateway cloning methods and tested for expression and functionality. Establishing this pipeline would confirm an ability to properly pair and express the heterodimeric chains. In the context of downstream applications, neoantigens would be used to stimulate T cells, the α and β chains would be paired via single-cell or bulk methods, and instead of Gateway cloning, the CDR3 hypervariable regions α and β chains alone would be co-expressed using Golden Gate assembly methods.
ContributorsHirneise, Gabrielle Rachel (Author) / Anderson, Karen (Thesis director) / Mason, Hugh (Committee member) / Hariadi, Hugh (Committee member) / School of Life Sciences (Contributor, Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
While previous research has investigated the influence parenting styles have on child behavior, there has not been consistent findings on how child behavior in return influences parenting. This study goes beyond the literature by examining bidirectional influences of combined dyad for emotional availability and early problem behaviors (composited across 12 and 30 months) predicting parental warmth, authoritarian parenting, internalizing, externalizing and ADHD symptoms at age eight. This study also examined whether genetic or environmental factors were driving these behaviors. Participants were from the ongoing Arizona Twin Project (N=340 twin children). 25% of the twins were monozygotic, 35% were same-sex dizygotic, and 35% were opposite-sex dizygotic twins. Preliminary correlations showed bidirectional effects between early emotional availability, problem behaviors and parental warmth, authoritarian parenting, internalizing, externalizing and ADHD symptoms at age eight; however, once twin dependence and covariates were controlled for, the bidirectional effects were no longer significant. One important finding emerged: early problem behaviors were predictive of later problem behaviors at eight years. The study also found that externalizing and ADHD symptoms were more heritable than emotional availability, early problem behaviors, and internalizing symptoms. Therefore, interventions should be developed addressing the environmental influences that contribute to early problem behaviors.
ContributorsKaur, Navneet (Author) / Lemery-Chalfant, Kathryn (Thesis director) / Miadich, Samantha (Committee member) / Davis, Mary (Committee member) / Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Approximately 95% of Americans have at least one sibling (Weaver, Coleman, & Ganong, 2003), making it more likely that children grow up with a sibling than with a father (Lyon, 2009). It is therefore somewhat surprising to learn that sibling relationships have not been a central focus of psychological research, especially considering the fact that parent-child and peer relationships have been studied so extensively. There is no doubt that parents and peers have profound effects on children's emotional, psychological and social wellbeing, but siblings have important effects as well. By middle childhood, children spend more time with their siblings than they do with their parents (Pike, Coldwell, & Dunn, 2005). The sibling relationship is one of the longest and most lasting relationships that we as humans have. Approximately 78% of Americans over the age of sixty still have contact with at least one sibling (Cicirelli, 1995). Unlike parents, siblings are often in our lives until the end of our lifespan and, unlike friends, we do not choose them. They act as teachers, friends, and critics, just to name a few, and they are often a sounding board off of which we can test our ideas and behaviors. The focus of the current study is on the twin sibling relationship quality in middle childhood and in adolescence and its implications for individual adjustment, specifically in the realm of prosocial and aggressive behaviors. I evaluated twin sibling cooperation and conflict at both age 7-8 years and age 12-14 years and then examined prosocial and aggressive tendencies concurrently and longitudinally to study the strength of the association between the two. This study also aimed to better understand the extent to which prosocial behavior and aggression are influenced by genetic and environmental factors.
ContributorsDitto, Kayla Rhiannon Loretta Cam (Author) / Lemery-Chalfant, Kathryn (Thesis director) / Presson, Clark (Committee member) / Valiente, Carlos (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor)
Created2013-05