Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
Displaying 1 - 10 of 47
Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
This interdisciplinary thesis examines the possible relationship between the public speaking experience for women and the gender gap in political ambition. First, a historical analysis of women public speakers ranging from the 1800s to the Suffragettes to female politicians in the 1900s reveals a pattern of female public speakers in politics receiving extreme criticism for their communicative behavior. The thesis then turns to the socialization of young girls, highlighting how gameplay in children translates into gendered communicative behavior in adult women. Next, an examination of the pedagogy of public speaking showcases how the public speaking experience is different for women than it is for men, and how public speaking traditionally is taught in a masculine style. Then, through a review of the literature on the gender gap in political ambition, it is seen that not only are women severely underrepresented in political office in the United States, but women have far less political ambition than men. And a case study of the 2008 presidential primaries and elections, highlighting modern women in politics, demonstrates that the few women who are politically ambitious in the 21st century face criticism that mirrors those faced by political women decades and centuries prior. Finally, the thesis offers possible solutions to changing the experience of women as public speakers and fostering political ambition in women.
ContributorsPatton, Ashley Crystal (Author) / Gruber, Diane (Thesis director) / Wentzel, Bonnie (Committee member) / Barrett, The Honors College (Contributor) / School of Social and Behavioral Sciences (Contributor)
Created2015-05
Description
"The Hunger Games: What a Dystopic World Reveals about Modern Society" is an interdisciplinary thesis that examines the impossibility of revolutionary stories or concepts in popular culture by specifically analyzing the Hunger Games project. First, an analysis of what young adult fiction is and how it is written is provided. The formulaic way in which modern adolescent literature is written provides the basic structure for Suzanne Collins' Hunger Games trilogy. The second chapter examines the main character of the Hunger Games series, Katniss Everdeen. The way in which this young female heroine relinquishes her independence and courage due to being consistently undermined by the men and political leaders in her life is traced by following the development of the story throughout the three novels. The third chapter of the thesis delves into how the entire Hunger Games project of novels and films fits into current popular culture. An analysis of the mass production of the novels, and then turning the books into films, merchandise, and further commercialization of the story is discussed in detail throughout the chapter. Finally, the thesis discusses the responsibility authors of young adult literature should assume when addressing a young impressionable audience and how Collins took advantage of the position she had in telling the story of Katniss Everdeen.
ContributorsHeath, Elisabeth Rose (Author) / Gruber, Diane (Thesis director) / Bixby, Patrick (Committee member) / Roy, Sohinee (Committee member) / Barrett, The Honors College (Contributor) / School of Social and Behavioral Sciences (Contributor) / School of Humanities, Arts, and Cultural Studies (Contributor)
Created2015-05
Description
Background: Measles virus (MV) infections are the main cause of vaccine-preventable death in children younger than 5 years. The World Health Organization (WHO) has estimated there are over 20 million cases of measles every year. Currently, diagnostic methods rely on enzyme immunoassays (EIA) to detect IgM or IgG Abs in serum. These commercial assays measure reactivity against the immunodominant N antigen and can have a false negative rates of 20-30%. Centralized testing by clinical labs can delay rapid screening in an outbreak setting. This study aims to develop a rapid molecular diagnostic assay to detect IgG reactive to five individual MV proteins representing 85% of the measles proteome. Methods: MV genes were subcloned into pANT_cGST vector to generate C-terminal GST fusion proteins. Single MV cistrons were expressed using in vitro transcription/translation (IVTT) with human cell lysate. Expression of GST-tagged proteins was measured using a sandwich ELISA for GST expression using relative light units (RLUs) as readouts. Single MV antigens were used as bait to determine the IgG-dependent reactivity in 12 serum samples obtained from immunized animals with previously determined neutralization titer (NT) and the correlation between NT and ELISA reactivity was determined. Results: Protein expression of five measles genes of interest, M, N, F, H, and L, was measured. L exhibited the strongest protein expression with an average RLU value of 4.34 x 10^9. All proteins were expressed at least 50% greater than control (2.33 x 10^7 RLU). As expected, reactivity against the N was the highest, followed by reactivity against M, F, H and L. The best correlation with NT titer was reactivity against F (R^2 = 0.62). Conclusion: These data indicate that the expression of single MV genes M, N, F, H, and L are suitable antigens for serologic capture analysis of measles immunity.
ContributorsMushtaq, Zuena (Author) / Anderson, Karen (Thesis director) / Reyes del Valle, Jorge (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Background: Human papillomavirus (HPV) is the cause of 99.7% of cervical cancers. Research of cervical cancer has made this disease mostly curable in the developing world. Head and neck cancer, which is increasingly caused by HPV, still is associated with a mortality rate of 50,000 in the US annually. This study proposed to evaluate the biology of HPV-16 in head and neck tumors by using RT-qPCR to measure the RNA expression and its relation to physical status of the virus. Methods: This study was to develop an assay that uses RT-qPCR to determine the quantitative expression of HPV-16 RNA coding for proteins E1, E2, E4, E5, E6, and E7 in tumor samples. The assay development started with creation of primers. It went on to test the primers on template DNA through traditional PCR and then on DNA from HPV-16 positive cell lines, SiHa and CaSki, using RT-qPCR. This paper also describes the troubleshooting methods taken for the PCR reaction. Once the primers are verified, the RT-qPCR process can be carried out on RNA purified from tumor samples. Results: No primer sets have been confirmed to produce a product through PCR or RT-qPCR. The primer sequences match up correctly with known sequences for HPV-16 E1, E2, E4, E5, E6, and E7. RT-qPCR showed results consistent with the hypothesis. Conclusion: The RT-qPCR protocol must be optimized to confirm the primer sequences work as desired. Then primers will be used to study physical status and RNA expression in HPV-positive and HPV-negative head and neck tumor samples. This assay can help shed light on which proteins are expressed most in tumors of the head and neck and will aid in the development of future screening and treatment options.
ContributorsKhazanovich, Jakob (Author) / Anderson, Karen (Thesis director) / Mangone, Marco (Committee member) / Sundaresan, Sri Krishna (Committee member) / Barrett, The Honors College (Contributor)
Created2015-05
Description
The lack of diversity for minorities and especially women of color is astounding in the film industry. Film is supposed to imitate life, but that can't happen successfully unless more stories are told about different kinds of people. The following thesis focuses on the diversity struggle, specifically for black women by analyzing Hollywood films and critiquing the structure of the film industry. I chose to focus on films made in Hollywood because they are extremely ubiquitous, meaning they can reach many different people from around the world. Hollywood films set the tone and build impressions in other countries about what certain types of people look and act like and the stereotypes associated with these films reach millions of minorities as well. Essentially, solving the diversity problem will make a larger impact not only in America, but around the world. The four chapters of this thesis focus on the history of blacks in film, representation of black women, black women behind the screen, and improvement for the future of the industry.
ContributorsCook, Sydni Nicole (Author) / Gruber, Diane (Thesis director) / St. Clair, Charles (Committee member) / Gray, Kishonna (Committee member) / School of Humanities, Arts, and Cultural Studies (Contributor) / School of Social and Behavioral Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Cancer poses a significant burden on the global health system and represents a leading cause of death worldwide. For late-stage cancers, the traditional treatments of chemotherapy, radiation, and surgery are not always viable, and they can pose unnecessary health risks to the patients. New immunotherapies, such as adoptive cell transfer, are being developed and refined to treat such cancers. T cell immunotherapies in particular, where a patient’s T cell lymphocytes are isolated and amplified to be re-infused into the patient or where human cell lines are engineered to express T cell receptors for the recognition of common cancer antigens, are being expanded on because for some cancers, they could be the only option. Constructing an optimal pipeline for cloning and expression of antigen-specific TCRs has significant bearing on the efficacy of engineered cell lines for ACT. Adoptive T cell transfer, while making great strides, has to overcome a diverse T cell repertoire – cloning and expressing antigen-specific TCRs can mediate this understanding. Having identified the high frequency FluM1-specific TCR sequences in stimulated donor PBMCs, it was hypothesized that the antigen-specific TCR could be reconstructed via Gateway cloning methods and tested for expression and functionality. Establishing this pipeline would confirm an ability to properly pair and express the heterodimeric chains. In the context of downstream applications, neoantigens would be used to stimulate T cells, the α and β chains would be paired via single-cell or bulk methods, and instead of Gateway cloning, the CDR3 hypervariable regions α and β chains alone would be co-expressed using Golden Gate assembly methods.
ContributorsHirneise, Gabrielle Rachel (Author) / Anderson, Karen (Thesis director) / Mason, Hugh (Committee member) / Hariadi, Hugh (Committee member) / School of Life Sciences (Contributor, Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
This thesis seeks to compile the best practices described in popular advice literature for women in leadership. The analyzed books are roughly categorized into two types, which will be labeled the Take No Prisoners and Girl Power approaches. The thesis then further transitions into discussions on three separate issues that women face during their careers. The first issue addresses the initial mindset that women must overcome in order to begin a path to success: "Striving for Perfection." Continuing from that is the issue of "Working with Others," which refers to the need to network and engage in mentoring. It represents the many stepping stones that all business leaders must use, but that womyn need to navigate differently than men. Next, the thesis will discuss the topic of "Assertiveness." While this advice is helpful, it becomes apparent that not all of it is applicable to women in the Millennial generation. To address this, the thesis will conclude with an analysis of the texts' relevancy to Millennial women, as they begin their careers and/or begin to enter leadership roles within their careers. This section will also include a prediction on how such professional advice texts will differ once Millennial women progress in their careers and pass down their own knowledge and experiences.
ContributorsShannon, Alyssa Marie (Author) / Gruber, Diane (Thesis director) / Gaffney, Cynthia (Committee member) / W.P. Carey School of Business (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
A home holds so much more meaning and power than the physical structure of a house. As much as our personal space serves as an extension of ourselves, it also affects us. Furthermore, whichever state a home environment is in has a major impact on psychological well-being. This thesis is an investigation of the idea of home design as a means of addressing psychological anxiety through the point of view of a college student. The information is divided into three chapters; which are Overview of Relevant Scholarly Literature, Design Philosophies, and Personal Experience. Within the scholarly literature, well-being and anxiety are two trends in the studies of environmental psychology, positive psychology, and the humanities. There is still limited knowledge in these areas, so it is important to expand the understanding of the home environment's influence. Based on this research, well-known philosophies, and personal experience, design philosophies are an effective way to potentially improve well-being and reduce anxiety, especially for college students. While Hygge and Wabi-sabi are both design philosophies rising in recognition, Feng Shui is already widespread around the world. Some of the recommendations discoverer were to add cozy décor and lower lighting options to soften the room, get rid of extra clutter taking up space, or bring in nature with greenery and fresh flowers. However, these objects have countless interpretations and there is not a single correct answer. In the end, adjusting the space to be individualized will bring more comfort and these efforts will begin to make a difference in the user’s state of mind.
ContributorsDemaagd, Brittni Nicole (Author) / Gruber, Diane (Thesis director) / Ramsey, Ramsey Eric (Committee member) / School of Social and Behavioral Sciences (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05