Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
Displaying 1 - 10 of 38
Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Background: Measles virus (MV) infections are the main cause of vaccine-preventable death in children younger than 5 years. The World Health Organization (WHO) has estimated there are over 20 million cases of measles every year. Currently, diagnostic methods rely on enzyme immunoassays (EIA) to detect IgM or IgG Abs in serum. These commercial assays measure reactivity against the immunodominant N antigen and can have a false negative rates of 20-30%. Centralized testing by clinical labs can delay rapid screening in an outbreak setting. This study aims to develop a rapid molecular diagnostic assay to detect IgG reactive to five individual MV proteins representing 85% of the measles proteome. Methods: MV genes were subcloned into pANT_cGST vector to generate C-terminal GST fusion proteins. Single MV cistrons were expressed using in vitro transcription/translation (IVTT) with human cell lysate. Expression of GST-tagged proteins was measured using a sandwich ELISA for GST expression using relative light units (RLUs) as readouts. Single MV antigens were used as bait to determine the IgG-dependent reactivity in 12 serum samples obtained from immunized animals with previously determined neutralization titer (NT) and the correlation between NT and ELISA reactivity was determined. Results: Protein expression of five measles genes of interest, M, N, F, H, and L, was measured. L exhibited the strongest protein expression with an average RLU value of 4.34 x 10^9. All proteins were expressed at least 50% greater than control (2.33 x 10^7 RLU). As expected, reactivity against the N was the highest, followed by reactivity against M, F, H and L. The best correlation with NT titer was reactivity against F (R^2 = 0.62). Conclusion: These data indicate that the expression of single MV genes M, N, F, H, and L are suitable antigens for serologic capture analysis of measles immunity.
ContributorsMushtaq, Zuena (Author) / Anderson, Karen (Thesis director) / Reyes del Valle, Jorge (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Background: Human papillomavirus (HPV) is the cause of 99.7% of cervical cancers. Research of cervical cancer has made this disease mostly curable in the developing world. Head and neck cancer, which is increasingly caused by HPV, still is associated with a mortality rate of 50,000 in the US annually. This study proposed to evaluate the biology of HPV-16 in head and neck tumors by using RT-qPCR to measure the RNA expression and its relation to physical status of the virus. Methods: This study was to develop an assay that uses RT-qPCR to determine the quantitative expression of HPV-16 RNA coding for proteins E1, E2, E4, E5, E6, and E7 in tumor samples. The assay development started with creation of primers. It went on to test the primers on template DNA through traditional PCR and then on DNA from HPV-16 positive cell lines, SiHa and CaSki, using RT-qPCR. This paper also describes the troubleshooting methods taken for the PCR reaction. Once the primers are verified, the RT-qPCR process can be carried out on RNA purified from tumor samples. Results: No primer sets have been confirmed to produce a product through PCR or RT-qPCR. The primer sequences match up correctly with known sequences for HPV-16 E1, E2, E4, E5, E6, and E7. RT-qPCR showed results consistent with the hypothesis. Conclusion: The RT-qPCR protocol must be optimized to confirm the primer sequences work as desired. Then primers will be used to study physical status and RNA expression in HPV-positive and HPV-negative head and neck tumor samples. This assay can help shed light on which proteins are expressed most in tumors of the head and neck and will aid in the development of future screening and treatment options.
ContributorsKhazanovich, Jakob (Author) / Anderson, Karen (Thesis director) / Mangone, Marco (Committee member) / Sundaresan, Sri Krishna (Committee member) / Barrett, The Honors College (Contributor)
Created2015-05
Description
Cancer poses a significant burden on the global health system and represents a leading cause of death worldwide. For late-stage cancers, the traditional treatments of chemotherapy, radiation, and surgery are not always viable, and they can pose unnecessary health risks to the patients. New immunotherapies, such as adoptive cell transfer, are being developed and refined to treat such cancers. T cell immunotherapies in particular, where a patient’s T cell lymphocytes are isolated and amplified to be re-infused into the patient or where human cell lines are engineered to express T cell receptors for the recognition of common cancer antigens, are being expanded on because for some cancers, they could be the only option. Constructing an optimal pipeline for cloning and expression of antigen-specific TCRs has significant bearing on the efficacy of engineered cell lines for ACT. Adoptive T cell transfer, while making great strides, has to overcome a diverse T cell repertoire – cloning and expressing antigen-specific TCRs can mediate this understanding. Having identified the high frequency FluM1-specific TCR sequences in stimulated donor PBMCs, it was hypothesized that the antigen-specific TCR could be reconstructed via Gateway cloning methods and tested for expression and functionality. Establishing this pipeline would confirm an ability to properly pair and express the heterodimeric chains. In the context of downstream applications, neoantigens would be used to stimulate T cells, the α and β chains would be paired via single-cell or bulk methods, and instead of Gateway cloning, the CDR3 hypervariable regions α and β chains alone would be co-expressed using Golden Gate assembly methods.
ContributorsHirneise, Gabrielle Rachel (Author) / Anderson, Karen (Thesis director) / Mason, Hugh (Committee member) / Hariadi, Hugh (Committee member) / School of Life Sciences (Contributor, Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Modern American environmental social movements have strived for a better world for nearly fifty years, pushing a philosophy of careful resource use and limited consumption as an alternative to the pollution and degradation that has so far accompanied global industrialization. The reach of these movements is broad and the topic they cover is one that aligns with the values and beliefs of many; it is thus quite confusing that they've been so unsuccessful. This thesis was a response to that apparent contradiction, exploring why movements have not been as successful as both they and the public initially desired. It began by defining what social movements are and how they emerge or find success, then provided a brief history of environmentalism in America, and the different successes and failures that occurred before and after the first Earth day in 1970. Finally, it explored some of the reasons environmentalism was unsuccessful, and found that while structural barriers like politics and business interests played a role in movement outcomes, the tactics of different groups were at least partially to blame. Once this was concluded, the author used the perspectives of different activists to propose ways to enhance the quality of current movements and allow them to continue to make progress well into the future. In order to expand the audience of this thesis, the author is also working on a children;s book that illustrates many of the important themes that he hopes to convey to the public. Though drafted, the book is incomplete as of the date that documents are due for Barrett review.
ContributorsGuy, Joel D (Author) / Cloutier, Scott (Thesis director) / Josh, MacFayden (Committee member) / Department of English (Contributor) / School of Sustainability (Contributor) / School of Geographical Sciences and Urban Planning (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
Current farming demographics in the United States indicate an aging and overwhelmingly white group of farmers, stimulating the need for engaging a younger and more diverse population. There is an opportunity to engage these populations through farm-based internship and apprenticeship programs, which are immersive programs on small-scale, sustainable farms. These programs are unique in providing hands-on training, housing, meals, and a stipend in return for labor, presenting a pathway to social empowerment. The potential outcomes of increasing diversity and inclusion in farm programs are absent from the research on the benefits of diversity and inclusion in other work environments, such as the corporate setting. This paper presents the results of a study aimed at determining levels of diversity and inclusion in United States farm-based internship programs, and the viability of these programs as an effective opportunity to engage marginalized young people in farming. The study of 13 farm owners and managers across the U.S. found that the participants are focused on fostering education and training, environmental benefits, and a sense of community in their respective programs. All participants either want to establish, or believe they currently have, an inclusive workplace on their farm, but also indicated a barrier to inclusivity in the lack of a diverse applicant pool. Future recommendations for removing that barrier and involving more young, diverse interns include increased outreach and access to these programs, the use of inclusive language, and further research.
ContributorsLascola, Dania (Co-author) / Biel, Braden (Co-author) / Cloutier, Scott (Thesis director) / MacFadyen, Joshua (Committee member) / School of International Letters and Cultures (Contributor) / School of Geographical Sciences and Urban Planning (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Food waste is a growing global issue that exemplifies an unsustainable system of resource loss in landfills which eventually breaks down into the greenhouse gas of methane. Approaching landfill diversion of food waste on the local level requires innovative solutions based on public and private partnerships. This thesis project explored how the City of Tempe's Grease Cooperative could provide a model of restaurant partnership and third-party service to tackle not just restaurant grease waste in water, but food waste in the solid waste stream. This used other city-run food waste collection systems as examples, and it relied on the input and support of multiple municipal stakeholders in its design. Using an existing food waste collection service in the Phoenix metropolitan area, the research was collected during a month-long observational pilot study of four Tempe restaurants, where data ranged from trash bin differences to kitchen staff sizes. The results of the pilot were compiled for the benefit of the collection service, the City of Tempe, and the involved restaurants to demonstrate potential obstacles to a currently small, but scalable, collection service, and potential solutions that will make the service more efficient and attractive to new customers. Future research goals include expanding the pilot's reach and information through stronger partnerships and collaborative data collection in Tempe, providing a guide to a food waste collection cooperative within Tempe, and promoting large scale diversion of food waste from restaurants both through prevention and nutrient recycling. The final paper was submitted for publication to the Solutions journal, as an example of "On the Ground" implementation of solutions.
ContributorsAndersen, Annika Emmaline (Author) / Cloutier, Scott (Thesis director) / Eakin, Hallie (Committee member) / School of Historical, Philosophical and Religious Studies (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Research has long supported the idea that parks contribute to physical and mental well-being. Evidence has shown that the presence of parks reduces stress, positively impacts health, and can lower the risk of crime in neighborhoods. There have been studies that discuss variables that impact the accessibility and quality of parks in neighborhoods such as ethnicity, income, and gender. More recently, research has delved into the impact of parks on individual's happiness. Findings imply that the desire for happiness may be satisfied by park visitation and provide evidence that suggests the diversity of park activities is a significant contributor to happiness. This study aims to identify how varying park designs contribute to individual happiness. Three hypotheses are presented: 1) frequency of park visits is positively correlated with life satisfaction, 2) park structure impacts efficacy of parks to promote happiness, and 3) adults travel to parks specifically to improve their mood. Hypothesis 1 is used to understand the relationship between park visitation and overall satisfaction, hypothesis 2 aims to identify how the physical structure of the park contributes to personal happiness, and hypothesis 3 provides an understanding for what motivates adults to visit parks. This study's results indicate that there is no significant correlation between frequency of park visits and life satisfaction, nor is there a significant correlation between physical park structure and increased happiness. While an insignificant amount of participants reported traveling to parks specifically to enhance their mood, the majority of participants indicated traveling to the park to participate in an activity that positively affects their happiness. This study can act as a tool for urban planners to get an idea of why people visit parks and which features they use while they're there. This information can provide guidance when deciding what to include in future parks, utilizing their budget in a way that maximizes community use and happiness.
ContributorsTapia, Isabella Andrea (Author) / Cloutier, Scott (Thesis director) / LePine, Marcie (Committee member) / Department of Management and Entrepreneurship (Contributor) / School of Public Affairs (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
Public participation is lauded as a keystone of sustainability policy and community development. Sustainability issues span all sectors of society and are best addressed at the local level, which makes community involvement and participation necessary for building local sustainability strategies. But do public participation events actually foster meaningful connections among those who attend? How can we as sustainability experts empower communities to share their knowledge about the place where they live? This project starts by considering at gaps in public participation processes that prevent members of a community from building a sense of trust. Major gaps identified in the public participation process include a lack of attention to underlying power dynamics, unaddressed social tensions, and a lack of focus on the co-creation of knowledge. These gaps lead to a lack of trust between facilitators and participants, and prevents participants from feeling invested in the process and forming meaningful connections with their fellow participants. Based on the gaps identified in public participation processes, the second part of this project focused on hosting a workshop that would bring people together in an effort to rebuild trust. The workshop centered around the meaning of community and sense of place, as these topics are relevant to the health and relationships of communities. The event was hosted on Arizona State University's Tempe campus, and the participants were all connected to the university in some way (student, faculty, or alumni). A pre-workshop survey was sent out to participants to gauge favorite places on campus and what made those places meaningful. The workshop itself was broken into two parts: Part One focused on the building a trusting space for the workshop and unpacking the definition of community in a group discussion. Part Two included two mapping exercises that engaged participants in how the land around ASU's Tempe campus had changed over time, followed by a discussion about how the history of land affects communities. A post-workshop survey was sent out two weeks after the event to see how participants had incorporated lessons from the workshop, if at all. The workshop process brought up several interesting areas for further research. One outcome of the discussion in Part One of the workshop was that the participants tended to think of community in terms of relationships rather than place. People also interacted differently based on how confident they were in their knowledge of the topic at hand, whether expert or informal. Public participation workshops like this have implications for how governments, businesses and schools approach stakeholder engagement. With the right balance of power and co-creation of knowledge, public participation events can become places for members of a community to rebuild trust in each other and the institutions that govern them.
ContributorsBaker, Hailey Louise (Author) / Cloutier, Scott (Thesis director) / Morrison, Beth Ann (Committee member) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05