Barrett, The Honors College Thesis/Creative Project Collection

On March 11th, COVID-19 was declared a pandemic by the World Health Organization. The ensuing months saw an extensive allocation of resources toward combating the virus and the development of a vaccine. Despite extensive research on SARS-CoV-2, there remains little information regarding the implications of SARS-CoV-2 gastrointestinal shedding on COVID-19 disease. It is hypothesized that SARS-CoV-2 RNA is shed in the stool for up to several weeks and that viral protein persists in the GI tract. This study also explored calprotectin and zonulin levels, markers of inflammation, and intestinal permeability, respectively, to assess if increased viral shedding is associated with elevated levels of either. This study utilized RT-qPCR assays to confirm the presence of viral RNA. Subsequently, RT-qPCR positive samples were heat-inactivated and SARS-CoV-2 spike detection enzyme-linked immunosorbent assay (ELISA) was used to ascertain viral protein shedding. Additional ELISA was performed to assess zonulin and calprotectin levels. Results indicated that 30 of the 758 unique samples were confirmed SARS-CoV-2 positive by RT-qPCR. Spike protein was ultimately not detected by ELISA. Additionally, no significant increase in zonulin was observed in patient samples when comparing RT-qPCR positive and negative Samples. A notable upwards trend approaching significance in calprotectin levels existed for patients who tested positive for SARS-CoV-2 by RT-qPCR, though, it was found that no correlation existed between SARS-CoV-2 copy number and calprotectin levels. Understanding the interaction between SARS-CoV-2 and the GI tract may therefore have significant clinical implications and this study demonstrates the need for additional studies to garner a more comprehensive understanding.

Drylands cover almost half of the land surface on Earth, yet there is still little understood of the processes in these ecosystems. This project studied the impact of macroclimate (precipitation and temperature in large regions) in comparison to microclimate (the climate under canopy versus in the open) to learn more about the drivers of litter decomposition in drylands.

Due to the COVID-19 pandemic, pre-health individuals around the world encountered a range of challenges. Research and internship opportunities were cancelled, clinical experience was unreachable, and prerequisites became more demanding in a remote setting. I myself was working in a research lab in Switzerland when the pandemic was declared, resulting in my career-altering internship to be cut short six months. My life-long friend, Alejandra, had the same experience and reached out to me with an extraordinary idea to unite and empower pre-health individuals on a national level. With my skills in event planning combined with her vision, we built the National Pre-Health Conference (NPHC): a 3-day virtual event for pre-health individuals to explore medical careers and learn how to pursue their professional goals, particularly during these uncertain times. We held our inaugural conference with the theme A Future in Medicine in 2020 with over 1000 attendees from around the country. In 2021, we held our second-annual conference with the theme Unity in Healthcare with over 1000 attendees as well. In addition to planning the second-annual NPHC, I employed pre-event and post-event surveys to assess the confidence level of attendees before and after the conference in healthcare experience, research experience, standardized testing, community service, academics, essay writing, and completing graduate/professionals school applications. We found that NPHC improved the confidence level of attendees in all categories. Overall, understanding how NPHC benefits pre-health students will help our team improve for future conferences.

This thesis/creative project is a guide for other universities to follow in making their campuses more inclusive and accessible via maps. This guide will be offered in different formats (ex – PDF, a website, audio, etc.) to accommodate the disabled community. Hopefully, this guide will serve as inspiration and starting point for universities around the country to better the college experience for all.

The COVID-19 pandemic has generated alarming increases in psychological distress and alcohol use behaviors and has caused the greatest increases in depression and anxiety symptoms among college students. Prior studies have examined the impact of COVID-19 broadly on mental health and alcohol use outcomes; however, few studies have examined these impacts in college students. Previous studies have examined individual factors that could moderate the relation between COVID-19 related stressors and mental health and alcohol use outcomes, but knowledge is lacking regarding the role of emotion regulation. The present study aimed to examine the role of emotion regulation in the relation between both COVID-19 stressful experiences and COVID-19 related worry and mental health and alcohol use outcomes, and to explore racial/ethnic differences in their associations. Four hierarchical multiple regression models were conducted to assess main effects of COVID-19 stressors and emotion regulation, as well as moderation of the effect of emotion regulation on depression symptoms, anxiety symptoms, alcohol consumption, and alcohol use disorder (AUD) symptoms during the past year. COVID-19 related worry was associated with greater symptoms of both mental health outcomes, whereas COVID-19 related stressful experiences were associated with both mental health outcomes, more alcohol consumption, and more AUD symptoms. Difficulties in emotion regulation had significant main effects on mental health outcomes and AUD symptoms, but not alcohol consumption. Hispanic/Latinx students reported higher experiences of both COVID-19 related stressors, but consumed less alcohol than did White/European students. This study provides further insight into the nature of COVID-19 related stressors and their subsequent impacts. Implications for prevention and intervention on college campuses are discussed.

U.S. border colonias, otherwise known as Disadvantaged Unincorporated Communities, are rural settlements along the U.S. Mexico border with substandard housing conditions. Colonia residents often face inadequate access to necessities such as appropriate shelter, septic and sewer systems, and potable water. Water insecurity in colonias poses a particularly difficult challenge for residents who require clean water not only for consumption, but also household use in sanitation and hygienic practices. As of 2015, an estimated 30% of over five million US colonia residents lack access to clean drinking water, resulting in health complications and unsanitary living conditions. Preliminary health data collected indicates that due to water insecurity, colonia residents are more likely to contract gastrointestinal disease, be exposed to carcinogenic compounds from contaminated water, and experience psychosocial distress. Yet more comprehensive research needs to be conducted to understand the full breadth of the public health issue. A scoping review on water insecurity in colonias has not been completed before and could be beneficial in informing policymakers and other stakeholders on the severity of the situation while advising possible solutions.