Barrett, The Honors College Thesis/Creative Project Collection

Measuring changes in concentration within a dynamic system can be accomplished with a simple Arduino powered system. Currently, the system is utilized in cyanobacteria CO2 fixation experiments, where the fixation rates of multiple cultures can be measured simultaneously. The system employs solenoids in parallel and can be applied for n number of outlet streams, all are connected to one large manifold which feeds to a CO2 concentration probe. In the future, the system can be modified to fit other simple dynamic gas systems.

Triple Negative Breast Cancer (TNBC), indicated by the absence of estrogen, progesterone and human epidermal growth factor receptor 2 (HER2), is the most aggressive form of breast cancer characterized by high rates of metastasis and low survival. Among those diagnosed with TNBC, 34% contain Inhibitor of Growth 4 (ING4) deletion that is associated with poor patient outcomes. We previously showed that ING4 negatively regulates NF-B in breast cancer. Previous studies show parthenolide, a compound found in feverfew (Tanacetum parthenium) to inhibit NF-B in cervical and gastric cancer. We hypothesized that parthenolide inhibits cytokine-induced activation of NF-B in ING4 deficient TNBC cells. To test the hypothesis, previously established vectors, v2, ING4 wildtype and v2h1, ING4-deleted were synthesized in MDA-MB 231, a TNBC cell line, using a CRISPR/Cas9 system. Inflammatory cytokines, IL-1 and TNF, were tested in ING4 wildtype or ING4 deleted cells for elicited phosphorylation of NF-B, proliferation, and migration in the presence or absence of parthenolide. The results showed that TNF or IL-1 induced translocation phosphorylation of NF-B regardless of ING4 deletion. ING4 inhibited proinflammatory cytokine induced pp65, consistent with previous studies demonstrating the negative regulation of NF-B in ING4-sufficent cell lines. We found the optimal working dose of parthenolide, 100nM, had no effect on cell proliferation in the presence or absence of IL-1. Parthenolide inhibited IL-1induced phosphorylation of NF-B regardless of ING4 deletion. Parthenolide inhibited TNF-induced phosphorylation of NF-B in ING4-deleted cell lines. Moreover, parthenolide induced migration of TNBC cells regardless of ING4 presence of absence. TNF and parthenolide treated samples in ING4-deleted cell lines were found to inhibit cell migration to basal level. These results demonstrate the difference in inhibitory mechanism of parthenolide in induced phosphorylation of NF-B through proinflammatory cytokines TNF or IL-1This is demonstrated by the exclusivity of parthenolide inhibition of TNF induced phosphorylation of NF-B in ING4-deleted TNBC cell line. In contrast, parthenolide inhibition of IL-1 induced phosphorylation of NF-B occurred regardless of ING4 deletion. These results may inhibit parthenolide as an alternative to those with ING4-deleted TNBC due to its role in inducing cancer phenotype cell migration.

This thesis examines statements made about immigration and mental health in Americanah by Chimamanda Ngozi Adichie and The Book of Unknown Americans by Cristina Henriquez. Americanah by Chimamanda Ngozi Adichie follows a young Nigerian immigrant as she navigates her move to the U.S. and explores the meaning of belonging and identity in the U.S. and Nigeria. Henriquez’s The Book of Unknown Americans is about a Mexican family that immigrates to Delaware in order to secure better treatment for their daughter and touches on the nuances of the Latinx immigrant identity in the U.S. Both of these texts feature the lack of resources and support available for immigrants of color which eventually lead the characters to return to their country of origin. This thesis posits that Adichie and Henriquez are both suggesting in their respective works that the U.S. fails to ensure the success and well-being of immigrants which leads to a deterioration of mental health and feelings of not belonging. A Portrait of Neglect considers the real life implications of Adichie’s and Henriquez’s ideas and the impact of their representations of immigration and mental health.

For those families that rely on food banks as their main source of groceries for the week, it is not uncommon for the parents or children to not know how to prepare the ingredients in a way that can provide as much nutritional value as possible. A cookbook with a collection of recipes that specialize in using ingredients commonly found in food banks would be one way to help improve the physical and mental health of families while also teaching children how to cook and prepare nutritious meals at home. I was inspired to create these recipes because I wanted to work with food banks and pantries to help educate their clients about healthy eating and cooking techniques. I wanted to show families that they can cook various meals using many of the same ingredients in different ways. I also wanted to develop recipes that encourage children to cook, become more familiar with different food items, and improve their relationship with food since a significant portion of clients are children, meaning they grow up relying on the ingredients food banks offer. After finding out which recipes and other nutritional aid programs currently exist, I spoke with a few different food banks to learn what types of food are typically distributed from food banks. From there, I drafted a list of recipes, worked with AZ Health Zone to analyze the nutrients for each recipe, and revised the recipes to better meet the nutrition standards of AZ Health Zone. As of now, a handful of food pantries, including the AZ Health Zone, agreed to share my total of 9 recipes (in English and Spanish) with their clients.

It is often a passion for caring for others that drives people towards the profession of nursing in the first place. At some point, however, this desire to care for other people takes over, and nurses soon forget how to properly care for themselves. It was asked: If students are better prepared with and more encouraged to use healthy coping mechanisms and integrative modalities to deal with their daily lives, are they more likely to continue to care for themselves properly as time goes on? Ample research was conducted illuminating the prevalence of this worldwide problem and highlighting potential solutions noted by credible sources. An initiative was formed with the goal of improving the self care practices of nursing students at Arizona State University (ASU) with the greater intent of promoting a healthier work environment as these students advance into their future careers. The Arizona Student Chapter of the American Holistic Nurses Association (AHNA) at ASU was formed, and it serves as the vessel for this self-care health-care mission.

Every day, the earth’s oceans are being destroyed. Pollution, fishing, sonar, and many other man-made factors have caused detrimental effects to the most crucial of the ocean’s ecosystems. While more individuals are becoming aware of these problems, additional support is needed to help protect the ocean’s many unique creatures. The purpose of this honors thesis exhibition is to continue to shine light on human negligence towards threatened ocean creatures. The three artworks in this thesis show the descent of diversity and life of these marine creatures over time. By showcasing the different ways in which whales, rays, and corals have been affected by human impact, this thesis and subsequent art pieces will help to continue to enhance one’s understanding of the importance of marine conservation.