Barrett

Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.

Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.

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HPV Antibodies as Novel Biomarkers for the Detection of Cervical Neoplasia

Description
Background: The human papillomavirus (HPV) is the cause of virtually all cervical cancer, with over 520,000 new cases and 275,000 deaths annually. Although there are at least 200 unique HPV strains, only “high-risk” types, may progress to cancer. Serum antibodies to HPV oncoproteins are stable and specific markers that may…

Background: The human papillomavirus (HPV) is the cause of virtually all cervical cancer, with over 520,000 new cases and 275,000 deaths annually. Although there are at least 200 unique HPV strains, only “high-risk” types, may progress to cancer. Serum antibodies to HPV oncoproteins are stable and specific markers that may be able to detect high-grade cervical intraepithelial neoplasia (CIN3). Biomarkers have potential as a rapid, point-of-care HPV screening tool for low resource areas in the way that traditional cytology cannot, and HPV DNA testing is not yet able to.
Methods: We have designed a multiplexed magnetics programmable bead ELISA (MagProBE) to profile the immune responses of the proteins from 11 high-risk HPV types and 2 low-risk types—106 genes in total. HPV genes were optimized for human expression and either built with PCR or commercially purchased, and cloned into the Gateway-compatible pANT7_cGST vector for in vitro transcription/translation (IVTT) in a MagProBE array. Anti-GST antibody (Ab) labeling was then used to measure gene expression.
Results: 53/106 (50%) HPV genes have been cloned and tested for expression of protein. 91% of HPV proteins expressed at levels above the background control (MFI = 2288), and the mean expression was MFI = 4318. Codon-optimized genes have also shown a 20% higher expression over non-codon optimized genes.
Conclusion: Although this research is ongoing, it suggests that gene optimization may improve IVTT expression of HPV proteins in human HeLa lysate. Once the remaining HPV proteins have been expression confirmed, the cDNA for each gene will be printed onto slides and tested in serologic assays to identify potential Ab biomarkers to CIN3.
ContributorsResnik, Jack Isiah (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Purushothaman, Immanuel (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2013-05
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Strong Mothers Strong Teeth: Improving the Lack of Oral Health Education Among Mothers

Description
Strong Mothers Strong Teeth is an initiative to educate mothers and pregnant women about the importance of maintaining their own and their child(ren)’s oral health. This project presents evidence that shows a lack of oral health education among mothers about how to care for their oral health while pregnant and…

Strong Mothers Strong Teeth is an initiative to educate mothers and pregnant women about the importance of maintaining their own and their child(ren)’s oral health. This project presents evidence that shows a lack of oral health education among mothers about how to care for their oral health while pregnant and their child’s oral health post birth. The recognition and identification of these disparities led to the content deemed necessary to be included in the education of mothers and pregnant women. By collecting and analyzing pamphlets and information gathered from health clinics and homeless shelters in Arizona and California, we created our own pamphlets based on analysis of the effectiveness of the information using content analysis and Flesch-Kincaid readability scores. This led to the creation of two pamphlets to educate mothers on oral health care, the first focused on preventing tooth decay in women during their pregnancy and for their baby, post-birth, and the second provided a timeline guide on oral health for the mother and child.
ContributorsBlair, Grace (Co-author) / Patel, Toral (Co-author) / Loebenberg, Abby (Thesis director) / Magee, Mitch (Committee member) / School of Social Transformation (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12