Barrett

Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.

Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.

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Characterizing electrical parameters from two human-derived glioblastoma cell lines in vitro using electrochemical impedance spectroscopy

Description
High-grade gliomas are highly aggressive central nervous system (CNS) malignancies with high fatality rates if left untreated. There is currently a lack of reliable diagnostic tools to characterize the diffuse cell populations commonly found in these tumors. Here, we report that electrochemical impedance spectroscopy (EIS) can be used in an…

High-grade gliomas are highly aggressive central nervous system (CNS) malignancies with high fatality rates if left untreated. There is currently a lack of reliable diagnostic tools to characterize the diffuse cell populations commonly found in these tumors. Here, we report that electrochemical impedance spectroscopy (EIS) can be used in an in vitro system to analyze changes in the impedance contributed by the extracellular matrix (ECM) of two glioblastoma cell lines: GBM 22 and GBM 115. EIS was more effective at resolving differences in impedance from GBM 115 cells than GBM 22 cells, which depended on both cell confluency and frequency. However, differences in impedance were more apparent from the supernatant when the cells were removed in both cell lines. Analysis of the PC12 and either of the GBM cell line co-cultures yielded highly statistically significant differences between all comparisons of cell confluencies and frequency steps. These results illustrate that EIS can be an effective instrument for characterizing the ECM surrounding glioblastoma cells, providing insight into the cellular behavior of these oncogenic cells.
ContributorsPham, Brian (Author) / Sadleir, Rosalind (Thesis director) / Hu, Leland (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor)
Created2024-05
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Dynamic Changes in Heart Rate and Cerebral Blood Flow During Acute Vagal Nerve Stimulation

Description
Vagal Nerve Stimulation (VNS) has been shown to be a promising therapeutic technique in treating many neurological diseases, including epilepsy, stroke, traumatic brain injury, and migraine headache. The mechanisms by which VNS acts, however, are not fully understood but may involve changes in cerebral blood flow. The vagus nerve plays…

Vagal Nerve Stimulation (VNS) has been shown to be a promising therapeutic technique in treating many neurological diseases, including epilepsy, stroke, traumatic brain injury, and migraine headache. The mechanisms by which VNS acts, however, are not fully understood but may involve changes in cerebral blood flow. The vagus nerve plays a significant role in the regulation of heart rate and cerebral blood flow that are altered during VNS. Here, we examined the effects of acute vagal nerve stimulation on both heart rate and cerebral blood flow. Laser Speckle Contrast Analysis (LASCA) was used to analyze the cerebral blood flow of male Long\u2014Evans rats. Results showed two distinct patterns of responses whereby animals either experienced a mild or severe decrease in heart rate during VNS. Further, animals that displayed mild heart rate decreases showed an increase in cerebral blood flow that persisted beyond VNS. Animals that displayed severe decreases showed a transient decrease in cerebral blood flow followed by an increase that was greater than that observed in mild animals but progressively decreased after VNS. The results suggest two distinct patterns of changes in both heart rate and cerebral blood flow that may be related to the intensity of VNS.
ContributorsHillebrand, Peter Timothy (Author) / Kleim, Jeffrey (Thesis director) / Helms Tillery, Stephen (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05