Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
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- All Subjects: HPV
- Creators: School of Life Sciences
Description
Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
In the United States, a dispute has arisen over the safety and need for vaccination, particularly in regard to compulsory vaccination laws. New outlets and social media sites publish countless reports about the dangers of vaccines or of known adverse reactions as well as imagined or unproven worries. Individuals' rights to choose to get vaccinated or allow their children to be vaccinated comes to direct conflict with measures needed to protect communities from preventable viral diseases. The controversy surrounding vaccines is not new, nor necessarily are the fundamental reasons for skepticism. Looking back through the history of vaccines as a medical tool, the evolution of the controversy can be observed taking place with each new historical context, scientific development, and social conditions. Despite scientific research and assurances of vaccine safety, opposition and unease about vaccination appear to take Looking individually at the development and distribution of the smallpox (variola virus), polio (poliovirus) and human papilloma virus(HPV) vaccines, concerns regarding the violation of personal rights, safety of vaccines themselves, and social stigmas and connotations surrounding vaccines can be seen to evolve and change. Due to the way doubt can manifest in different ways over time, it may be impossible to fully end the vaccine debate. However, nderstanding the sociological factors behind anti-vaccine sentiment may allow healthcare professionals to work with concerned people with a particular care to address these visceral and sometimes irrational fears surrounding vaccination.
ContributorsStevens, Luke Christian (Author) / Jacobs, Bertram (Thesis director) / Washo-Krupps, Delon (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
Oropharyngeal cancer (OPC) is the world's sixth most common cancer and in many cases is associated with infection with human papillomavirus (HPV) type 16. Antibodies (Abs) to HPV16 viral antigens are potential diagnostic biomarkers of HPV-associated OPC (HPV OPC). A custom multiplexed bead array assay was used to detect Abs to HPV16 antigens E1, CE2, NE2, E4, E5, E6, E7, L1, and L2. Following extensive optimization of the assay, these genes were expressed as GST-fusion proteins and captured onto anti-GST magnetic beads. Serum was obtained from 256 OPC patients at the time of diagnosis and from 78 healthy controls. The median fluorescent intensity (MFI) was determined for each antigen and ratios of MFI to control GST-fusion protein were determined for each serum sample. Cutoff values were set as the mean + 3 SD of the MFIs of healthy controls and p-values were calculated using Wilcoxon unpaired and Fisher's exact test. Results of this experiment showed that HPV16 E1, CE2, NE2, E4, E6, and E7 Ab levels were elevated in OPC patients compared to controls (p<0.001), as were Ab levels to L1 (p = 0.013) and L2 (p = 0.023), per Fischer's exact test. Abs to CE2, NE2, E6, and E7 were identified as a potential biomarker panel for early detection of HPV OPC. For the 111 patients with known HPV+ tumors as measured by tumor PCR of E6 and/or E7, this assay had a sensitivity of 90% and specificity of 87% (AUC = 0.96). From these results, we conclude that custom bead array assays can be used to detect HPV16 Abs in patient sera, and we have identified a 4-Ab biomarker panel for the early detection of HPV OPC.
ContributorsGoulder, Alison Leigh (Author) / Anderson, Karen (Thesis director) / Lake, Douglas (Committee member) / Cheng, Julia (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2013-05
Description
Background: High risk types of human papillomavirus (HPV) are known to cause cancer, including cervical (99%) and oropharyngeal cancer (70%). HPV type 16 is the most common subtype. Three antigens that are critical for integration or tumor progression are E2, E6 and E7. In this study, we developed a systematic approach to identify naturally-processed HPV16-derived HLA class I epitopes for immunotherapy development. Methods: K562 cells, which lack HLA expression, were transduced with each HPV16 antigen using lentivirus and supertransfected with HLA-A2 by nucleofection. Stable cell lines expressing each antigen were selected for and maintained throughout the investigation. In order to establish a Gateway-compatible vector for robust transient gene expression, a Gateway recombination expression cloning cassette was inserted into the commercial Lonza pMAX GFP backbone, which has been experimentally shown to display high transfection expression efficiency. GFP was cloned into the vector and plain K562 cells were transfected with the plasmid by nucleofection. Results: Expression of K562-A2 was tested at various time points by flow cytometry and A2 expression was confirmed. Protein expression was shown for the transduced K562 E7 by Western blot analysis. High transfection efficiency of the pMAX_GFP_Dest vector (up to 97% GFP+ cells) was obtained 48 hours post transfection, comparable to the commercial GFP-plasmid. Conclusion: We have established a rapid system for target viral antigen co-expression with single HLA molecules for analysis of antigen presentation. Using HPV as a model system, our goal is to identify specific antigenic peptide sequences to develop immunotherapeutic treatments for HPV-associated cancers.
ContributorsVarda, Bianca Marie (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / Krishna, Sri (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Native American COVID vaccination trends have deviated from the vaccinated trends for other vaccines such as the HPV and flu. Native Americans are reporting the highest vaccinations in the nation compared to all racial and ethnic groups in the country. Despite this, some are still hesitant in taking the vaccine. This study investigates the factors that influence vaccine uptake and hesitancy among Native American Youth in Arizona. The study utilizes data from the Arizona Youth Identity Project, a mixed-methods study examining young adults’ political engagement and sense of identity as Americans. 10 out of 13 individuals at the time of the study had received the COVID vaccine. Three themes, trust, knowledge, and social responsibility were identified. Most of the reasons for hesitancy came from distrust of government and biomedicine. Most participants had no knowledge of vaccine effectiveness, mechanism or effects but viewed getting vaccinated as a social responsibility to protect their families and communities. These findings indicate that the main goal of vaccinations among Native American youth was to protect the most vulnerable elders.
ContributorsAbaidoo, Joy (Author) / Gonzales, Angela (Thesis director) / Doebbeling, Bradley (Committee member) / Barrett, The Honors College (Contributor) / Hugh Downs School of Human Communication (Contributor) / School of Life Sciences (Contributor)
Created2022-05
Description
Aims: The aim of this research is to identify factors that would lead to increased utilization of the HPV vaccine among college students.
Methods: We conducted 11 focus groups with a total of 28 students, averaging 3 per group. Using an inductive approach, we hand-coded focus group transcripts, developed a coding structure, and discussed themes as they emerged from the data.
Results: Although more than half of the students had never heard of the HPV vaccine, students generally held positive views about vaccines. Barriers to receiving the HPV vaccine included lack of awareness and knowledge about the HPV vaccine, as well as lack of perceived need for the vaccine. When asked about the most important information that they needed in order to make an informed decision about whether or not to get the vaccine, participants mentioned the following: 1) prevalence of HPV, 2) HPV-related diseases, 3) what the HPV vaccine protects against, 4) HPV vaccine safety, 5) HPV vaccine efficacy, and 6) how they can access the vaccine. Participants differed in their preferences for types of health education, from an in-person informative lecture, to YouTube videos, to posters placed in dorms or in bathrooms. They preferred the conveyor of this information to be a trustworthy source; they identified healthcare providers, professors/researchers, and other students who have received the vaccine as credible sources. In terms of message appeal, many students described wanting the facts, statistics about HPV prevalence and the vaccine as well as narratives from students who have been diagnosed with HPV and those who have experience receiving the vaccine.
Conclusions: Although this cancer prevention resource has been commercially available since 2006, college students still a lack of awareness and perceived need for the HPV vaccine. Future health education efforts should utilize participants’ recommendations to increase students’ understanding of HPV and the HPV vaccine and, therefore, impact their perceived susceptibility to HPV, the benefits of the vaccine to their health, and therefore increase utilization of this resource. Strategies to increase vaccination should include health education and vaccine implementation strategies, as well as strategies to reduce the cost of the vaccine for college students, thereby increasing the accessibility of the vaccine for this population.
Methods: We conducted 11 focus groups with a total of 28 students, averaging 3 per group. Using an inductive approach, we hand-coded focus group transcripts, developed a coding structure, and discussed themes as they emerged from the data.
Results: Although more than half of the students had never heard of the HPV vaccine, students generally held positive views about vaccines. Barriers to receiving the HPV vaccine included lack of awareness and knowledge about the HPV vaccine, as well as lack of perceived need for the vaccine. When asked about the most important information that they needed in order to make an informed decision about whether or not to get the vaccine, participants mentioned the following: 1) prevalence of HPV, 2) HPV-related diseases, 3) what the HPV vaccine protects against, 4) HPV vaccine safety, 5) HPV vaccine efficacy, and 6) how they can access the vaccine. Participants differed in their preferences for types of health education, from an in-person informative lecture, to YouTube videos, to posters placed in dorms or in bathrooms. They preferred the conveyor of this information to be a trustworthy source; they identified healthcare providers, professors/researchers, and other students who have received the vaccine as credible sources. In terms of message appeal, many students described wanting the facts, statistics about HPV prevalence and the vaccine as well as narratives from students who have been diagnosed with HPV and those who have experience receiving the vaccine.
Conclusions: Although this cancer prevention resource has been commercially available since 2006, college students still a lack of awareness and perceived need for the HPV vaccine. Future health education efforts should utilize participants’ recommendations to increase students’ understanding of HPV and the HPV vaccine and, therefore, impact their perceived susceptibility to HPV, the benefits of the vaccine to their health, and therefore increase utilization of this resource. Strategies to increase vaccination should include health education and vaccine implementation strategies, as well as strategies to reduce the cost of the vaccine for college students, thereby increasing the accessibility of the vaccine for this population.
ContributorsLi, Rina (Co-author) / Silvestro, Kenny (Co-author) / Koskan, Alexis (Thesis director) / Helitzer, Deborah (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Aims: The aim of this research is to identify factors that would lead to increased utilization of the HPV vaccine among college students.
Methods: We conducted 11 focus groups with a total of 28 students, averaging 3 per group. Using an inductive approach, we hand-coded focus group transcripts, developed a coding structure, and discussed themes as they emerged from the data.
Results: Although more than half of the students had never heard of the HPV vaccine, students generally held positive views about vaccines. Barriers to receiving the HPV vaccine included lack of awareness and knowledge about the HPV vaccine, as well as lack of perceived need for the vaccine. When asked about the most important information that they needed in order to make an informed decision about whether or not to get the vaccine, participants mentioned the following: 1) prevalence of HPV, 2) HPV-related diseases, 3) what the HPV vaccine protects against, 4) HPV vaccine safety, 5) HPV vaccine efficacy, and 6) how they can access the vaccine. Participants differed in their preferences for types of health education, from an in-person informative lecture, to YouTube videos, to posters placed in dorms or in bathrooms. They preferred the conveyor of this information to be a trustworthy source; they identified healthcare providers, professors/researchers, and other students who have received the vaccine as credible sources. In terms of message appeal, many students described wanting the facts, statistics about HPV prevalence and the vaccine as well as narratives from students who have been diagnosed with HPV and those who have experience receiving the vaccine.
Conclusions: Although this cancer prevention resource has been commercially available since 2006, college students still a lack of awareness and perceived need for the HPV vaccine. Future health education efforts should utilize participants’ recommendations to increase students’ understanding of HPV and the HPV vaccine and, therefore, impact their perceived susceptibility to HPV, the benefits of the vaccine to their health, and therefore increase utilization of this resource. Strategies to increase vaccination should include health education and vaccine implementation strategies, as well as strategies to reduce the cost of the vaccine for college students, thereby increasing the accessibility of the vaccine for this population.
Methods: We conducted 11 focus groups with a total of 28 students, averaging 3 per group. Using an inductive approach, we hand-coded focus group transcripts, developed a coding structure, and discussed themes as they emerged from the data.
Results: Although more than half of the students had never heard of the HPV vaccine, students generally held positive views about vaccines. Barriers to receiving the HPV vaccine included lack of awareness and knowledge about the HPV vaccine, as well as lack of perceived need for the vaccine. When asked about the most important information that they needed in order to make an informed decision about whether or not to get the vaccine, participants mentioned the following: 1) prevalence of HPV, 2) HPV-related diseases, 3) what the HPV vaccine protects against, 4) HPV vaccine safety, 5) HPV vaccine efficacy, and 6) how they can access the vaccine. Participants differed in their preferences for types of health education, from an in-person informative lecture, to YouTube videos, to posters placed in dorms or in bathrooms. They preferred the conveyor of this information to be a trustworthy source; they identified healthcare providers, professors/researchers, and other students who have received the vaccine as credible sources. In terms of message appeal, many students described wanting the facts, statistics about HPV prevalence and the vaccine as well as narratives from students who have been diagnosed with HPV and those who have experience receiving the vaccine.
Conclusions: Although this cancer prevention resource has been commercially available since 2006, college students still a lack of awareness and perceived need for the HPV vaccine. Future health education efforts should utilize participants’ recommendations to increase students’ understanding of HPV and the HPV vaccine and, therefore, impact their perceived susceptibility to HPV, the benefits of the vaccine to their health, and therefore increase utilization of this resource. Strategies to increase vaccination should include health education and vaccine implementation strategies, as well as strategies to reduce the cost of the vaccine for college students, thereby increasing the accessibility of the vaccine for this population.
ContributorsSilvestro, Kenneth (Co-author) / Li, Rina (Co-author) / Koskan, Alexis (Thesis director) / Helitzer, Deborah (Committee member) / Watts College of Public Service & Community Solut (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05