Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
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- All Subjects: Social Media
- All Subjects: Evolution
To determine if the disruption of the MMR pathway results in the reduced conservation of methylated adenines as well as an increased tolerance for mutations that result in the loss or gain of new GATC sites, we surveyed individual clones isolated from experimentally evolving wild-type and MMR-deficient (mutL- ;conferring an 150x increase in mutation rate) populations of E. coli with whole-genome sequencing. Initial analysis revealed a lack of mutations affecting methylation sites (GATC tetranucleotides) in wild-type clones. However, the inherent low mutation rates conferred by the wild-type background render this result inconclusive, due to a lack of statistical power, and reveal a need for a more direct measure of changes in methylation status. Thus as a first step to comparative methylomics, we benchmarked four different methylation-calling pipelines on three biological replicates of the wildtype progenitor strain for our evolved populations.
While it is understood that these methylated sites play a role in the MMR pathway, it is not fully understood the full extent of their effect on the genome. Thus the goal of this thesis was to better understand the forces which maintain the genome, specifically concerning m6A within the GATC motif.
My thesis project took the form of a networking blog for adoptable animals at the local pound. I created unique photos of the dogs and wrote up bios for them so that they became more accessible to people who could not physically visit the shelter very often. I brought the dogs to life by sharing a part of their story and encouraging people to care about what happened to them. This issue is especially close to my heart because I have loved animals all my life and started fostering dogs a few years ago.
The project was a huge success. I profiled 37 dogs and they amassed hundreds of thousands of views both on my Wordpress site and on the project Facebook page. Five of my dogs were euthanized, about a 13% euthanasia rate. Compared to the owner surrender euthanasia rate of previous years, this rate is remarkably low. In 2012, about 43.86% of owner surrenders were euthanized. In 2013, about 39.19% of owner surrenders were euthanized. In 2014, about 33.27% of owner surrenders were euthanized. My euthanasia rate was essentially less than half of the last year’s owner surrender euthanasia rate.
I think I absolutely proved my point. The power of networking in unique ways and leveraging the influence of social media cannot be underestimated. Less dogs were killed because of my project. If that’s not success, then I don’t know what is.