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Modeling SDF-1α Chemotactic Gradient Formation After Traumatic Brain Injury

Description

Traumatic brain injury (TBI) is a leading cause of injury related death in the United States. The complexity of the injury environment that follows TBI creates an incomplete understanding of all the mechanisms in place to regulate chemotactic responses to TBI. The goal of this project was to develop a…

Traumatic brain injury (TBI) is a leading cause of injury related death in the United States. The complexity of the injury environment that follows TBI creates an incomplete understanding of all the mechanisms in place to regulate chemotactic responses to TBI. The goal of this project was to develop a predictive in silco model using diffusion and autocrine/paracrine signaling specific to stromal cell derived factor-1α (SDF-1α) gradient formation after TBI and compare this model with in vivo experimental data. A COMSOL model using Fickian diffusion and autocrine/paracrine reaction terms was generated to predict the gradient formation observed in vivo at three physiologically relevant time points (1, 3, and 7 days). In vivo data was gathered and analyzed via immunohistochemistry and MATLAB. The spatial distribution of SDF-1α concentration in vivo more consistently demonstrated patterns similar to the in silico model dependent on both diffusion and autocrine/paracrine reaction terms rather than diffusion alone. The temporal distribution of these same results demonstrated degradation of SDF-1α at too rapid a rate, compared to the in vivo results. To account for differences in behavior observed in vivo, reaction terms and constants of 1st-order reaction rates must be modulated to better reflect the results observed in vivo. These results from both the in silico model and in vivo data support the hypothesis that SDF-1α gradient formation after TBI depends on more than diffusion alone. Future work will focus on improving the model with constants that are specific to SDF-1α as well as testing methods to better control the degradation of SDF-1α.

ContributorsFreeman, Sabrina Louise (Author) / Stabenfeldt, Sarah (Thesis director) / Caplan, Michael (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)

Created2016-05

A Rivalry of Roses? An analysis of the Manchester United vs. Leeds United football rivalry and the development of regional, social, and historical rivalries connected to these clubs.

Description

Manchester United and Leeds United are two of the English Premier League’s most popular and historically successful clubs, and together constitute one of English football’s most interesting and inexplicable rivalries. English popular opinion claims that this rivalry is based on the Wars of the Roses and the royal houses of…

Manchester United and Leeds United are two of the English Premier League’s most popular and historically successful clubs, and together constitute one of English football’s most interesting and inexplicable rivalries. English popular opinion claims that this rivalry is based on the Wars of the Roses and the royal houses of Lancaster and York, so this thesis engages with this idea and analyzes the rivalry's connections to this medieval historical event. Furthermore, the top flight English football league's evolution into the English Premier League brought social and economic changes to the sport, both at a broad and ground level, and this thesis finds out how much these changes affected this rivalry. All in all, this thesis analyzes medieval, social, cultural, and economic historical connections to one of English football's most unique club rivalries.

ContributorsFeyrer, Aubrey (Author) / Harper, Tobias (Thesis director) / Jackson, Victoria (Committee member) / Barrett, The Honors College (Contributor) / Historical, Philosophical & Religious Studies, Sch (Contributor)

Created2021-12