Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.

Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.

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Extensive efforts have been made to develop efficient and low-cost methods for diagnostics to identify molecular biomarkers that are linked to a wide array of conditions, including cancer. A highly developed method includes utilizing the gene-editing enzyme CRISPR-Cas12a (Cpf1), which demonstrates double-stranded DNase activity with RuvC catalytic domain with high

Extensive efforts have been made to develop efficient and low-cost methods for diagnostics to identify molecular biomarkers that are linked to a wide array of conditions, including cancer. A highly developed method includes utilizing the gene-editing enzyme CRISPR-Cas12a (Cpf1), which demonstrates double-stranded DNase activity with RuvC catalytic domain with high sensitivity and specificity. This DNase activity is RNA-guided and requires a T-rich PAM site on the target sequence for functional cleavage. There have been recent efforts to utilize this DNase activity of Cas12a by combining it with isothermal amplification and analysis by lateral strip tests. This project examined CRISPR-based early detection of microRNA biomarkers. MicroRNA are short RNA molecules that have large roles in post-transcriptional gene regulation. However, due the short length of microRNA and its single-stranded nature, it is challenging to use Cas12a for microRNA detection using existing methods. Thus, this project investigated the potential of two microRNA detection strategies for recognition by CRISPR-Cas12a. These methods were microRNA-splinted ligation with polymerase chain reaction (PCR) and MicroRNA-specific reverse transcriptase PCR (RT-PCR). Gel imaging demonstrated effective amplification of ligated DNA through microRNA-splinted ligation with PCR/RPA. In addition, lateral strips tests showed effective cleavage of the target sequences by Cas12a. However, RT-PCR method demonstrated low amplification by PCR and inefficient poly(A) elongation. This project paves the way for the detection of an extensive range of microRNA biomarkers that are linked to an array of diseases. Future directions include analysis and modifications of RT-PCR method to improve experimental results, extending these detection methods to a larger range of microRNA sequences, and eventually utilizing them for detection in human samples.
ContributorsStaren, Michael Steven (Author) / Green, Alexander (Thesis director) / Stephanopoulos, Nicholas (Committee member) / Diehnelt, Chris (Committee member) / School of Life Sciences (Contributor) / College of Health Solutions (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description

This paper explores the well-known Atkins Diet, as it also places a strong regulation on macromolecule consumption, specifically carbohydrates, in order to assist with the weight loss process. A review of available literature will be used to investigate: the history of the diet, necessity of macromolecule consumption, the impact this

This paper explores the well-known Atkins Diet, as it also places a strong regulation on macromolecule consumption, specifically carbohydrates, in order to assist with the weight loss process. A review of available literature will be used to investigate: the history of the diet, necessity of macromolecule consumption, the impact this has on the individual biochemical pathways (glycolysis/gluconeogenesis) and the microbiome as a whole, as well as overall success rates and long-term health complications/benefits. Additionally personal statements from various individuals who have experience with the diet, myself included, will be incorporated into a holistic analysis of the effectiveness and longevity of the Atkins weight-loss strategy.

ContributorsButler, Jessica Carol (Author) / Sellner, Erin (Thesis director) / Gray, Tiffany (Committee member) / College of Health Solutions (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Food is one of the most universal and uniting human experiences. It is a powerful tool to bring communities together and it is a simple way to bring joy to an individual. This project is an exercise in marketing and entrepreneurship that was inspired by these ideas, which culminated in

Food is one of the most universal and uniting human experiences. It is a powerful tool to bring communities together and it is a simple way to bring joy to an individual. This project is an exercise in marketing and entrepreneurship that was inspired by these ideas, which culminated in a fundraiser bake sale to benefit Creighton Community Foundation, a local nonprofit.

ContributorsLondono, Jane (Author) / Byrne, Jared (Thesis director) / Martinelli, Sarah (Committee member) / College of Health Solutions (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2022-05
Description

In January of 2022, 61 women from Afghanistan were accepted to Arizona State University and joined our campus from the Asian University of Women. Of One Heart is a Phoenix based nonprofit which aims to connect refugees with mentors to cultivate intercultural relationships, assist refugees in integrating into a new

In January of 2022, 61 women from Afghanistan were accepted to Arizona State University and joined our campus from the Asian University of Women. Of One Heart is a Phoenix based nonprofit which aims to connect refugees with mentors to cultivate intercultural relationships, assist refugees in integrating into a new community, and to empower refugees to utilize their unique perspectives and talents in their new home. In addition to these goals, these relationships aim to connect refugees with the networks their mentor has and to assist them in understanding the new systems and norms of American culture. The relationship is reciprocal in the sharing of background and stories to facilitate trust and to recognize the value refugees have to contribute to society. The mission of this project is to implement the Of One Heart mentoring model onto ASU campus to help facilitate intercultural friendships between our new students from Afghanistan and other ASU students, faculty and staff. In doing so, we hope to create a model demonstrating refugee student success by collecting data through pre and post program surveys to track if involvement in the program improved participants utilization of existing ASU resources, cultural competency, mental health, and participation in community activities and internships/job opportunities. Ideally, we hope to create a program model which is proven to support refugee students to be replicated for future semesters as the program expands to serve not only the students from Afghanistan, but all refugee and asylum seeking students.

ContributorsPernat, Caroline (Author) / Gutierrez, Veronica (Thesis director) / Baldwin, Kimberly (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor) / School of Public Affairs (Contributor) / College of Integrative Sciences and Arts (Contributor)
Created2023-05