Barrett, The Honors College Thesis/Creative Project Collection
Barrett, The Honors College at Arizona State University proudly showcases the work of undergraduate honors students by sharing this collection exclusively with the ASU community.
Barrett accepts high performing, academically engaged undergraduate students and works with them in collaboration with all of the other academic units at Arizona State University. All Barrett students complete a thesis or creative project which is an opportunity to explore an intellectual interest and produce an original piece of scholarly research. The thesis or creative project is supervised and defended in front of a faculty committee. Students are able to engage with professors who are nationally recognized in their fields and committed to working with honors students. Completing a Barrett thesis or creative project is an opportunity for undergraduate honors students to contribute to the ASU academic community in a meaningful way.
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- All Subjects: conservation
- Creators: Ames, Amanda Gail
- Creators: Barton, Madisen L
Description
White-nose syndrome (WNS) is a fungal infection devastating bat populations throughout eastern North America. WNS is caused by a fungus, Pseudogymnoascus destructans (Pd), that invades the skin of hibernating bats. While there are a number of treatments being researched, there is currently no effective treatment for WNS that is deployed in the field, except a few being tested on a limited scale. Bats have lowered immune function and response during hibernation, which may increase susceptibility to infection during the winter months. Antimicrobial peptides (AMPs) are a crucial component of the innate immune system and serve as barriers against infection. AMPs are constitutively expressed on skin and facilitate wound healing, stimulate other immune responses, and may also stay active on bat skin during hibernation. AMPs are expressed by all tissues, have direct killing abilities against microbes, and are a potential treatment for bats infected with Pd. In this investigation, the fungicidal activity of several readily available commercial AMPs were compared, and killing assay protocols previously investigated by Frasier and Lake were replicated to establish a control trial for use in future killing assays. Another aim of this investigation was to synthesize a bat-derived AMP for use in the killing assay. Sequences of bat-derived AMPs have been identified in bat skin samples obtained from a large geographic sampling of susceptible and resistant species. Contact was made with GenScript Inc., the company from which commercially available AMPs were purchased, to determine the characteristics of peptide sequences needed to synthesize an AMP for lab use. Based on recommendations from GenScript Inc., peptide sequences need to have a hydrophobicity of less than 50% and a sequence length of less than 50 amino acids. These criteria serve as a potential barrier because none of the known bat-derived sequences analyzed satisfy both of these requirements. The final aim of this study was to generate a conceptual model of the immune response molecules activated when bats are exposed to a fungal pathogen such as Pd. Overall, this work investigated sources of variability between trials of the killing assay, analyzed known bat-derived peptide sequences, and generated a conceptual model that will serve as a guideline for identification of immune response molecules on the skin of bats in future proteomics work.
ContributorsBarton, Madisen L (Author) / Moore, Marianne (Thesis director) / Penton, Christopher (Committee member) / College of Integrative Sciences and Arts (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Southern Arizona was once described as a "sea of grass" extending across the four major valleys, the Sulphur Spring Valley, the San Pedro Valley, the San Simon Valley and the San Bernardino Valley. But today the majority of that land is covered with desert shrubs like mesquite, leaving little to none of the natural grasses that once dominated these valleys. By the late 1800s Americans were flocking to southern Arizona to take advantage of some of the lushest grasslands the United States had to offer. Yet today we can find very little of these grasslands remaining, and so the image of this once productive land has been long forgotten. This thesis/creative project takes an in-depth look at what the land in Cochise County, Arizona once was, what it has become, and what happened to cause these drastic changes. It looks at the four major theories as to what caused these changes. The first of which is the overgrazing of cattle through the cattle boom of the late 1800s. The second is the effect of climactic events like drought and an increase in aridity over time. The third is the encroachment of what was thought to be non-native mesquite, which choked out the natural grasses. And the fourth and final theory is that the overarching suppression of fire by settlers allowed desert shrubs to expand their ranges into the grasslands. Through historical records like newspaper articles, photo archives, land surveys, military travel journals, census data, weather records as well as prior research works and interviews with researchers, conservationists and ranchers, a history of these lands is presented to show the major turning points in the lands' use and determine what led to their deterioration.
ContributorsAmes, Amanda Gail (Author) / Minteer, Ben (Thesis director) / Klett, Mark (Committee member) / Rowe, Helen (Committee member) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12