Matching Items (2)
Description
Parkinson's disease (PD) is the second most common neurodegenerative disease and is characterized by impaired motor function including tremor, rigidity, akinesia and postural instability as well as non-motor symptoms including cognitive impairment, depression, and anxiety. Pathological indices of PD consist of protein aggregation of α-synuclein (ɑ-syn), striatal dopaminergic denervation and the loss of dopaminergic neurons in the substantia nigra. While the exact causes of PD remain unknown, substantial evidence suggests that the
kynurenine pathway (KP), a pathway upregulated in response to inflammation, is implicated in its pathology. Under normal physiological conditions, the KP catabolizes the amino acid tryptophan to the metabolite 2-amino-3-carboxymuconate-6-semialdehyde (ACMS). ACMS is then spontaneously converted to quinolinic acid (QA), which is an intermediate in the formation of nicotinamide adenine dinucleotide (NAD+), a cofactor in mitochondrial respiration, thus serving as an essential molecule in cellular energy metabolism. QA itself may serve as a contributing factor in PD pathology as this metabolite promotes oxidative stress, mitochondrial dysfunction, excitotoxicity, and positively correlates with increased scores in the Unified Parkinson’s Disease Rating Scale (UPDRS) in motor experiences of daily living (UPDRS II) and motor complications (UPDRS III). ACMS is enzymatically converted to picolinic acid (PA), an iron chelator and anti-viral agent, by 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase (ACMSD), thus limiting the formation of QA and potentially conferring neuroprotective effects. To model Parkinson’s disease, we overexpressed α-synuclein unilaterally in the substantia nigra with an adeno-associated virus (AAV), which leads to dose-dependent neurodegeneration, aggregation of α-syn and motor impairments. This model may therefore serve to examine perturbations of the KP in the context of PD including increased QA/PA expression. We hypothesize that the overexpression of the enzyme ACMSD in the AAV-α-syn model of PD will reduce the degree of neurodegeneration and inflammation and prevent motor decline. Neurodegeneration and neuroinflammation will be quantified histologically, through stereology and densitometry respectively. Observable PD-like symptoms will be quantified using behavioral assessments, particularly the cylinder test and amphetamine induced rotations to assess the degree of motor decline.
ContributorsCaruso, Anthony (Author) / Manfredsson, Fredric (Thesis director) / Meyers, Kimberly (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2024-05
Description
Schizophrenia, a debilitating neuropsychiatric disorder, affects 1% of the population. This multifaceted disorder is comprised of positive (hallucinations/psychosis), negative (social withdrawal/anhedonia) and cognitive symptoms. While treatments for schizophrenia have advanced over the past few years, high economic burdens are still conferred to society, totaling more than $34 billion in direct annual costs to the United States of America. Thus, a critical need exists to identify the factors that contribute towards the etiology of schizophrenia. This research aimed to determine the interactions between environmental factors and genetics in the etiology of schizophrenia. Specifically, this research shows that the immediate early gene, early growth response 3 (EGR3), which is upregulated in response to neuronal activity, resides at the center of a biological pathway to confer risk for schizophrenia. While schizophrenia-risk proteins including neuregulin 1 (NRG1) and N-methyl-D-aspartate receptors (NMDAR’s) have been identified upstream of EGR3, the downstream targets of EGR3 remain relatively unknown. This research demonstrates that early growth response 3 regulates the expression of the serotonin 2A-receptor (5HT2AR) in the frontal cortex following the physiologic stimulus, sleep deprivation. This effect is translated to the level of protein as 8 hours of sleep-deprivation results in the upregulation of 5HT2ARs, a target of antipsychotic medications. Additional downstream targets were identified following maximal upregulation of EGR3 through electroconvulsive stimulation (ECS). Both brain-derived neurotrophic factor (BDNF) and its epigenetic regulator, growth arrest DNA-damage-inducible 45 beta (GADD45B) are upregulated one-hour following ECS in the hippocampus and require the presence of EGR3. These proteins play important roles in both cellular proliferation and dendritic structural changes. Next, the effects of ECS on downstream neurobiological processes, hippocampal cellular proliferation and dendritic structural changes were examined. Following ECS, hippocampal cellular proliferationwas increased, and dendritic structural changes were observed in both wild-type and early growth response 3 knock-out (Egr3-/-) mice. Effects in the number of dendritic spines and dendritic complexity following ECS were not found to require EGR3. Collectively, these results demonstrate that neuronal activity leads to the regulation of schizophrenia risk proteins by EGR3 and point to a possible molecular mechanism contributing risk for schizophrenia.
ContributorsMeyers, Kimberly (Author) / Gallitano, Amelia L (Thesis advisor) / Newbern, Jason (Thesis advisor) / Mangone, Marco (Committee member) / Nikulina, Ella (Committee member) / Qiu, Shenfeng (Committee member) / Ferguson, Deveroux (Committee member) / Arizona State University (Publisher)
Created2020