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- Member of: ASU Electronic Theses and Dissertations
Description
Embryonic and juvenile development consist of a series of complex and rapid changes driven by a suite of crucially timed developmental cues within the cell. The developmental process begins at the moment of zygote activation, “jump-started” by maternal factors such as mRNA and proteins until transcription can be zygotically-driven. Regulation of transcription initiation plays a crucial role in this process, as minute changes in the timing, density, and characteristics of gene expression can have drastic effects on the zygote’s development. Specific promoter elements can be linked to different patterns of transcription, driving both ubiquitous and sharply regulated gene expression, thus forming the basis for the time-sensitive developmental processes. In order to better understand the genes expressed during different stages of development and the impact of promoter elements on transcription patterns and transcript concentrations within the cell, I created a Gene Expression and Promoter Atlas in two species within the cryptic species complex, Daphnia pulex. I surveyed five embryonic and two juvenile developmental stages in both a North American and mitochondrially European Daphnia pulex utilizing developmental landmarks to visually stages embryos. A total of 17,993 genes were identified in the European species and 15,295 were identified in the North American species, with 11,551 orthologs identified between the two. I utilized the transcription start site (TSS) profiling method STRIPE-seq to identify promoter motifs and RNA-seq to survey mRNA concentration at each stage, generating a wealth of genetic data. The methodology for library construction and the dataset generated therein provide an informative basis for further comparative developmental studies and the elucidation of full gene functionality in an emerging model organism.
ContributorsWalls, Sarah (Author) / Lynch, Michael (Thesis advisor) / Raborn, R. Taylor (Committee member) / Mangoni, Marco (Committee member) / Harris, Robin (Committee member) / Arizona State University (Publisher)
Created2022
Description
This research project introduces the Czech composer Miloslav Gajdoš (b. 1948) to classical guitarists through his composition Prelude and Fugue in A Minor, composed in 1998. Gajdoš is a double bass virtuoso who has enjoyed a successful career performing, composing, and teaching. After the fall of the communist regime in Czechoslovakia in 1989, Gajdoš was allowed more opportunities to perform outside the Czech Republic and to become better known throughout the world. His Prelude and Fugue in A Minor, originally for solo double bass, works well on the guitar and is a rewarding piece to learn and perform. A transcription is presented here that is of publishable quality, together with a biography of Gajdoš and a performance guide. The biography was written from available research materials as well as from direct email correspondence with the composer, and includes authorized quotations from those emails. This project also includes a description of the piece together with musical and technical suggestions that will aid the performer in creating a satisfying musical interpretation. Chapter Three includes a description of the left-hand challenges that were encountered while the piece was being transcribed and the solutions that were devised to mitigate them. Finding new pieces to transcribe for the guitar has long been an important activity of serious players, and this transcription adds a substantial and expressive piece to the growing repertoire of the classical guitar.
ContributorsPrillaman, Aaron Paul (Author) / Koonce, Frank (Thesis advisor) / Holbrook, Amy (Committee member) / Rotaru, Catalin (Committee member) / Arizona State University (Publisher)
Created2017