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Type I H+-PPase encoding genes, such as AVP1 (Arabidopsis thaliana), TsVP (Thellungiella halophilla), TaVP,( Triticum aestivum), and OVP1 (Oryza sativa) are highly conserved and.traditionally known to operate as vacuolar proton translocating pyrophosphatases. It is worth mentioning that Rocha-Facanha and de Meis presented in vitro evidence with tonoplast fractions of maize coleoptiles and seeds consistent with the reverse function of the H+-PPase (1998). These authors suggested that given the appropriate thermodynamic conditions in vivo, the H+-PPase could operate as a system of energy conservation with a role in the maintenance of cytosolic PPi levels. Further evidence in support for a PPi-synthase activity of plant H+-PPases came from work done on tonoplasts from mature oranges where PPi synthesis was demonstrated when a ΔpH of 3 units was imposed (Marsh et al. 2000).
Futher research has shown that transgenics overexpressing type I H+-PPases develop more root and shoot biomass, and have enhanced rhizosphere acidification capacity than wild types. The increased root biomass suggests that previous reports describing the response of these plants to water scarcity as drought tolerance are incomplete. Larger root systems indicate that an important component of the response is drought resistance. The enhanced rhizosphere acidification capacity has also been associated with an increase in nutrient use efficiency, conferring a growth advantage under nitrogen and phosphorous deficient conditions.
While a vacuolar localized H+-PPase easily explains the salt tolerant phenotypes, it does little to provide a mechanism for an increase in root and shoot biomass and/or an augmented rhizosphere acidification capacity. Several groups have argued that higher levels and transport of the growth hormone auxin could be responsible for the above phenotypes. An alternative model focusing on the function of a plasma membrane bound H+-PPase in sieve elements and companion cells links these phenotypes with enhanced phloem sucrose loading and transport.
The following paper reviews publications in which the H+-PPase overexpression technology has been used since 2006 in an attempt to identify cues that could help us test the compatibility of the the proposed models with the actual data.
Futher research has shown that transgenics overexpressing type I H+-PPases develop more root and shoot biomass, and have enhanced rhizosphere acidification capacity than wild types. The increased root biomass suggests that previous reports describing the response of these plants to water scarcity as drought tolerance are incomplete. Larger root systems indicate that an important component of the response is drought resistance. The enhanced rhizosphere acidification capacity has also been associated with an increase in nutrient use efficiency, conferring a growth advantage under nitrogen and phosphorous deficient conditions.
While a vacuolar localized H+-PPase easily explains the salt tolerant phenotypes, it does little to provide a mechanism for an increase in root and shoot biomass and/or an augmented rhizosphere acidification capacity. Several groups have argued that higher levels and transport of the growth hormone auxin could be responsible for the above phenotypes. An alternative model focusing on the function of a plasma membrane bound H+-PPase in sieve elements and companion cells links these phenotypes with enhanced phloem sucrose loading and transport.
The following paper reviews publications in which the H+-PPase overexpression technology has been used since 2006 in an attempt to identify cues that could help us test the compatibility of the the proposed models with the actual data.
ContributorsCoulter, Joshua (Author) / Gaxiola, Roberto (Thesis director) / Wojciechowski, Martin (Committee member) / Pizzio, Gaston (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2014-05