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Description
The FoF1 ATP synthase is a molecular motor critical to the metabolism of virtually all life forms, and it acts in the manner of a hydroelectric generator. The F1 complex contains an (αβ)3 (hexamer) ring in which catalysis occurs, as well as a rotor comprised by subunit-ε in addition to

The FoF1 ATP synthase is a molecular motor critical to the metabolism of virtually all life forms, and it acts in the manner of a hydroelectric generator. The F1 complex contains an (αβ)3 (hexamer) ring in which catalysis occurs, as well as a rotor comprised by subunit-ε in addition to the coiled-coil and globular foot domains of subunit-γ. The F1 complex can hydrolyze ATP in vitro in a manner that drives counterclockwise (CCW) rotation, in 120° power strokes, as viewed from the positive side of the membrane. The power strokes that occur in ≈ 300 μsec are separated by catalytic dwells that occur on a msec time scale. A single-molecule rotation assay that uses the intensity of polarized light, scattered from a 75 × 35 nm gold nanorod, determined the average rotational velocity of the power stroke (ω, in degrees/ms) as a function of the rotational position of the rotor (θ, in degrees, measured in reference to the catalytic dwell). The velocity is not constant but rather accelerates and decelerates in two Phases. Phase-1 (0° - 60°) is believed to derive power from elastic energy in the protein. At concentrations of ATP that limit the rate of ATP hydrolysis, the rotor can stop for an ATP-binding dwell during Phase-1. Although the most probable position that the ATP-binding dwell occurs is 40° after the catalytic dwell, the ATP-binding dwell can occur at any rotational position during Phase-1 of the power stroke. Phase-2 of the power stroke (60° - 120°) is believed to be powered by the ATP-binding induced closure of the lever domain of a β-subunit (as it acts as a cam shaft against the γ-subunit). Algorithms were written, to sort and analyze F1-ATPase power strokes, to determine the average rotational velocity profile of power strokes as a function of the rotational position at which the ATP-binding dwell occurs (θATP-bd), and when the ATP-binding dwell is absent. Sorting individual ω(θ) curves, as a function of θATP-bd, revealed that a dependence of ω on
θATP-bd exists. The ATP-binding dwell can occur even at saturating ATP concentrations. We report that ω follows a distinct pattern in the vicinity of the ATP-binding dwell, and that the ω(θ) curve contains the same oscillations within it regardless of θATP-bd. We observed that an acceleration/deceleration dependence before and after the ATP-binding dwell, respectively, remained for increasing time intervals as the dwell occurred later in Phase-1, to a maximum of ≈ 40°. The results were interpreted in terms of a model in which the ATP-binding dwell results from internal drag at a variable position on the γε rotor.
ContributorsBukhari, Zain Aziz (Author) / Frasch, Wayne D. (Thesis director) / Allen, James P. (Committee member) / Redding, Kevin (Committee member) / School of Molecular Sciences (Contributor) / Department of Physics (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description

Sports analytics refers to the implementation of data science and analytics techniques within the sports industry. Several sports analysts and team managers have utilized analytical tools to boost overall team and player performance, often through the analysis of historical data. One of the most common techniques employed in sports analytics

Sports analytics refers to the implementation of data science and analytics techniques within the sports industry. Several sports analysts and team managers have utilized analytical tools to boost overall team and player performance, often through the analysis of historical data. One of the most common techniques employed in sports analytics is that of data mining–the extensive practice of analyzing data in order to extract and deliver insights and findings. Data mining projects are frequently guided with the six-step Cross Industry Standard Process for Data Mining (CRISP-DM) framework. One such sport that has extensively used data science and analytics, and data mining specifically, is that of Formula One (F1). Given the sports’ reliance on technology, race engineers working for F1 constructors often develop statistical models analyzing historical race performance to derive insight of drivers’ success. For the purposes of this project, the perspective of a race engineer working for the F1 constructor McLaren was considered. As the constructor is seeking to gain a competitive advantage for the upcoming F1 season, race performance data concerning previous seasons was collected and analyzed as part of a larger data mining project utilizing the CRISP-DM framework. Statistical models, such as linear regression and random forest, were developed to predict the number of points scored by McLaren racers and the variables most strongly contributed to such scored points. The final results point to specific lap times having to be aimed for as the most important variable in determining the number of points gained, although specific locations also seem prone to McLaren race success. These results in turn will be utilized to develop race strategies for the upcoming season to ensure McLaren has high efficiency against its competitors.

ContributorsImam, Amir (Author) / Simon, Alan (Thesis director) / Sha, Xiqing (Committee member) / Barrett, The Honors College (Contributor) / Department of Information Systems (Contributor)
Created2023-05