Matching Items (3)

Acute tau knockdown in the hippocampus of adult mice causes learning and memory deficits

Description
To date, it has been difficult to elucidate the role of tau in learning and memory during adulthood due to developmental compensation of other microtubule associated proteins in Tau knockout (KO) mice. Here, we generated an adeno-associated virus (AAV) expressing a doxycycline (doxy)-inducible short-hairpin (sh) RNA targeted to tau, and…

To date, it has been difficult to elucidate the role of tau in learning and memory during adulthood due to developmental compensation of other microtubule associated proteins in Tau knockout (KO) mice. Here, we generated an adeno-associated virus (AAV) expressing a doxycycline (doxy)-inducible short-hairpin (sh) RNA targeted to tau, and stereotaxically and bilaterally injected 7-month-old C57BL/6 mice with either the AAV-shRNAtau or an AAV expressing a scramble shRNA sequence. Seven days after the injections, all animals were administered doxy for thirty-five days to induce expression of shRNAs, after which they were tested in the open field, rotarod and Morris water maze (MWM) to assess anxiety like behavior, motor coordination and spatial reference memory, respectively. Our results show that reducing tau in the adult hippocampus produces significant impairments in motor coordination, endurance and spatial memory. Tissue analyses shows that tau knockdown reduces hippocampal dendritic spine density and the levels of BDNF and synaptophysin, two proteins involved in memory formation and plasticity. Our approach circumvents the developmental compensation issues observed in Tau KO models and shows that reducing tau levels during adulthood impairs cognition.
ContributorsTran, An Le (Author) / Oddo, Salvatore (Thesis director) / Velazquez, Ramon (Committee member) / Roberson, Erik (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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Crypto for Corporations: Industry Transformation Through NFT

Description
The use of cryptocurrencies is on the rise and Non-Fungible Tokens (NFTs) are transforming the way in which businesses conduct their daily practices, as well as changing the traditional view of assets. As NFTs are relatively new, the research is not fully inclusive of all of the business applications.…

The use of cryptocurrencies is on the rise and Non-Fungible Tokens (NFTs) are transforming the way in which businesses conduct their daily practices, as well as changing the traditional view of assets. As NFTs are relatively new, the research is not fully inclusive of all of the business applications. This paper seeks to expand upon the current research by determining if NFTs are a practical and accessible tool in businesses. We seek to explore innovative ways to tokenize artwork as well as market opportunities for artwork NFTs. After a comprehensive literature review, an interview with renowned comic book author, Todd McFarlane, was conducted to explore his experience with NFTs. In order to achieve an immersive experience, we created our own NFTs and brand. Our NFT storefront is hosted on OpenSea and an Instagram marketing campaign was used for advertising. After launching two NFT collections, a total of 4 NFTs were sold and the Instagram account grew to over 800 followers. We have determined that the increased popularity of NFTs has led to an increase in accessibility and practicality, making NFTs a viable business tool.
ContributorsKehoe, Amy (Author) / Pumel, Alexis (Co-author) / Zhang, Zhongju (Thesis director) / Wang, Jiaxu (Committee member) / Barrett, The Honors College (Contributor) / Department of Economics (Contributor) / Department of Information Systems (Contributor)
Created2022-05
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Crypto for Corporations: Industry Transformation Through NFT

Description

The use of cryptocurrencies is on the rise and Non-Fungible Tokens (NFTs) are transforming the way in which businesses conduct their daily practices, as well as changing the traditional view of assets. As NFTs are relatively new, the research is not fully inclusive of all of the business applications. This…

The use of cryptocurrencies is on the rise and Non-Fungible Tokens (NFTs) are transforming the way in which businesses conduct their daily practices, as well as changing the traditional view of assets. As NFTs are relatively new, the research is not fully inclusive of all of the business applications. This paper seeks to expand upon the current research by determining if NFTs are a practical and accessible tool in businesses. We seek to explore innovative ways to tokenize artwork as well as market opportunities for artwork NFTs. After a comprehensive literature review, an interview with renowned comic book author, Todd McFarlane, was conducted to explore his experience with NFTs. In order to achieve an immersive experience, we created our own NFTs and brand. Our NFT storefront is hosted on OpenSea and an Instagram marketing campaign was used for advertising. After launching two NFT collections, a total of 4 NFTs were sold and the Instagram account grew to over 800 followers. We have determined that the increased popularity of NFTs has led to an increase in accessibility and practicality, making NFTs a viable business tool.
ContributorsPumel, Alexis (Author) / Kehoe, Amy (Co-author) / Zhang, Zhongji (Thesis director) / Wang, Jiaxu (Committee member) / Barrett, The Honors College (Contributor) / Department of Marketing (Contributor) / Department of Information Systems (Contributor)
Created2022-05