Jaguar population decline is largely attributed to habitat loss and retaliatory hunting. Maintaining a viable prey base in the wild can help to mitigate this issue and decrease human-wildlife conflicts. This study aims to assess the presence of prey species in jaguar habitat in order to inform conservation efforts to maintain and improve the health and relative abundance of the wildlife community. We analyzed nearly 40,000 photographs from 85 camera traps to assess the presence of prey species at sites where jaguars are known to occur. Jaguar-prey site overlap was calculated based on the percent of jaguar sites where each prey species was present. Medium-sized mammal prey species (e.g. Central American agouti) were present across the majority (up to 87%) of sites, while large mammal prey species were present in 16%-42% of sites, varying by species. These results suggest that conservation management of jaguars would benefit from improved monitoring and maintenance of a stable prey community.