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HIV/AIDS is the sixth leading cause of death worldwide and the leading cause of death among women of reproductive age living in low-income countries. Clinicians in industrialized nations monitor the efficacy of antiretroviral drugs and HIV disease progression with the HIV-1 viral load assay, which measures the copy number of

HIV/AIDS is the sixth leading cause of death worldwide and the leading cause of death among women of reproductive age living in low-income countries. Clinicians in industrialized nations monitor the efficacy of antiretroviral drugs and HIV disease progression with the HIV-1 viral load assay, which measures the copy number of HIV-1 RNA in blood. However, viral load assays are not widely available in sub-Saharan Africa and cost between 50-$139 USD per test on average where available. To address this problem, a mixed-methods approach was undertaken to design a novel and inexpensive viral load diagnostic for HIV-1 and to evaluate barriers to its adoption in a developing country. The assay was produced based on loop-mediated isothermal amplification (LAMP). Blood samples from twenty-one individuals were spiked with varying concentrations of HIV-1 RNA to evaluate the sensitivity and specificity of LAMP. Under isothermal conditions, LAMP was performed with an initial reverse-transcription step (RT-LAMP) and primers designed for HIV-1 subtype C. Each reaction generated up to a few billion copies of target DNA within an hour. Presence of target was detected through naked-eye observation of a fluorescent indicator and verified by DNA gel electrophoresis and real-time fluorescence. The assay successfully detected the presence of HIV in samples with a broad range of HIV RNA concentration, from over 120,000 copies/reaction to 120 copies/reaction. In order to better understand barriers to adoption of LAMP in developing countries, a feasibility study was undertaken in Tanzania, a low-income country facing significant problems in healthcare. Medical professionals in Northern Tanzania were surveyed for feedback regarding perspectives of current HIV assays, patient treatment strategies, availability of treatment, treatment priorities, HIV transmission, and barriers to adoption of the HIV-1 LAMP assay. The majority of medical providers surveyed indicated that the proposed LAMP assay is too expensive for their patient populations. Significant gender differences were observed in response to some survey questions. Female medical providers were more likely to cite stigma as a source problem of the HIV epidemic than male medical providers while males were more likely to cite lack of education as a source problem than female medical providers.
ContributorsSalamone, Damien Thomas (Author) / Jacobs, Bertram L (Thesis advisor) / Marsiglia, Flavio (Committee member) / Stout, Valerie (Committee member) / Johnson, Crista (Committee member) / Arizona State University (Publisher)
Created2011
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This paper will focus on the changes in China's OFDI while also explaining its growth. However, another primary focus will be comparing the relationships between China, Hong Kong, and Africa. This paper will show the correlating changes between the three regions and explain the distribution of China's investments. One argument

This paper will focus on the changes in China's OFDI while also explaining its growth. However, another primary focus will be comparing the relationships between China, Hong Kong, and Africa. This paper will show the correlating changes between the three regions and explain the distribution of China's investments. One argument is that Hong Kong may play a large role in facilitating Chinese investment into Africa, which if not disaggregated, could lead to inaccurate numbers of China's FDI into Africa. The purpose of this paper is to investigate the importance of China's relationship with Hong Kong and Africa. In 2012, Garth Shelton argued that Hong Kong was an important gateway in South Africa's trade with China. Since then, many others have made similar claims in support of Hong Kong's bigger role. However, due to the difficulty of finding specific data for each region, these analyses are incomplete and fail to clearly substantiate their theory. I will try to find a correlation by gathering my own data, tables, and through different interviews.
ContributorsSon, James (Author) / Simonson, Mark (Thesis director) / Iheduru, Okechukwu (Committee member) / Economics Program in CLAS (Contributor) / Department of Finance (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description

The deadly shipwrecks of migrant boats in the Mediterranean brought international attention to the plight of migrants in the mid-2010s but the focus soon shifted from humanitarian assistance to capturing smugglers and preventing migrants from reaching the shores of Europe. The step towards a humane migration policy was a short-lived

The deadly shipwrecks of migrant boats in the Mediterranean brought international attention to the plight of migrants in the mid-2010s but the focus soon shifted from humanitarian assistance to capturing smugglers and preventing migrants from reaching the shores of Europe. The step towards a humane migration policy was a short-lived diversion from the project of “Fortress Europe” undertaken since the passing of the Schengen Convention. This project seeks to harden the external borders of Europe and prevent refugees from accessing the asylum system by enlisting neighboring non-European states to prevent migration at the point of departure. Deals such as the EU-Turkey deal of 2016 and the Spanish-Moroccan deals have resulted in migrants being funneled into increasingly dangerous corridors, such as Libya, as the safest and shortest paths are cut off. Although these deals are problematic in their own right, they pale in comparison to the egregious Italy-Libya Memorandum of 2017, which in practice enables Libyan militias to enforce Italy’s migration policy within the Libyan “rescue zone.” The human rights abuses perpetrated by these Libyan mercenaries in makeshift detention centers and on the Mediterranean are well documented, yet the Italian government continues to renew the deal and continue supplying these criminal groups. This literature review examines the issue of European border externalization in the Mediterranean and its impact on the internationally recognized rights of migrants and the stability of African governments. Using a systematic review of existing research, I analyze the key themes and trends that have emerged in the literature on this topic, including the legal and ethical implications of border externalization policies, the impact on African economies and governments, and the human rights implications for migrants. The review concludes that international courts are becoming increasingly ineffective in enforcing the rights of refugees and recommends a reform of the international refugee protection regime to favor autonomous movement.

ContributorsYousefelahi, Shawn (Author) / Wheatley, Abby (Thesis director) / Ripley, Charles (Committee member) / Paynter, Eleanor (Committee member) / Barrett, The Honors College (Contributor) / Economics Program in CLAS (Contributor)
Created2023-05