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- Creators: Newbern, Jason
Description
Autism spectrum disorder (ASD) is characterized by deficits in flexible cognition and social behavior. The most common atypical brain structure in ASD, the cerebellum, has multisynaptic connections through the cerebellar nuclei (CN) and thalamus to cognitive- and social-associated brain regions, yet formation and modulation of these pathways are not fully understood. Additionally, a CN output mechanism, perineuronal nets (PNNs), structure and function are undefined. PNNs are specialized extracellular matrix structures whose appearance is associated with the end of the critical period of plasticity and have been implicated in learning and neurodevelopmental disorders, but their role in the CN during development is unknown.To examine the role of CN on cognition, CN activity was increased or decreased in both male and female mice using Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) from postnatal day 21-35. Learning and reversal was analyzed using a pairwise visual discrimination task. Social behavior was assessed using a classic three-chamber assay and analyzed using SLEAP (Social Leap Estimates Animal Poses). A marker of critical periods, perineuronal nets (PNNs), was examined to understand relationships between neural development and behavior.
Interestingly, adolescent CN disruption did not alter task acquisition, yet correct choice reversal performance was dependent on DREADD manipulation and sex. CN inhibition improved reversal learning in males (5 days faster to criteria) and CN excitation improved female reversal learning (10 days faster to criteria) compared to controls. Analysis of social behavior revealed male social preference was abolished in CN manipulated groups, whereas females failed to demonstrate a social preference. Interestingly, CN manipulation in females regardless of direction, reduced PNN intensity, whereas in males only CN inhibition reduced PNN intensity. PNN intensity negatively correlated with reversal performance. CN PNN intensity showed no relation to social behavior. These data suggest chronic adolescent CN manipulation may have compensatory changes in PNN structure and CN output to improve reversal learning and PNN function was unrelated to social behavior. This study provides new evidence for CN in non-motor functions and sex-dependent differences in behavior and CN plasticity.
ContributorsLyle, Tristan (Author) / Verpeut, Jessica (Thesis advisor) / Sanabria, Federico (Committee member) / Newbern, Jason (Committee member) / Arizona State University (Publisher)
Created2024