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In oxygenic photosynthesis, conversion of solar energy to chemical energy is catalyzed by the<br/>pigment-protein complexes Photosystem II (PSII) and Photosystem I (PSI) embedded within the<br/>thylakoid membrane of photoautotrophs. The function of these pigment-protein complexes are<br/>conserved between all photoautotrophs, however, the oligomeric structure, as well as the<br/>spectroscopic properties of the PSI

In oxygenic photosynthesis, conversion of solar energy to chemical energy is catalyzed by the<br/>pigment-protein complexes Photosystem II (PSII) and Photosystem I (PSI) embedded within the<br/>thylakoid membrane of photoautotrophs. The function of these pigment-protein complexes are<br/>conserved between all photoautotrophs, however, the oligomeric structure, as well as the<br/>spectroscopic properties of the PSI complex, differ. In early evolving photoautotrophs, PSI<br/>exists in a trimeric organization, but in later evolving species this was lost and PSI exists solely<br/>as a monomer. While the reasons for a change in oligomerization are not fully understood, one<br/>of the 11 subunits within cyanobacterial PSI, PsaL, is thought to be involved in trimerization<br/>through the coordination of a calcium ion in an adjacent monomer. Recently published<br/>structures have demonstrated that PSI complexes are capable of trimerization without<br/>coordinating the calcium ion within PsaL.<br/>5 Here we explore the role the calcium ion plays in both<br/>the oligomeric and spectroscopic properties in PSI isolated from Synechocystis sp. PCC 6803.

ContributorsVanlandingham, Jackson R (Author) / Mazor, Yuval (Thesis director) / Mills, Jeremy (Committee member) / School of Life Sciences (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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DescriptionThis creative project provides documentation and an exploration of my interactions with individuals encountered while hitchhiking up the west coast.
ContributorsGerber, Evan Howard (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / W. P. Carey School of Business (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Throughout history humans have had to adapt to changing conditions in order to survive. Food shortages are one of the major pressures that have shaped past populations. Because of this, the human body has many physiological adaptations that allow it to go extended periods of time consuming little to

Throughout history humans have had to adapt to changing conditions in order to survive. Food shortages are one of the major pressures that have shaped past populations. Because of this, the human body has many physiological adaptations that allow it to go extended periods of time consuming little to no food. These adaptations also allow the body to recover quickly once food becomes available. They include changes in metabolism that allow different fuel sources to be used for energy, the storing of excess energy absorbed from food in the forms of glycogen and fat to be used in between meals, and a reduction in the basal metabolic rate in response to starvation, as well as physiological changes in the small intestines. Even in places where starvation is not a concern today, these adaptations are still important as they also have an effect on weight gain and dieting in addition to promoting survival when the body is in a starved state.

Disclaimer: The initial goal of this project was to present this information as a podcast episode as a part of a series aimed at teaching the general public about human physiological adaptations. Due to the circumstances with COVID-19 we were unable to meet to make a final recording of the podcast episode. A recording of a practice session recorded earlier in the year has been uploaded instead and is therefore only a rough draft.
ContributorsPhlipot, Stephanie Anne (Author) / Hyatt, JP (Thesis director) / Kingsbury, Jeffrey (Committee member) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
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Description
Coral reefs are diverse marine ecosystems, where reef building corals provide both the structure of the habitat as well as the primary production through their symbiotic algae, and alongside algae living on the reef itself, are the basis of the food web of the reef. In this way, coral reefs

Coral reefs are diverse marine ecosystems, where reef building corals provide both the structure of the habitat as well as the primary production through their symbiotic algae, and alongside algae living on the reef itself, are the basis of the food web of the reef. In this way, coral reefs are the ocean's "forests" and are estimated to support 25% of all marine species. However, due to the large size of a coral reef, the relative inaccessibility and the reliance on in situ surveying methods, our current understanding of reefs is spatially limited. Understanding coral reefs from a more spatially complete perspective will offer insight into the ecological factors that contribute to coral reef vitality. This has become a priority in recent years due to the rapid decline of coral reefs caused by mass bleaching. Despite this urgency, being able to assess the entirety of a coral reef is physically difficult and this obstacle has not yet been overcome. However, similar difficulties have been addressed in terrestrial ecosystems by using remote sensing methods, which apply hyperspectral imaging to assess large areas of primary producers at high spatial resolutions. Adapting this method of remote spectral sensing to assess coral reefs has been suggested, but in order to quantify primary production via hyper spectral imaging, light-use efficiencies (LUEs) of coral reef communities need to be known. LUEs are estimations of the rate of carbon fixation compared to incident absorbed light. Here, I experimentally determine LUEs and report on several parameters related to LUE, namely net productivity, respiration, and light absorbance for the main primary producers in coral reefs surrounding Bermuda, which consist of algae and coral communities. The derived LUE values fall within typical ranges for LUEs of terrestrial ecosystems, with LUE values for coral averaging 0.022 ± 0.002 mol O2 mol photons-1 day-1 at a water flow rate of 17.5 ± 2 cm s^(-1) and 0.049 ± 0.011 mol O2 mol photons-1 day-1 at a flow rate of 32 ± 4 cm s^(-1) LUE values for algae averaged 0.0335 ± 0.0048 mol O2 mol photons-1 day-1 at a flow rate of 17.5 ± 2 cm s^(-1). These values allow insight into coral reef productivity and opens the door for future remote sensing applications.
ContributorsFlesher, David A (Author) / Neuer, Susanne (Thesis director) / Redding, Kevin (Committee member) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The Scientist in Me is an original children’s book, authored by Annmarie Barton and illustrated by Alison Lane, that explores the lives and specialties of five remarkable scientists from historically underrepresented backgrounds: Mary Anning, James Pollack, Temple Grandin, Percy Lavon Julian, and Ayah Bdeir. In the book, each scientist has

The Scientist in Me is an original children’s book, authored by Annmarie Barton and illustrated by Alison Lane, that explores the lives and specialties of five remarkable scientists from historically underrepresented backgrounds: Mary Anning, James Pollack, Temple Grandin, Percy Lavon Julian, and Ayah Bdeir. In the book, each scientist has an “Experiment” section that is meant to encourage children to immerse themselves in activities relating to the scientists’ areas of study. We believe that diversity in science is crucial for advancement, and therefore hope to inspire the next generation of scientists through immersion and representation.
ContributorsLane, Alison (Co-author) / Barton, Annmarie (Co-author) / Klemaszewski, James (Thesis director) / Fette, Donald (Committee member) / School of Molecular Sciences (Contributor) / School of Art (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
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Description
The heliobacteria, a family of anoxygenic phototrophs, are significant to photosynthesis evolution research, as they possess the simplest known photosynthetic apparatus. Although they are photoheterotrophs in the light, the heliobacteria may also grow chemotrophically via pyruvate metabolism in the absence of light. In Heliobacterium modesticaldum, the cytochrome bc complex is

The heliobacteria, a family of anoxygenic phototrophs, are significant to photosynthesis evolution research, as they possess the simplest known photosynthetic apparatus. Although they are photoheterotrophs in the light, the heliobacteria may also grow chemotrophically via pyruvate metabolism in the absence of light. In Heliobacterium modesticaldum, the cytochrome bc complex is responsible for oxidizing menaquinol and reducing cytochrome c553 in the electron flow cycle used for phototrophy. However, there is no known electron acceptor for cytochrome c553 other than the photosynthetic reaction center. Therefore, it was hypothesized that the cytochrome bc complex is necessary for phototrophy, but unnecessary for chemotrophic growth in the dark. Under this hypothesis, a mutant of H. modesticaldum lacking the cytochrome bc complex was predicted to be viable, but non-phototrophic. In this project, a two-step method for CRISPR-based genome editing was used in H. modesticaldum to delete the genes encoding the cytochrome bc complex. Genotypic analysis verified the deletion of the petC, B, D, and A genes encoding the catalytic components of complex. Spectroscopic studies revealed that re-reduction of cytochrome c553 after flash-induced photo-oxidation was ~130 to 190 times slower in the ∆petCBDA mutant compared to wildtype, phenotypically confirming the removal of the cytochrome bc complex. The resulting ∆petCBDA mutant was unable to grow phototrophically, instead relying on pyruvate metabolism to grow chemotrophically as does wildtype in the dark.
ContributorsLeung, Sabrina (Author) / Redding, Kevin (Thesis director) / Liu, Wei (Committee member) / Vermaas, Wim (Committee member) / School of Molecular Sciences (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
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Description
University College Groningen, in the Netherlands, is one of two Barrett Exchange programs. These programs allow for Barrett students to receive honors credit for their classes while studying abroad. While these programs are intriguing, there are no resources on the Barrett website that provide student accounts of the experiences. For

University College Groningen, in the Netherlands, is one of two Barrett Exchange programs. These programs allow for Barrett students to receive honors credit for their classes while studying abroad. While these programs are intriguing, there are no resources on the Barrett website that provide student accounts of the experiences. For the UCG exchange, there was only a link to the UCG website which was not extremely helpful for getting an understanding of what you will truly be getting yourself into while abroad. The solution that was decided upon was to create a vlog website for Barrett students to use as a resource when looking into the program. The site contains both person experiences from students, as well has helpful tips and tricks of how to maneuver your stay in the Netherlands. Overall, there were 8 videos created and 9 posts that can be used as resources for future Barrett students. The ‘Who are We?’, ‘Why a Barrett Exchange?’, ‘First Impressions and Adjusting to the Dutch Lifestyle’, and ‘Welcome Weeks’ posts contain testimony from two other Barrett students and myself who went on the exchange during the Fall, 2018 semester. The ‘Vistmarkt’ and ‘UCG Tour’ posts contain videos that show students places they will be able to venture to in the Netherlands. The ‘Travels Tips’ and ‘UCG Curriculum’ posts contain videos that have numerous tips for students who choose this exchange as their study abroad program they wish to participate in. The final post is called ‘Next Steps’ and it is meant for future students who wish to update and extend the knowledge that is presents on the website so that students can get the most up-to-date information. This website was created to give Barrett students a better understanding of the life-changing experience they are about to embark on.
ContributorsBarda, Taylor (Author) / Scott Lynch, Jacquelyn (Thesis director) / Chiu, Roland (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12
Description
The tarot is a means of communication with the world. It allows readers to interpret signs from their surroundings, gather information, and use this information to make inferences about a posed question. Its origins can be found in mid-15th century Europe as playing cards with four suits commonly used for

The tarot is a means of communication with the world. It allows readers to interpret signs from their surroundings, gather information, and use this information to make inferences about a posed question. Its origins can be found in mid-15th century Europe as playing cards with four suits commonly used for gambling. Several hundred years later during the 18th century, it began to be used as a tool for divination; the Major Arcana, a set of 22 trump cards representing various archetypes, evolved as a supplement to a new tarot that has become associated with mysticism. The tarot’s foundation is based on archetypes that build society. It can serve as a visual lens to understand the experiences, thoughts, and actions of a person posing a question, allowing the reader to offer a solution by understanding and interpreting the specific visual language of a deck.
Hinduism is one of the oldest religions in the world and one of the most practiced today. It is full of fantastical myths and heroic legends, as well as undercurrents of feminism contrasted with misogyny and patriarchy. Hindu myths are contradictory as stories have evolved over time and have been retold with millions of differing perspectives.
In my thesis, I portrayed the 22 archetypes of the Major Arcana of the tarot through the lens of Hindu mythology as well as the broader pan-Indian culture. I include ancient stories and references to modern social issues. I visually communicated the connections between characters of Hindu mythology and the archetypes of the tarot with 22 watercolor paintings. This project was an opportunity to explore both the tarot through Hinduism, vice-versa. It allowed for the development of a deeper connection with spirituality and religion, along with a greater understanding of visual communication.
ContributorsHarve, Prathima (Author) / Jenik, Adriene (Thesis director) / Codell, Julie (Committee member) / Zirbel, Lisa (Committee member) / School of Molecular Sciences (Contributor) / School of Art (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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Description
Within our current educational infrastructure, there’s a lack of substantial preventive care knowledge present among elementary schoolchildren. With education cuts occurring statewide, many schools are left impoverished and schools are incapable of implementing various programs to benefit their local communities. This endeavor aims to visit public and charter elementary schools

Within our current educational infrastructure, there’s a lack of substantial preventive care knowledge present among elementary schoolchildren. With education cuts occurring statewide, many schools are left impoverished and schools are incapable of implementing various programs to benefit their local communities. This endeavor aims to visit public and charter elementary schools in the Phoenix Valley to educate youth regarding easily avoidable health risks by implementing healthy eating habits and exercise. Project BandAid will immerse students ages 7-9 in hands-on activities to enhance their knowledge on hygiene, healthy eating habits, and safety. This project incorporated funding from the Woodside Community Action Grant and Barrett, the Honors College as well as the help from Alpha Epsilon Delta (AED) volunteers.
ContributorsCovarrubias, Sidney Alicia (Co-author) / Kothari, Karishma (Co-author) / John, Benson (Co-author) / Fette, Donald (Thesis director) / Holechek, Susan (Committee member) / Sanford School of Social and Family Dynamics (Contributor) / School of Molecular Sciences (Contributor) / School for the Future of Innovation in Society (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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Description
The Heliobacterial Reaction Center (HbRC) is the simplest Type I Reaction Center (RC) known today. However, upon illumination it has been found to produce menaquinol, and this has led to experiments investigating the function of this reduction scheme. The goal of the experiment was to investigate the mechanisms of menaquinol

The Heliobacterial Reaction Center (HbRC) is the simplest Type I Reaction Center (RC) known today. However, upon illumination it has been found to produce menaquinol, and this has led to experiments investigating the function of this reduction scheme. The goal of the experiment was to investigate the mechanisms of menaquinol production through the use of Photosystem II (PSII) herbicides that are known to inhibit the QB quinone site in Type II RCs. Seven herbicides were chosen, and out of all of them terbuthylazine showed the greatest effect on the RC in isolated membranes when Transient Absorption Spectroscopy was used. In addition, terbuthylazine decreased menaquinone reduction to menaquinol by ~72%, slightly more than the reported effect of teburtryn (68%)1. In addition, terbuthylazine significantly impacted growth of whole cells under high light more than terbutryn.
ContributorsOdeh, Ahmad Osameh (Author) / Redding, Kevin (Thesis director) / Woodbury, Neal (Committee member) / Allen, James (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05