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- All Subjects: intermediary ecosystem metabolism
- All Subjects: Wastewater Treatment Plants
- Creators: Childers, Daniel
Description
Under current climate conditions northern peatlands mostly act as C sinks; however, changes in climate and environmental conditions, can change the soil carbon decomposition cascade, thus altering the sink status. Here I studied one of the most abundant northern peatland types, poor fen, situated along a climate gradient from tundra (Daring Lake, Canada) to boreal forest (Lutose, Canada) to temperate broadleaf and mixed forest (Bog Lake, MN and Chicago Bog, NY) biomes to assess patterns of microbial abundance across the climate gradient. Principal component regression analysis of the microbial community and environmental variables determined that mean annual temperature (MAT) (r2=0.85), mean annual precipitation (MAP) (r2=0.88), and soil temperature (r2=0.77), were the top significant drivers of microbial community composition (p < 0.001). Niche breadth analysis revealed the relative abundance of Intrasporangiaceae, Methanobacteriaceae and Candidatus Methanoflorentaceae fam. nov. to increase when MAT and MAP decrease. The same analysis showed Spirochaetaceae, Methanosaetaceae and Methanoregulaceae to increase in relative abundance when MAP, soil temperature and MAT increased, respectively. These findings indicated that climate variables were the strongest predictors of microbial community composition and that certain taxa, especially methanogenic families demonstrate distinct patterns across the climate gradient. To evaluate microbial production of methanogenic substrates, I carried out High Resolution-DNA-Stable Isotope Probing (HR-DNA-SIP) to evaluate the active portion of the community’s intermediary ecosystem metabolic processes. HR-DNA-SIP revealed several challenges in efficiency of labelling and statistical identification of responders, however families like Veillonellaceae, Magnetospirillaceae, Acidobacteriaceae 1, were found ubiquitously active in glucose amended incubations. Differences in metabolic byproducts from glucose amendments show distinct patterns in acetate and propionate accumulation across sites. Families like Spirochaetaceae and Sphingomonadaceae were only found to be active in select sites of propionate amended incubations. By-product analysis from propionate incubations indicate that the northernmost sites were acetate-accumulating communities.
These results indicate that microbial communities found in poor fen northern peatlands are strongly influenced by climate variables predicted to change under current climate scenarios. I have identified patterns of relative abundance and activity of select microbial taxa, indicating the potential for climate variables to influence the metabolic pathway in which carbon moves through peatland systems.
ContributorsSarno, Analissa Flores (Author) / Cadillo-Quiroz, Hinsby (Thesis advisor) / Garcia-Pichel, Ferran (Committee member) / Krajmalnik-Brown, Rosa (Committee member) / Childers, Daniel (Committee member) / Arizona State University (Publisher)
Created2022
Description
Plastics are an emerging issue in aquatic ecosystems due to their slow degradation and ability to fragment into smaller more mobile parts. Concluding this process, plastics <5mm are categorized as Microplastics, MPs. Currently, the majority of MP studies bring attention to marine pollution and the impacts that follow. However, it remains a high priority to understand how MPs move through urban aquatic environments, and the impacts this may have for surrounding urban ecosystems. Little is known about how MPs move through tertiary treated wastewater plants, such as constructed wetlands, and how much, if any, remain trapped in abiotic and biotic material such as soil or plant life, respectively. An analysis of MP distribution using Tres Rios, a tertiary wastewater treatment wetland, as the study site may help to shed light on the source-occurrences of MPs. Microplastics extraction was performed on soil, plant, and water samples that were collected along major access points within the system with emphasis on inflow and outflow. The inflow of the wetland receives between 246-398 MPs/L vs the outflow of 90-199 MPs/L. Tres Rios soil concentrations ranged between 1,017-10,100 per kg and 133-700 MPs per kg in sampled vegetation throughout the wetland. The distribution of soil and vegetation samples differed throughout Tres Rios, as soil sampled exhibited higher quantities towards inflow site and vegetation MP occurrences were increased throughout the middle of the system. Additionally, this study aimed to determine if seasonality impacted the concentration of plastics seen throughout the system. There was no evidence that suggested seasonal variations were occurring in any sample type. Atmospheric deposition fluxes of microplastics were considered as a potential additional influx but even at the measured 1510 MP m-2 day-1 they were small compared to the water influx. Overall, the results suggest that the Tres Rios wetland removed 55% of the microplastics it receives and hence performs a substantial ecosystem service.
ContributorsCisco, Jordan (Author) / Green, Douglas (Thesis advisor) / Herckes, Pierre (Thesis advisor) / Childers, Daniel (Committee member) / Arizona State University (Publisher)
Created2023