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Investigating the Phenotypical Effects of An Adaptable Multi-Modality pH-Sensitive Nanoprobe for Real-Time Monitoring of Engineered Cells and Tissues.

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Cells and tissues play an important role in disease modelling, drug screening and in regenerative medicine applications. In the development and subsequent usage of cells and tissues, it is imperative that the viability of the construct is always assessed. According to BioFab USA and the National Cell Manufacturing Consortium, in-line…

Cells and tissues play an important role in disease modelling, drug screening and in regenerative medicine applications. In the development and subsequent usage of cells and tissues, it is imperative that the viability of the construct is always assessed. According to BioFab USA and the National Cell Manufacturing Consortium, in-line monitoring of manufactured tissues is critical for downstream applications in disease modelling, drug screening and regenerative medicine. The intracellular pH of cells characterizes different activities of the cell including metabolism, proliferation, differentiation, and apoptosis and provide a great opportunity for the assessment of cell and tissue viability.Current technologies have utilized fluorescence probes, MRI, PET and CT techniques to measure cellular pH and to determine cellular and tissue viability. The current technologies have been limited by several factors including the use of custom hardware and software, incompatibility with different culture systems, tissues, and cell types, and the inability to scale into the existing cell and tissue production process. Ratiometric probes for measuring and analyzing of pH has generated scientific interest recently as it provides an accurate measurement of pH-independent of probe concentration or excitation energy. Previously, a team of scientist from Arizona State University collaborating with others developed an adaptable multimodality pH-sensitive probe for non-invasive real-time monitoring of cells and tissues to solve the current problem. In this study, the phenotypical effects of the pH probe were investigated using proliferative and non-proliferative cells in different culture systems. Specifically, the efficiency of the probes in labelling cells in 2D, microcarriers and Matrigel culture systems was determined. The proliferation of cells and cell phenotypes were not affected by labelling with the pH probe and pH dependency were demonstrated in all culture systems.
ContributorsEssuman, Albert (Author) / Brafman, David DB (Thesis advisor) / Weaver, Jessica JW (Committee member) / Kodibagkar, Vikram VK (Committee member) / Arizona State University (Publisher)
Created2021