Full metadata
Title
Highly Multiplexed Single Cell in situ Protein Analysis with Cleavable Fluorescent Probes
Description
Measurements of different molecular species from single cells have the potential to reveal cell-to-cell variations, which are precluded by population-based measurements. An increasing percentage of researches have been focused on proteins, for its central roles in biological processes. Immunofluorescence (IF) has been a well-established protein analysis platform. To gain comprehensive insights into cell biology and diagnostic pathology, a crucial direction would be to increase the multiplexity of current single cell protein analysis technologies.
An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of highly multiplexed single cell in situ protein profiling. These highly multiplexed imaging-based platforms have the potential to quantify more than 100 protein targets in cultured cells and more than 50 protein targets in single cells in tissues.
This approach has been successfully applied in formalin-fixed paraffin-embedded (FFPE) brain tissues. Multiplexed protein expression level results reveal neuronal heterogeneity in the human hippocampus.
An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of highly multiplexed single cell in situ protein profiling. These highly multiplexed imaging-based platforms have the potential to quantify more than 100 protein targets in cultured cells and more than 50 protein targets in single cells in tissues.
This approach has been successfully applied in formalin-fixed paraffin-embedded (FFPE) brain tissues. Multiplexed protein expression level results reveal neuronal heterogeneity in the human hippocampus.
Date Created
2019
Contributors
- Liao, Renjie (Author)
- Guo, Jia (Thesis advisor)
- Borges, Chad (Committee member)
- Liu, Yan (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
ix, 125 pages : illustrations (some color)
Language
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.53605
Statement of Responsibility
by Renjie Liao
Description Source
Viewed on October 9, 2019
Level of coding
full
Note
Partial requirement for: Ph.D., Arizona State University, 2019
Note type
thesis
Includes bibliographical references (pages 105-120)
Note type
bibliography
Field of study: Chemistry
System Created
- 2019-05-15 12:27:43
System Modified
- 2021-08-26 09:47:01
- 2 years 7 months ago
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