Description

Recombinases are powerful tools for genome engineering and synthetic biology, however recombinases are limited by a lack of user-programmability and often require complex directed-evolution experiments to retarget specificity. Conversely, CRISPR

Recombinases are powerful tools for genome engineering and synthetic biology, however recombinases are limited by a lack of user-programmability and often require complex directed-evolution experiments to retarget specificity. Conversely, CRISPR systems have extreme versatility yet can induce off-target mutations and karyotypic destabilization. To address these constraints we developed an RNA-guided recombinase protein by fusing a hyperactive mutant resolvase from transposon TN3 to catalytically inactive Cas9.

Reuse Permissions
  • 2.06 MB application/pdf

    Download count: 0

    Details

    Contributors
    Date Created
    • 2018
    Resource Type
  • Text
  • Collections this item is in
    Note
    • Partial requirement for: M.S., Arizona State University, 2018
      Note type
      thesis
    • Includes bibliographical references (pages 16-18)
      Note type
      bibliography
    • Field of study: Biology

    Citation and reuse

    Statement of Responsibility

    by Kylie S Standage-Beier

    Machine-readable links