Description

A novel clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) tool for simultaneous gene editing and regulation was designed and tested. This study used the CRISPR-associated protein 9 (Cas9) endonuclease in

A novel clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) tool for simultaneous gene editing and regulation was designed and tested. This study used the CRISPR-associated protein 9 (Cas9) endonuclease in complex with a 14-nucleotide (nt) guide RNA (gRNA) to repress a gene of interest using the Krüppel associated box (KRAB) domain, while also performing a separate gene modification using a 20-nt gRNA targeted to a reporter vector.

Reuse Permissions
  • 2.31 MB application/pdf

    Download count: 0

    Details

    Contributors
    Date Created
    • 2018
    Resource Type
  • Text
  • Collections this item is in
    Note
    • Vita
    • Partial requirement for: M.S., Arizona State University, 2018
      Note type
      thesis
    • Includes bibliographical references (pages 60-63)
      Note type
      bibliography
    • Field of study: Molecular and cellular biology

    Citation and reuse

    Statement of Responsibility

    by Jennifer E. Chapman

    Machine-readable links