Full metadata
Title
Insights towards developing regenerative therapies: the lizard, Anolis carolinensis, as a genetic model for regeneration in amniotes
Description
Damage to the central nervous system due to spinal cord or traumatic brain injury, as well as degenerative musculoskeletal disorders such as arthritis, drastically impact the quality of life. Regeneration of complex structures is quite limited in mammals, though other vertebrates possess this ability. Lizards are the most closely related organism to humans that can regenerate de novo skeletal muscle, hyaline cartilage, spinal cord, vasculature, and skin. Progress in studying the cellular and molecular mechanisms of lizard regeneration has previously been limited by a lack of genomic resources. Building on the release of the genome of the green anole, Anolis carolinensis, we developed a second generation, robust RNA-Seq-based genome annotation, and performed the first transcriptomic analysis of tail regeneration in this species. In order to investigate gene expression in regenerating tissue, we performed whole transcriptome and microRNA transcriptome analysis of regenerating tail tip and base and associated tissues, identifying key genetic targets in the regenerative process. These studies have identified components of a genetic program for regeneration in the lizard that includes both developmental and adult repair mechanisms shared with mammals, indicating value in the translation of these findings to future regenerative therapies.
Date Created
2015
Contributors
- Hutchins, Elizabeth (Author)
- Kusumi, Kenro (Thesis advisor)
- Rawls, Jeffrey A. (Committee member)
- Denardo, Dale F. (Committee member)
- Huentelman, Matthew J. (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
vii, 119 pages : color illustrations
Language
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.29953
Statement of Responsibility
by Elizabeth Hutchins
Description Source
Retrieved on Aug. 14, 2015
Level of coding
full
Note
Partial requirement for: Ph.D., Arizona State University, 2015
Note type
thesis
Includes bibliographical references (pages 90-119)
Note type
bibliography
Field of study: Molecular and cellular biology
System Created
- 2015-06-01 08:14:13
System Modified
- 2021-08-30 01:28:40
- 2 years 7 months ago
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