Full metadata
Title
An in vitro selected sequence capable of ultrahigh transgene expression in vaccinia virus infected cells
Description
Recombinant protein expression is essential to biotechnology and molecular medicine, but facile methods for obtaining significant quantities of folded and functional protein in mammalian cell culture have been lacking. Here I describe a novel 37-nucleotide in vitro selected sequence that promotes unusually high transgene expression in a vaccinia driven cytoplasmic expression system. Vectors carrying this sequence in a monocistronic reporter plasmid produce >1,000-fold more protein than equivalent vectors with conventional vaccinia promoters. Initial mechanistic studies indicate that high protein expression results from dual activity that impacts both transcription and translation. I suggest that this motif represents a powerful new tool in vaccinia-based protein expression and vaccine development technology.
Date Created
2012
Contributors
- Flores, Julia Anne (Author)
- Chaput, John C (Thesis advisor)
- Jacobs, Bertram (Committee member)
- LaBaer, Joshua (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
vi, 56 p. : ill. (some col.)
Language
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.14728
Statement of Responsibility
by Julia Anne Flores
Description Source
Retrieved on April 3, 2013
Level of coding
full
Note
Partial requirement for: M.S., Arizona State University, 2012
Note type
thesis
Includes bibliographical references (p. 49-56)
Note type
bibliography
Field of study: Biochemistry
System Created
- 2012-08-24 06:20:55
System Modified
- 2021-08-30 01:47:40
- 2 years 8 months ago
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