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Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on dee

Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on deep transcriptome sequences of adult skeletal muscle, lung, brain, and blood. The draft genome assembly for G. agassizii has a scaffold N50 length of 252 kbp and a total length of 2.4 Gbp. Genome annotation reveals 20,172 protein-coding genes in the G. agassizii assembly, and that gene structure is more similar to chicken than other turtles. We provide a series of comparative analyses demonstrating (1) that turtles are among the slowest-evolving genome-enabled reptiles, (2) amino acid changes in genes controlling desert tortoise traits such as shell development, longevity and osmoregulation, and (3) fixed variants across the Gopherus species complex in genes related to desert adaptations, including circadian rhythm and innate immune response. This G. agassizii genome reference and annotation is the first such resource for any tortoise, and will serve as a foundation for future analysis of the genetic basis of adaptations to the desert environment, allow for investigation into genomic factors affecting tortoise health, disease and longevity, and serve as a valuable resource for additional studies in this species complex.

Data Availability: All genomic and transcriptomic sequence files are available from the NIH-NCBI BioProject database (accession numbers PRJNA352725, PRJNA352726, and PRJNA281763). All genome assembly, transcriptome assembly, predicted protein, transcript, genome annotation, repeatmasker, phylogenetic trees, .vcf and GO enrichment files are available on Harvard Dataverse (doi:10.7910/DVN/EH2S9K).

ContributorsTollis, Marc (Author) / DeNardo, Dale F (Author) / Cornelius, John A (Author) / Dolby, Greer A (Author) / Edwards, Taylor (Author) / Henen, Brian T. (Author) / Karl, Alice E. (Author) / Murphy, Robert W. (Author) / Kusumi, Kenro (Author)
Created2017-05-31
Description
A presentation describing technical work, community reaction and publicity associated with an online collection of Arizona Japanese-American internment camp newsletters and related archival materials developed in 2017. The presentation was given at the Arizona Library Association conference in October 2017 and this revised version was presented at the Arizona Archives

A presentation describing technical work, community reaction and publicity associated with an online collection of Arizona Japanese-American internment camp newsletters and related archival materials developed in 2017. The presentation was given at the Arizona Library Association conference in October 2017 and this revised version was presented at the Arizona Archives Summit on February 1, 2018.
ContributorsSpindler, Rob (Author)
Created2018-01-18
Description

Materials produced for the workshop hosted by the Humane Cities Initiative, Institute for Humanities Research, Arizona State University. Participants were sent this package of archival materials and links to brief online sources for advance readings. Also enclosed are PDF renderings of introductory powerpoint files by Spindler and Pagan, and an

Materials produced for the workshop hosted by the Humane Cities Initiative, Institute for Humanities Research, Arizona State University. Participants were sent this package of archival materials and links to brief online sources for advance readings. Also enclosed are PDF renderings of introductory powerpoint files by Spindler and Pagan, and an audio recording of the discussion joined in progress.

ContributorsSpindler, Rob (Compiler, Author) / Pagán, Eduardo Obregón, 1960- (Author)
Created2018-03-13
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Description

Presentation slides regarding the history of Victory Village, the trailer park built in 1945-46 to provide housing for WWII Veterans and their families at Arizona State University's Tempe campus. A presentation of research from University Archives records conducted in the summer of 2018. The presentation was videotaped as a lecture

Presentation slides regarding the history of Victory Village, the trailer park built in 1945-46 to provide housing for WWII Veterans and their families at Arizona State University's Tempe campus. A presentation of research from University Archives records conducted in the summer of 2018. The presentation was videotaped as a lecture for Professor Volker Benkert's online World War II history class.

ContributorsSpindler, Rob (Author)
Created2019-09-30
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Description

Powerpoint slides from Spindler's presentation at the 56th annual Arizona History Convention in Tucson, Arizona, April 24th, 2015. Details of the 1993-1995 U.S. District Court orders directing the corporate archives to Arizona State University and ASU's efforts to recover information from an obsolete digital imaging system are presented.

ContributorsSpindler, Rob (Author)
Created2015-04-24
DescriptionPowerpoint slides, audio clips and transcriptions from the presentation describing the work of Lincoln and Eleanor Ragsdale and civil rights actions at Phoenix from 1963-1964.
ContributorsSpindler, Rob (Author)
Created2017-02-18
Description

This research study will discover and evaluate information about existing crowdsourcing or participatory archives projects devoted to archival description, indexing or transcription. Many related projects that use crowdsourcing for collecting archival materials from the public are not specifically addressed here. In this research, the author has specifically sought evaluative information

This research study will discover and evaluate information about existing crowdsourcing or participatory archives projects devoted to archival description, indexing or transcription. Many related projects that use crowdsourcing for collecting archival materials from the public are not specifically addressed here. In this research, the author has specifically sought evaluative information about exemplary projects that can lead to useful specifications for a participatory archives system at Arizona State University Libraries.

ContributorsSpindler, Rob (Author)
Created2014-06-30
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Description

Presentation slides describing an Arizona Centennial project to build a digital library and facilitate community discussion about why people came to Arizona, why they stayed, and why they left. Presentation was made at Friendship Village, Tempe AZ, November 2011.

ContributorsSpindler, Rob (Author)
Created2011-10-31
Description

The Task Force of thirty faculty members and academic professionals was charged by the University Libraries and the Graduate College to "examine the potential for implementing a university program for Electronic Theses and Dissertations (ETDs). The Task Force was specifically asked to collect information about existing programs elsewhere, identify ASU

The Task Force of thirty faculty members and academic professionals was charged by the University Libraries and the Graduate College to "examine the potential for implementing a university program for Electronic Theses and Dissertations (ETDs). The Task Force was specifically asked to collect information about existing programs elsewhere, identify ASU graduate programs that could host pilot programs and prepare a comprehensive white paper..." with a recommended plan for implementation.

ContributorsSpindler, Rob (Compiler) / Losse, Deborah (Compiler)
Created2001-05-01