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ABSTRACT Telomeres are vital in protecting chromosome ends to prevent telomere shortening. Telomerase is a ribonucleoprotein responsible for adding telomere repeats and maintaining telomere length. Telomerase holoenzyme consists of 2 major subcomponents: telomerase reverse transcriptase (TERT) and telomerase RNA (TR). The catalytic subunit is TERT and the subunit that adds deoxyribonucleotide to the ends of chromosome is TR. TR contains an alignment portion and a template portion. Japanese Medaka (Oryzias latipes) has 4 nucleotide bases in its alignment region, which is similar to the 5-nucleotide bases in the human telomerase RNA alignment region. Because of the similar alignment region length, Japanese Medaka with 24 chromosomes was chosen to be used in this study. The question in this research was whether we could overcome heterogeneity. It was expected that when breeding short mean telomere length fish with another short mean telomere length fish, the new generation would have homogeneity. If short average telomere length fish and long average telomere length fish were to breed, the next generation fish would have heterogeneity in their average telomere length. In order to make a strong result statement further research needs to be done. The results from this study have somewhat supported the hypothesis, but will need additional information for a stronger validation. There were two inbreedings of short mean telomere length fish with another short telomere length; however, only one of the inbreeding pairs produced a fish with homogeneity (and supported the hypothesis). The other inbreeding pair depicted a large smear, a sign of heterogeneity. This may be due to a mutation in the subtelomeric portion. The method used to measure average telomere length was the terminal restriction fragment assay. Future research will involve using a different technique, quantitative fluorescence in sifu hybridizatrort to measure a more accurate telomere length of each chromosome.
ContributorsYee, Stephanie (Author) / Chen, Julian (Thesis director) / Stout, Valerie (Committee member) / Qi, Xiaodong (Committee member) / Barrett, The Honors College (Contributor)
Created2012-05