The heat treatment of lithic raw material is a globally dispersed technology that improves the flaking quality of toolstone. While not all types of stone respond to heat treatment, many forms of microcrystalline silicates do, including jasper. Here, we aim to better understand how Perkinsville jasper responds to heat treatment. Perkinsville jasper occurs in the Perkinsville Valley of Yavapai County, Arizona, and was utilized prehistorically by the Prescott, Sinagua, and Hohokam cultures. For our study, we collected seven boulders of jasper from private land (with permission) in Yavapai County. These boulders were flintknapped into 74 spalls which were subsequently heated in an electric kiln using 20 treatment protocols with systematically varying combinations of maximum temperature and maximum heating times. Afterward, we compared multiple quantitative and qualitative characteristics of unheated and heated flakes taken from the same nodule pre- and post-heat treatment. Our heating protocol allows us to determine an ‘optimal heating context’ for Perkinsville jasper and to better understand how variation in time and temperature influences flaking quality of the stone. Lastly, this research develops an experimental reference dataset that can be used by other researchers studying raw material use and heat treatment in the Southwest United States.

With the passage of the Affordable Care Act, the health system in the United States is now being further challenged. There is bipartisan debate on how it can be reconstructed: one party states that the government plays too big of a role, while the other believes it plays too little. Regardless, Americans want change. Reconstruction is not a new topic by any means, and other countries have been forced to do so due to political violence. This paper explores the history and current healthcare organizations of Japan, Iraq, and Afghanistan. These countries have all encountered major political turmoil, which has led to the rebuilding of their respective healthcare systems. Though the United States is not facing political violence that will necessitate reorganization, the examination of nations that have been forced to do so offers lessons applicable to the healthcare system in the US.

Bermuda Land Snails make up a genus called Poecilozonites that is endemic to Bermuda and is extensively present in its fossil record. These snails were also integral to the creation of the theory of punctuated equilibrium. The DNA of mollusks is difficult to sequence because of a class of proteins called mucopolysaccharides that are present in high concentrations in mollusk tissue, and are not removed with standard DNA extraction methods. They inhibit Polymerase Chain Reactions (PCRs) and interfere with Next Generation Sequencing methods. This paper will discuss the DNA extraction methods that were designed to remove the inhibitory proteins that were tested on another gastropod species (Pomacea canaliculata). These were chosen because they are invasive and while they are not pulmonates, they are similar enough to Bermuda Land Snails to reliably test extraction methods. The methods that were tested included two commercially available kits: the Qiagen Blood and Tissue Kit and the Omega Biotek Mollusc Extraction Kit, and one Hexadecyltrimethylammonium Bromide (CTAB) Extraction method that was modified for use on mollusk tissue. The Blood and Tissue kit produced some DNA, the mollusk kit produced almost none, and the CTAB Extraction Method produced the highest concentrations on average, and may prove to be the most viable option for future extractions. PCRs attempted with the extracted DNA have all failed, though it is likely due to an issue with reagents. Further spectrographic analysis of the DNA from the test extractions has shown that they were successful at removing mucopolysaccharides. When the protocol is optimized, it will be used to extract DNA from the tissue from six individuals from each of the two extant species of Bermuda Land Snails. This DNA will be used in several experiments involving Next Generation Sequencing, with the goal of assembling a variety of genome data. These data will then be used to a construct reference genome for Bermuda Land Snails. The genomes generated by this project will be used in population genetic analyses between individuals of the same species, and between individuals of different species. These analyses will then be used to aid in conservation efforts for the species.

Media witnessing and storytelling for environmental justice (EJ) provide an avenue to understand the relationships between “multiple realities of environmental injury” and to analyze “fleeting phenomena with lasting form; thereby transforming phenomena that are experienced in a plurality of lives into publicly recognized history” (Houston, 2012, 419, 422). This creates opportunities to challenge and eradicate the oppressive structures that deem certain individuals and groups disposable and ultimately protect the possessive investment in whiteness. Therefore, for the purposes of EJ, media witnessing creates space for dynamic, citizen-based storytelling which can undermine narratives that promote the life versus economy framework that has perpetuated oppression, injustice, and state sanctioned violence. Media witnessing in an EJ context demonstrates the potential for collective understanding and action, political opportunities, and healing.<br/>This paper is an analysis of the process of media witnessing in regards to the Flint Water Crisis and the construction of the Dakota Access Pipeline (DAPL) and will apply an EJ lens to this phenomenon. It will discuss how media witnessing in response to these two crises can be used as a precedent for understanding and utilizing this framework and digital storytelling to address the crises of 2020, primarily the COVID-19 pandemic and racial injustice. It will then examine how the intersectionality of race, gender, and age has implications for future media witnessing and storytelling in the context of EJ movements. Finally, it will explain how media witnessing can motivate holistic policymaking in the favor of EJ initiatives and the health and wellbeing of all Americans, as well as how such policymaking and initiatives must acknowledge the double-edged sword that is social media.

The COVID-19 pandemic places significant strain on the U.S. healthcare system due to the high number of coronavirus cases. During the pandemic, there was much unknown about the virus, its course of the disease, COVID-19 diagnosis, treatments, or other imperative information needed to contain the virus. Resources within the healthcare system, such as PPE and healthcare workers, were in short supply and exacerbated the difficulty of managing the viral outbreak. Peer-reviewed articles suggest that telehealth, the application of electronic information and telecommunication technologies in healthcare, proved useful in public health and clinical care during the 2020 public health emergency due to a novel virus. The scoping review broadly assessed themes of telehealth’s strengths and weaknesses during the COVID-19 pandemic. These findings could suggest how virtual medicine may be a helpful tool to improve access in addition to the quality of care in the future of medicine. Assessments of case studies suggest that telehealth helped provide care to large patient volumes by aiding with communication, data collection, triage, remote patient monitoring, and critical care. Limitations of expanding telehealth subsequent to the pandemic include, but not limited to, a lack of national standards for practice and restrictions of utility for certain populations. Populations may include those with low socioeconomic status, specific cultural practices, and beliefs, or physical and cognitive ability barriers. Outlining the benefits and limitations of telehealth may suggest how virtual medicine can provide valuable in day-to-day medical practices and other pathogenic outbreaks.

In 2014 alone, 40% of all drug abuse-related emergency department visits involved cocaine, and despite the detrimental effects there is still no FDA approved treatment for cocaine use disorders (CUDs; Dawn, 2014). Studies show that serotonin 1B receptor (5HT1BR) agonists modulate cocaine abuse-related behaviors in opposite directions depending on the phase of the addiction cycle in male rats. In particular, the selective 5HT1BR agonist, CP94,253, facilitates cocaine intake during maintenance of daily cocaine self-administration. Paradoxically, after 21 days of abstinence, CP94,253 attenuates cocaine intake in male rats on a low effort fixed ratio 5 (FR5) and a high effort progressive ratio (PR) schedule of reinforcement. PR measures motivation as it requires an exponentially increasing number of lever responses to obtain the next reinforcer after a successful reinforcer. In contrast to male rats, we recently found CP94,253 attenuates cocaine intake before and after abstinence on an FR5 schedule of reinforcement in female rats, suggesting the attenuating effects of CP94,253 on cocaine intake is not dependent on a period of abstinence in females. However, the effect of CP94,253 on motivation for cocaine has not yet been examined in female rats. Therefore, we addressed this gap in the present study. Female Sprague-Dawley rats were trained to self-administer 0.375 mg/kg, IV cocaine or to obtain sucrose pellets (45 mg) on a PR schedule of reinforcement and were then pretreated with vehicle or CP94,253 (3.2, 5.6 and 10 mg/kg, SC) prior to their self-administration session. A separate cohort was pretreated with CP94,253 to examine the effects of CP94,253 on cocaine-seeking behavior (i.e., operant responses when cocaine is no longer available) and spontaneous locomotion after 21 or 60 days of abstinence. The preliminary findings show that CP94,253 has minimal impacts on decreasing cocaine intake on a PR schedule in female rats but decreases cue reactivity up to 60 days after abstinence in female rats. These findings suggest that 5-HT1BR agonists may be useful treatments for cocaine craving.

Young adults with type one diabetes mellitus (T1DM) face unique challenges in managing their chronic disease. While simultaneously navigating major life transitions and becoming fully responsible for their diabetes-self management behaviors (DSMB), social support can be an integral part of disease management. Many young adults enroll in college where student organizations are prevalent including diabetes related social groups on some campuses, which provide a rich source of social support for students with diabetes as they transition to greater independence in diabetes management. This study used descriptive analysis and personal network analysis (PNA) to investigate which aspects of being a part of a diabetes related social group and personal networks, in general, are pertinent to successful diabetes management, measured by a Diabetes Self-Management Questionnaire (DSMQ) among 52 young adults with T1DM. The majority of respondents indicated that since being a part of College Diabetes Network (CDN) or another diabetes-related social group, they increased time spent paying attention to, and felt more empowered to make changes to their diabetes management routine, and they were able to generally take better care of their diabetes. Half of respondents noticed their health improved since joining, and over half felt less burdened by their diabetes. Though no personal network measures were highly correlated with higher Diabetes Self-Management Scores, the degree to which health matters were discussed within their personal network was the most associated personal network measure. Our findings help contextualize the ways in which young adults’ DSMB are influenced by participation in diabetes- related social groups, as well as introduce the use of personal network analysis in gauging important aspects of social capital and support in young adults with chronic disease.

This thesis project utilizes Bolman & Deal’s (2017) four frames to analyze how the Internship experience at TigerMountain Foundation, a South Phoenix community garden nonprofit, can be optimized to help the organization more effectively reach its goals. A brief explanation of the organizational context and structure is given as well as an overview of the relationship between community gardening and decreasing recidivism, as well as TigerMountain’s position in a food desert. TigerMountain Foundation can ultimately be framed internally as a human resource and symbolic organization and externally as a political organization. The Internship program presents a political benefit to the organization and can benefit from some human resource and structural additions to the onboarding process and overall experience. The recommended additions include providing a thorough onboarding packet to Interns at orientation that includes a questionnaire, includes a brief overview of the organization in human resource framing, a contact sheet, and instruction sheets for commonly used systems. Other additions to the Internship experience include setting up a ratio of how many Internship hours can be earned at the gardens and farmers’ markets compared to working administratively, requesting that Interns send in their updated availability weekly for scheduling purposes, and the implementation of an “on-call” system for farmers’ market shifts.

Trauma is increasingly experienced by people in transit as border militarization increases migrants’ exposure to violence and forces them into more precarious situations. For queer migrants, this includes situations where they are more likely to experience persecution and sexual violence. This paper explores the availability of care for queer undocumented migrants in the United States after surviving a precarious and potentially deadly journey from their country of origin to the US, as well as forms of alternative care developed by the undocuqueer community. In particular, it focuses on access to care for LGBT migrants, who face stigmatization on multiple levels and as a result are more likely than their straight counterparts to experience extreme mental health consequences pre-, in-, and post-transit. Faced with a number of obstacles that prevent them from receiving appropriate mental health care, the undocuqueer community utilizes various strategies to ensure that the health and needs of the community are supported. I argue that in spite of facing traumatic experiences and being unable to fully access healthcare to alleviate these problems in the US, LGBT migrants demonstrate extreme resilience and resist the mechanisms that otherwise threaten their mental well-being.

This Thesis presentation and book review is on social media manipulation and the issue of media algorithms developing a close minded perspective in individuals. It discusses the mechanics of these algorithms, the definition of social media manipulation, and the neutral negative impacts on the polarization of our country. It also goes into detail on how I applied this research to design projects throughout 4th year of the Visual Communication Design Program.