Filtering by
- Status: Published
Manufacturing anomalies, inaccurate forecasts, and other problems can lead to SC disruptions. Traditional monitoring methods are not sufficient in this respect, because com- plex SCs feature changes in manufacturing tasks (dynamic complexity) and carry a large number of stock keeping units (detail complexity). Problems are easily confounded with normal system variations.
Motivated by these real challenges faced by modern SC, new surveillance solutions are proposed to detect system deviations that could lead to disruptions in a complex SC. To address supply-side deviations, the fitness of different statistics that can be extracted from the enterprise resource planning system is evaluated. A monitoring strategy is first proposed for SCs featuring high levels of dynamic complexity. This presents an opportunity for monitoring methods to be applied in a new, rich domain of SC management. Then a monitoring strategy, called Heat Map Contrasts (HMC), which converts monitoring into a series of classification problems, is used to monitor SCs with both high levels of dynamic and detail complexities. Data from a semiconductor SC simulator are used to compare the methods with other alternatives under various failure cases, and the results illustrate the viability of our methods.
To address demand-side deviations, a new method of quantifying forecast uncer- tainties using the progression of forecast updates is presented. It is illustrated that a rich amount of information is available in rolling horizon forecasts. Two proactive indicators of future forecast errors are extracted from the forecast stream. This quantitative method re- quires no knowledge of the forecasting model itself and has shown promising results when applied to two datasets consisting of real forecast updates.
Overall, this work not only provides insights into the structure-property relationship of 2D pentagonal materials and opens up a new route of studying 2D materials by combining geometry and computational materials science, but also shows the potential applications of 2D pentagonal materials in electronic and magnetic devices.
Rationale: Cell-free protein microarrays display naturally-folded proteins based on just-in-time in situ synthesis, and have made important contributions to basic and translational research. However, the risk of spot-to-spot cross-talk from protein diffusion during expression has limited the feature density of these arrays.
Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot.
Results: Even when proteins of different sizes were displayed within the same feature, they were readily detected using protein-specific antibodies. Protein-protein interactions and serological antibody assays using human viral proteome microarrays demonstrated that comparable hits were detected by M-NAPPA and non-multiplexed NAPPA arrays. An ultra-high density proteome microarray displaying > 16k proteins on a single microscope slide was produced by combining M-NAPPA with a photolithography-based silicon nano-well platform. Finally, four new tuberculosis-related antigens in guinea pigs vaccinated with Bacillus Calmette-Guerin (BCG) were identified with M-NAPPA and validated with ELISA.
Conclusion: All data demonstrate that multiplexing features on a protein microarray offer a cost-effective fabrication approach and have the potential to facilitate high throughput translational research.