Matching Items (15)
152470-Thumbnail Image.png
Description
DNA is a unique, highly programmable and addressable biomolecule. Due to its reliable and predictable base recognition behavior, uniform structural properties, and extraordinary stability, DNA molecules are desirable substrates for biological computation and nanotechnology. The field of DNA computation has gained considerable attention due to the possibility of exploiting the

DNA is a unique, highly programmable and addressable biomolecule. Due to its reliable and predictable base recognition behavior, uniform structural properties, and extraordinary stability, DNA molecules are desirable substrates for biological computation and nanotechnology. The field of DNA computation has gained considerable attention due to the possibility of exploiting the massive parallelism that is inherent in natural systems to solve computational problems. This dissertation focuses on building novel types of computational DNA systems based on both DNA reaction networks and DNA nanotechnology. A series of related research projects are presented here. First, a novel, three-input majority logic gate based on DNA strand displacement reactions was constructed. Here, the three inputs in the majority gate have equal priority, and the output will be true if any two of the inputs are true. We subsequently designed and realized a complex, 5-input majority logic gate. By controlling two of the five inputs, the complex gate is capable of realizing every combination of OR and AND gates of the other 3 inputs. Next, we constructed a half adder, which is a basic arithmetic unit, from DNA strand operated XOR and AND gates. The aim of these two projects was to develop novel types of DNA logic gates to enrich the DNA computation toolbox, and to examine plausible ways to implement large scale DNA logic circuits. The third project utilized a two dimensional DNA origami frame shaped structure with a hollow interior where DNA hybridization seeds were selectively positioned to control the assembly of small DNA tile building blocks. The small DNA tiles were directed to fill the hollow interior of the DNA origami frame, guided through sticky end interactions at prescribed positions. This research shed light on the fundamental behavior of DNA based self-assembling systems, and provided the information necessary to build programmed nanodisplays based on the self-assembly of DNA.
ContributorsLi, Wei (Author) / Yan, Hao (Thesis advisor) / Liu, Yan (Thesis advisor) / Chen, Julian (Committee member) / Gould, Ian (Committee member) / Arizona State University (Publisher)
Created2014
153075-Thumbnail Image.png
Description
Because of its ability to harbor social values, norms, and beliefs, heritage has always been utilized as an ideological vehicle. One prominent example of politicizing heritage is Chinese red tourism, comprised of state-promoted tours to revolutionary memorial sites. It is expected to generate political, economic, and social benefits, particularly to

Because of its ability to harbor social values, norms, and beliefs, heritage has always been utilized as an ideological vehicle. One prominent example of politicizing heritage is Chinese red tourism, comprised of state-promoted tours to revolutionary memorial sites. It is expected to generate political, economic, and social benefits, particularly to reinforce the legitimate leadership of the Chinese Communist Party. Statistics show that dramatic market growth in red tourism has occurred over the past decade. Yet it is still heavily driven by the government and thus whether long-term sustainability can be achieved is still questionable.

This dissertation explores the dynamics of red tourism from the perspective of a meaning-making process, where tourism discourses circulate among the processes of production, transmission, and consumption. The results reveal that higher-level government primarily assumes the leading role, whereas local government is largely excluded from strategy making processes and primarily responsible for implementation and operation. Some dissonance exists between higher and lower-level governments in their goals and involvement in red tourism development. Second, intermediaries are not altruistic and attempt to maximize their own benefits. While site interpreters may provide officially authorized narratives, their primary focus is hosting higher-up administrative visitors. On the contrary, tour guides are more customer-oriented, which may lead to officially undesirable interpretations. Third, driven by multiple motives, tourists have increasingly diverse attitudes towards red heritage and participate in various political and non-political activities. A considerable degree of congruence was found between tourists' participation, motivation, memories, and perception. Quantitative results indicate that the majority of tourists have learned about the political significance and/or content of red heritage, and developed more positive attitudes towards, and support for, the CCP and the government, to a certain extent.

This dissertation contributes to current research by adopting a systematic and emic perspective to explore the dynamics of red tourism. Several conceptual frameworks were developed inductively to describe the meaning-making process. Mixed methods were used to learn about tourists' consumption and perceptions of red heritage. Implications regarding enhancing the effectiveness of the meaning-making process, limitations of the study, and potential directions for future research are also discussed.
ContributorsZhao, Shengnan (Author) / Timothy, Dallen J. (Thesis advisor) / Chhabra, Deepak (Committee member) / Lee, Woojin (Committee member) / Nyaupane, Gyan (Committee member) / Li, Wei (Committee member) / Arizona State University (Publisher)
Created2014
150183-Thumbnail Image.png
Description
The objective of this study was to understand domestic and foreign-born housekeeper's individual perceptions of labor mobility and job satisfaction related to their jobs within the hospitality industry. Literature regarding the bridging of tourism, immigration, and labor supply was addressed to expose broad conceptual frameworks that lead to the development

The objective of this study was to understand domestic and foreign-born housekeeper's individual perceptions of labor mobility and job satisfaction related to their jobs within the hospitality industry. Literature regarding the bridging of tourism, immigration, and labor supply was addressed to expose broad conceptual frameworks that lead to the development of this study. More specifically, literature regarding labor mobility within tourism industries, migrant decision making, and barriers to mobility and immigration helped to construct a narrowed conceptual framework specific to hospitality labor in Phoenix, Arizona. Similar and previous studies focused on perceived labor mobility during significant economic or industry shifts. This study included the addition of a policy factor to help determine to what degree state policy change effected hospitality workers' perceived labor mobility. Arizona's recently passed and implemented legislative act SB1070 regards immigrant identification and employment, and enforcement of the act in the state of Arizona; this serves as the implicated policy change. Data were collected via on-site survey administered February to May 2011. An overall score was created for the five motivational dimensions: 1 — Status; 2 — Economic; 3 — Refugee; 4 — Entrepreneurial; and, 5 — Political using principle component factor analysis using a varimax rotation with Kaiser normalization. Theory and literature suggest that the economic advancement, status advancement, and the refugee orientation are effective explanatory variables for motivating a career move into the tourism industry. A total of 82 questionnaires were delivered and completed (N = 82), and none were eliminated. The statistically-determined Economic Dimension was characterized by eleven statements explained 51% of the variation and was the overwhelming motivational force. The average coded response for change in job satisfaction was very positive at .75. Ten features of changes in job satisfaction were used as the basis of the second measure of change in job satisfaction. The first Principle Component of the ten features of job satisfaction change explained 45% of the variation in these features and loadings were positive near or above 0.60 for all items. The relationship between variations in each of the measurements of change in job satisfaction and motivating factors was explored using regression analysis. The two dependent variables were Overall Change and First Principle Component, and the independent variables for both regressions included the four motivating factors as measured by the rotated factors scores to represent dimensions of Economic, Status, Refugee and Entrepreneurial. In addition to the motivational factors, four demographic variables were included as independent variables to account for personal and situational differences. None of the regression coefficients were significant at even the 10% level. Although this result was expected, the positive sign of regression coefficients suggest that expectations of working as a housekeepers results in a positive outcome. Understanding this relationship further is necessary, and seeking larger sample sizes over a longer period of time would be most beneficial to this field of research.
ContributorsCasson, Mallory (Author) / Tyrrell, Timothy (Thesis advisor) / Budruk, Megha (Committee member) / Li, Wei (Committee member) / Arizona State University (Publisher)
Created2011
156116-Thumbnail Image.png
Description
Immunotherapy has been revitalized with the advent of immune checkpoint blockade

treatments, and neo-antigens are the targets of immune system in cancer patients who

respond to the treatments. The cancer vaccine field is focused on using neo-antigens from

unique point mutations of genomic sequence in the cancer patient for making

personalized cancer vaccines. However,

Immunotherapy has been revitalized with the advent of immune checkpoint blockade

treatments, and neo-antigens are the targets of immune system in cancer patients who

respond to the treatments. The cancer vaccine field is focused on using neo-antigens from

unique point mutations of genomic sequence in the cancer patient for making

personalized cancer vaccines. However, we choose a different path to find frameshift

neo-antigens at the mRNA level and develop broadly effective cancer vaccines based on

frameshift antigens.

In this dissertation, I have summarized and characterized all the potential frameshift

antigens from microsatellite regions in human, dog and mouse. A list of frameshift

antigens was validated by PCR in tumor samples and the mutation rate was calculated for

one candidate – SEC62. I develop a method to screen the antibody response against

frameshift antigens in human and dog cancer patients by using frameshift peptide arrays.

Frameshift antigens selected by positive antibody response in cancer patients or by MHC

predictions show protection in different mouse tumor models. A dog version of the

cancer vaccine based on frameshift antigens was developed and tested in a small safety

trial. The results demonstrate that the vaccine is safe and it can induce strong B and T cell

immune responses. Further, I built the human exon junction frameshift database which

includes all possible frameshift antigens from mis-splicing events in exon junctions, and I

develop a method to find potential frameshift antigens from large cancer

immunosignature dataset with these databases. In addition, I test the idea of ‘early cancer

diagnosis, early treatment’ in a transgenic mouse cancer model. The results show that

ii

early treatment gives significantly better protection than late treatment and the correct

time point for treatment is crucial to give the best clinical benefit. A model for early

treatment is developed with these results.

Frameshift neo-antigens from microsatellite regions and mis-splicing events are

abundant at mRNA level and they are better antigens than neo-antigens from point

mutations in the genomic sequences of cancer patients in terms of high immunogenicity,

low probability to cause autoimmune diseases and low cost to develop a broadly effective

vaccine. This dissertation demonstrates the feasibility of using frameshift antigens for

cancer vaccine development.
ContributorsZhang, Jian (Author) / Johnston, Stephen Albert (Thesis advisor) / Chang, Yung (Committee member) / Stafford, Phillip (Committee member) / Chen, Qiang (Committee member) / Arizona State University (Publisher)
Created2018
137289-Thumbnail Image.png
Description
This paper is based on a research undertaking to understand China's presence in Africa and how this relates to Western relations with the continent. The research attempts to determine which option, Europe/US or the Chinese, may be the most suitable partner in development for Africa, as well as discuss what

This paper is based on a research undertaking to understand China's presence in Africa and how this relates to Western relations with the continent. The research attempts to determine which option, Europe/US or the Chinese, may be the most suitable partner in development for Africa, as well as discuss what can be done to maximize the benefits, and mitigate the negative aspects of that relationship. A comparative analysis approach is used to judge the viability of each partner, and each is assessed according to a set of criteria, including the following: 1. Equitable and Respectful Relations 2. Maintenance of Sovereignty 3. Ability and Willingness to Finance Sustainable Development in Africa 4. Shared Experience and Understanding 5. Historical Element Drawing on the collected research presented in this document, the major finding is that the Chinese have a fundamentally different approach to aid and investment, and harbor conceptually distinctive ideas regarding development than the West. Based on the outcome of the comparative study against the above criteria, it is suggested that Africa may benefit from selecting China as a partner in achieving its own sustainable development, and suggestions are offered to effectively leverage this partnership.
ContributorsBoucher, Sara Alexandra (Author) / Aubrey, Lisa (Thesis director) / Li, Wei (Committee member) / Barrett, The Honors College (Contributor) / W. P. Carey School of Business (Contributor) / School of Social Transformation (Contributor)
Created2014-05
148048-Thumbnail Image.png
Description

This project analyzes the diversity of the various Chinese languages present in the Phoenix metropolitan area. The diversity and presence of these languages can be used to make inferences about different aspects of the Chinese American community in the Phoenix area, and therefore the dialects and compared to other aspects

This project analyzes the diversity of the various Chinese languages present in the Phoenix metropolitan area. The diversity and presence of these languages can be used to make inferences about different aspects of the Chinese American community in the Phoenix area, and therefore the dialects and compared to other aspects of the Chinese American immigration experience, such as where immigrants are from, what areas of Phoenix they reside, and the Chinese language skills of both the participants and their children. The data is then presented with historical context of the Phoenix Chinese community as well as a brief discussion on the current Chinese community in Phoenix as well as the acculturation of Chinese American children.

ContributorsMartin, Adam (Author) / Li, Wei (Thesis director) / Xie, Siqiao (Committee member) / Chemical Engineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
128353-Thumbnail Image.png
Description

Competing endogenous RNAs (ceRNAs) are RNA molecules that sequester shared microRNAs (miRNAs) thereby affecting the expression of other targets of the miRNAs. Whether genetic variants in ceRNA can affect its biological function and disease development is still an open question. Here we identified a large number of genetic variants that

Competing endogenous RNAs (ceRNAs) are RNA molecules that sequester shared microRNAs (miRNAs) thereby affecting the expression of other targets of the miRNAs. Whether genetic variants in ceRNA can affect its biological function and disease development is still an open question. Here we identified a large number of genetic variants that are associated with ceRNA's function using Geuvaids RNA-seq data for 462 individuals from the 1000 Genomes Project. We call these loci competing endogenous RNA expression quantitative trait loci or ‘cerQTL’, and found that a large number of them were unexplored in conventional eQTL mapping. We identified many cerQTLs that have undergone recent positive selection in different human populations, and showed that single nucleotide polymorphisms in gene 3΄UTRs at the miRNA seed binding regions can simultaneously regulate gene expression changes in both cis and trans by the ceRNA mechanism. We also discovered that cerQTLs are significantly enriched in traits/diseases associated variants reported from genome-wide association studies in the miRNA binding sites, suggesting that disease susceptibilities could be attributed to ceRNA regulation. Further in vitro functional experiments demonstrated that a cerQTL rs11540855 can regulate ceRNA function. These results provide a comprehensive catalog of functional non-coding regulatory variants that may be responsible for ceRNA crosstalk at the post-transcriptional level.

ContributorsLi, Mulin Jun (Author) / Zhang, Jian (Author) / Liang, Qian (Author) / Xuan, Chenghao (Author) / Wu, Jiexing (Author) / Jiang, Peng (Author) / Li, Wei (Author) / Zhu, Yun (Author) / Wang, Panwen (Author) / Fernandez, Daniel (Author) / Shen, Yujun (Author) / Chen, Yiwen (Author) / Kocher, Jean-Pierre A. (Author) / Yu, Ying (Author) / Sham, Pak Chung (Author) / Wang, Junwen (Author) / Liu, Jun S. (Author) / Liu, X. Shirley (Author) / College of Health Solutions (Contributor)
Created2017-05-02
128573-Thumbnail Image.png
Description

Two distinct monocyte (Mo)/macrophage (Mp) subsets (Ly6Clow and Ly6Chi) orchestrate cardiac recovery process following myocardial infarction (MI). Prostaglandin (PG) E2 is involved in the Mo/Mp-mediated inflammatory response, however, the role of its receptors in Mos/Mps in cardiac healing remains to be determined. Here we show that pharmacological inhibition or gene

Two distinct monocyte (Mo)/macrophage (Mp) subsets (Ly6Clow and Ly6Chi) orchestrate cardiac recovery process following myocardial infarction (MI). Prostaglandin (PG) E2 is involved in the Mo/Mp-mediated inflammatory response, however, the role of its receptors in Mos/Mps in cardiac healing remains to be determined. Here we show that pharmacological inhibition or gene ablation of the Ep3 receptor in mice suppresses accumulation of Ly6Clow Mos/Mps in infarcted hearts. Ep3 deletion in Mos/Mps markedly attenuates healing after MI by reducing neovascularization in peri-infarct zones. Ep3 deficiency diminishes CX3C chemokine receptor 1 (CX3CR1) expression and vascular endothelial growth factor (VEGF) secretion in Mos/Mps by suppressing TGFβ1 signaling and subsequently inhibits Ly6Clow Mos/Mps migration and angiogenesis. Targeted overexpression of Ep3 receptors in Mos/Mps improves wound healing by enhancing angiogenesis. Thus, the PGE2/Ep3 axis promotes cardiac healing after MI by activating reparative Ly6Clow Mos/Mps, indicating that Ep3 receptor activation may be a promising therapeutic target for acute MI.

ContributorsTang, Juan (Author) / Shen, Yujun (Author) / Chen, Guilin (Author) / Wan, Qiangyou (Author) / Wang, Kai (Author) / Zhang, Jian (Author) / Qin, Jing (Author) / Liu, Guizhu (Author) / Zuo, Shengkai (Author) / Tao, Bo (Author) / Yu, Yu (Author) / Wang, Junwen (Author) / Lazarus, Michael (Author) / Yu, Ying (Author) / College of Health Solutions (Contributor)
Created2017-03-03
168740-Thumbnail Image.png
Description
Since 2007-2008, Africa has become the center of estate-driven land acquisitions to produce feed, food, biofuels, and fiber for the international market, which has raised contested narratives of African development and critical sustainability challenges. This is the case for the “Sisal Belt” of Kilosa in Tanzania, where Chinese firms resurrected

Since 2007-2008, Africa has become the center of estate-driven land acquisitions to produce feed, food, biofuels, and fiber for the international market, which has raised contested narratives of African development and critical sustainability challenges. This is the case for the “Sisal Belt” of Kilosa in Tanzania, where Chinese firms resurrected former colonial sisal estates and generated wage labor for adjacent farmers, changing local agrarian structures with significant implications for smallholder cultivation and the environment at large. Land system science aims to understand the land use and land cover dynamics as a coupled social-environmental system, focusing on the spatiotemporal patterns and the underlying socioeconomic and environmental drivers, impacts, and feedbacks of land system change. Following this interest, this dissertation uses three empirical studies to understand the processes of land system transformation in the sisal belt region and examine the consequences of the co-development by the estate and smallholder agriculture. The first study conducts long-term time series land-cover mapping and remote sensing analysis via Google Earth Engine to detect land changes and their spatial and socio-economic linkages to estate operations and smallholder livelihoods. The second study applies agent-based modeling to assess the distinctions among smallholder households in land-use and livelihood decision-making mechanisms when confronting estate wage labor and cash crop opportunities. The third study quantifies and identifies critical tradeoffs between carbon, water services, and the outcomes of commodity economies based on distinct future scenarios of development visions and estate-smallholder relationships up to the year 2030. The findings of these studies advance the understanding of the human-environmental conditions of estate and smallholders in Tanzania under the African land rush underway, which is consistent with the interest of land system and sustainability sciences. The policy implications drawn from this dissertation suggest that the primary land users and decision-makers should recognize the history and realities of the existing agrarian systems and engage in creative ways that serve the estate and the smallholder, including improving smallholder production and localizing estate operations. Such policies should be informed by assessments of changes in the environment and its services and be guided by local knowledge, needs, and future aspirations.
ContributorsLi, Puyang (Author) / Li, Wei (Thesis advisor) / Turner II, B.L. (Thesis advisor) / Monson, Jamie (Committee member) / Ligmann-Zielinska, Arika (Committee member) / Arizona State University (Publisher)
Created2022
168396-Thumbnail Image.png
Description
This dissertation focuses on the psycho-cultural perceptions and social interactions among a sample of 58 Chinese immigrant women in the Maricopa County, the Phoenix metro area of, Arizona, and the manner in which they are able to negotiate multiple identity markers that in part influence and define their capacity to

This dissertation focuses on the psycho-cultural perceptions and social interactions among a sample of 58 Chinese immigrant women in the Maricopa County, the Phoenix metro area of, Arizona, and the manner in which they are able to negotiate multiple identity markers that in part influence and define their capacity to achieve and maintain self-referential growth. The central question this dissertation seeks to address is: what historical forms have emerged, accumulated and reproduced through the actions of women in spaces within and between households, networks and social relations, voluntary associations, political participation, economic and financial transactions, and educational, religious, and civic, recreational and artistic activities; and how are these symbolically represented?This research is comprised of three stages. First, I show how a group of Chinese immigrant women living in, Arizona, combine the Eastern and Western connotations of the Phoenix metro area, to create a fourfold conceptual metaphor of the phoenix. Second, I demonstrate that how such symbolization and metaphorization represent their personal immigration experience, femininity, ethnic identity, and geographic location. Third, I also highlight how they associate themselves with the heuristic of the phoenix as a tool for self-empowerment, virtue, well-being, and self-representation. This dissertation concludes that the Chinese women living in the Phoenix area not only apply the metaphor of the phoenix to themselves, but also reference this mythical bird in their social media ID, clubs names, and themed events, and include it in their oral traditions. In contrast, they reject, negotiate, or resist the stigma and stereotypes attached to the “dragon” symbol which often convey qualities of overpowering and irrational oppression in western mythology. Instead, they associate themselves with the heuristic of the phoenix as a tool for self-empowerment, virtue, well-being, and ethnic self-representation. Such metaphorization and symbolization contribute to their resistance to the symbolic violence by countering with their own powerful self-referential narratives, that have shaped their Chinese community.
ContributorsShi, Hua (Author) / Cruz-Torres, Maria (Thesis advisor) / Velez-Ibanez, Carlos (Committee member) / Li, Wei (Committee member) / Arizona State University (Publisher)
Created2021