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- Creators: School of Life Sciences
Sonoma County, CA is on an ambitious pathway to meeting stringent carbon emissions goals that are part of California Assembly Bill 32. At the county-level, climate planners are currently evaluating options to assist residents of the county in reducing their carbon footprint and also for saving money. The Sonoma County Energy Independence Program (SCEIP) is one such county-level measure that is currently underway. SCEIP is a revolving loan fund that eligible residents may utilize to install distributed solar energy on their property. The fund operates like a property tax assessment, except that it only remains for a period of 20 years rather than in perpetuity.
This analysis intends to estimate the potential countywide effect that the $100M SCEIP fund might achieve on the C02 and cost footprint for the residential building energy sector. A functional unit of one typical home in the county is selected for a 25 year analysis period. Outside source data for the lifecycle emissions generated by the production, installation and operations of a PV system are utilized. Recent home energy survey data for the region is also utilized to predict a “typical” system size and profile that might be funded by the SCEIP program. A marginal cost-benefit calculation is employed to determine what size solar system a typical resident might purchase, which drives the life cycle assessment of the functional unit. Next, the total number of homes that might be financed by the SCEIP bond is determined in order to forecast the potential totalized effect on the County’s lifecycle emissions and cost profile.
The final results are evaluated and it is determined that the analysis is likely conservative in its estimation of the effects of the SCEIP program. This is due to the fact that currently offered subsidies are not utilized in the marginal benefit calculation for the solar system but do exist, the efficiency of solar technology is increasing, and the cost of a system over its lifecycle is currently decreasing. The final results show that financing distributed solar energy systems using Sonoma County money is a viable option for helping to meet state mandated goals and should be further pursued.
To determine if the disruption of the MMR pathway results in the reduced conservation of methylated adenines as well as an increased tolerance for mutations that result in the loss or gain of new GATC sites, we surveyed individual clones isolated from experimentally evolving wild-type and MMR-deficient (mutL- ;conferring an 150x increase in mutation rate) populations of E. coli with whole-genome sequencing. Initial analysis revealed a lack of mutations affecting methylation sites (GATC tetranucleotides) in wild-type clones. However, the inherent low mutation rates conferred by the wild-type background render this result inconclusive, due to a lack of statistical power, and reveal a need for a more direct measure of changes in methylation status. Thus as a first step to comparative methylomics, we benchmarked four different methylation-calling pipelines on three biological replicates of the wildtype progenitor strain for our evolved populations.
While it is understood that these methylated sites play a role in the MMR pathway, it is not fully understood the full extent of their effect on the genome. Thus the goal of this thesis was to better understand the forces which maintain the genome, specifically concerning m6A within the GATC motif.