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This study focused on the connection between the EnvZ/OmpR two-component regulatory system and the iron homeostasis system in Escherichia coli, specifically how a mutant form of EnvZ11/OmpR is able to reduce the expression of fepA::lacZ, a reporter gene fusion in E. coli. FepA is one of several outer membrane siderophore

This study focused on the connection between the EnvZ/OmpR two-component regulatory system and the iron homeostasis system in Escherichia coli, specifically how a mutant form of EnvZ11/OmpR is able to reduce the expression of fepA::lacZ, a reporter gene fusion in E. coli. FepA is one of several outer membrane siderophore receptors that allow extracellular siderophores bound to iron to enter the cells to power various biological processes. Previous studies have shown that in E. coli cells that expressed a mutant allele of envZ, called envZ11, which led to altered expression of various iron genes including down regulation of fepA::lacZ. The wild type EnvZ/OmpR system is not considered to regulate iron genes, but because these envz11 strains had downregulated fepA::lacZ, this study was undertaken to understand the connection and mechanisms of this downregulation. A large number of Lac+ revertants were obtained from the B32-2483 strain (envz11 and fepA::lacZ) and 7 Lac+ revertants that had reversion mutations not directly correcting the envZ11 allele were further characterized. With P1 phage transduction genetic mapping that involved moving a kanamycin resistance marker linked to fepA::lacZ, two Lac+ revertants were found to have their reversion mutations in the fepA promoter region, while the other five revertants had their mutations mapping outside the fepA region. These two revertants underwent DNA sequencing and found to carry two different single base pair mutations in two different locations of the fepA promoter region. Each one is in the Fur repressor binding region, but one also may have affected the Shine-Dalgarno region involved in translation initiation. All 7 reveratants underwent beta-galactosidase assays to measure fepA::lacZ expression. The two revertants that had mutations in the fepA promoter region had significantly increased fepA activity, with the revertant with the Shine-Dalgarno mutation having the most elevated fepA expression. The other 5 revertants that did not map in the fepA region had fepA expression elevated to the same level as that found in the wild type EnvZ/OmpR background. The data suggest that the negative effect of envZ11 can be overcome by multiple mechanisms, including directly correcting the envZ11 allele or changing the fepA promoter region.
ContributorsKalinkin, Victor Arkady (Co-author) / Misra, Rajeev (Co-author, Thesis director) / Mason, Hugh (Committee member) / Foy, Joseph (Committee member) / Biomedical Informatics Program (Contributor) / School of Life Sciences (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
My passion on the importance of oral health began when I was four years old with the traumatic experience of witnessing my grandmother, my idol, with a toothless smile without her dentures in. At present, I have been a dental assistant for five years, and have heard of similar stories

My passion on the importance of oral health began when I was four years old with the traumatic experience of witnessing my grandmother, my idol, with a toothless smile without her dentures in. At present, I have been a dental assistant for five years, and have heard of similar stories of people struggling with maintaining oral hygiene and having access to oral health care, including: family members, friends, patients, coworkers, and even Arizona State University faculty. Since the abolishment of emergency dental services in Arizona's Health Care Cost Containment System, dental related oral health care rates have jumped incrementally. In addition Arizona has created a dental desert, because dentists are not setting up their practices in the rural areas of the Valley, due to wanting to generate the most amount of income. Studies have also shown that dentists feel inept at treating ethnically diverse patients as well as patients from lower socioeconomic classes. These problems create a myriad of mental effects and physical ailments on the patient that are not limited to the oral cavity. In this paper, these issues in oral health care were studied along with their effects, including recommendations for resolutions.
ContributorsTaylor, Janine Marie (Author) / Ingram-Waters, Mary (Thesis director) / Ili, Joan (Committee member) / School for the Future of Innovation in Society (Contributor) / School of Film, Dance and Theatre (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate adults who have been involved in prostitution. The aim of this paper was to test three pilot interventions to address stress experienced by the program

Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate adults who have been involved in prostitution. The aim of this paper was to test three pilot interventions to address stress experienced by the program clients through three different techniques which was given in a workshop format. Cognitive Behavior Therapy (CBT), Dance Movement Therapy (DMT), and yoga and meditation are the three types of stress reduction techniques that have been studied in previous research and were pilot tested with adult sex-trafficked victims. This particular sections discusses the benefits of DMT and the results obtained after teaching sex-trafficked victims this therapy. The results of the pilot studies found that all three techniques reduced levels of stress significantly, and that they want further testing.
ContributorsKhanal, Garima (Co-author) / Somayaji, Vallari (Co-author) / Satapathy, Nikita (Co-author) / Sepowitz, Dominique Roe (Thesis director) / Graff, Sarah (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate adults who have been involved in prostitution. The aim of this paper was to test three pilot interventions to address stress experienced by the program

Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate adults who have been involved in prostitution. The aim of this paper was to test three pilot interventions to address stress experienced by the program clients through three different techniques that were given in workshop format. Cognitive Behavior Therapy (CBT), Dance Movement Therapy, and yoga and meditation are the three types of stress reduction techniques that have been studied in previous research and were pilot tested with adult sex trafficking victims. The results of the pilot studies and all three techniques reduced levels of stress significantly, and they warrant future testing.
ContributorsSatapathy, Nikita (Co-author) / Khanal, Garima (Co-author) / Somayaji, Vallari (Co-author) / Roe-Sepowitz, Dominique (Thesis director) / Graff, Sarah (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate women who were involved in prostitution. The aim of this paper was to treat Post Traumatic Stress Disorder (PTSD) through three different techniques, which will

Sex trafficking is an issue that is prevalent in the United States, including in Arizona. The Catholic Charities Diversion Program in Phoenix seeks to rehabilitate women who were involved in prostitution. The aim of this paper was to treat Post Traumatic Stress Disorder (PTSD) through three different techniques, which will be given in the format of workshops. Cognitive Behavior Therapy (CBT), Dance Movement Therapy (DMT), and yoga and meditation are the three types of stress reduction techniques that have been studied in previous papers and will be used for sex-trafficked victims. It is important to combat stress and find stress reducing techniques to improve health and quality of life in an individual. This specific section of the study focuses on yoga and meditation therapy and how it helps to reduce stress. The results showed that all three techniques, including yoga and meditation, reduced levels of stress significantly, thereby giving them a tool to use on their own to improve their health, and that they can be further tested in a longitudinal study to examine the reduction of PTSD symptoms over time.
ContributorsSomayaji, Vallari (Co-author) / Khanal, Garima (Co-author) / Satapathy, Nikita (Co-author) / Roe-Sepowitz, Dominique (Thesis director) / Graff, Sarah (Committee member) / School of Life Sciences (Contributor) / School of Social Transformation (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
The Community Action Research Experiences (CARE) Program collaborated with a local non-profit organization called Arizona Facts of Life to design a program evaluation for their Youth Leadership Development Training Program, FACTS Curriculum. The purpose of this study was to identify targeted program outcomes, design an assessment to address these outcomes,

The Community Action Research Experiences (CARE) Program collaborated with a local non-profit organization called Arizona Facts of Life to design a program evaluation for their Youth Leadership Development Training Program, FACTS Curriculum. The purpose of this study was to identify targeted program outcomes, design an assessment to address these outcomes, and recommend possible evaluation designs. Arizona Facts of Life will implement the assessment using one of the recommended evaluation designs, and use the results to measure their Youth Leadership Development Training Program's outcomes and demonstrate efficacy.
Created2016-05
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Description
The purpose of this study is to analyze the stereotypes surrounding four wind instruments (flutes, oboes, clarinets, and saxophones), and the ways in which those stereotypes propagate through various levels of musical professionalism in Western culture. In order to determine what these stereotypes might entail, several thousand social media and

The purpose of this study is to analyze the stereotypes surrounding four wind instruments (flutes, oboes, clarinets, and saxophones), and the ways in which those stereotypes propagate through various levels of musical professionalism in Western culture. In order to determine what these stereotypes might entail, several thousand social media and blog posts were analyzed, and direct quotations detailing the perceived stereotypical personality profiles for each of the four instruments were collected. From these, the three most commonly mentioned characteristics were isolated for each of the instrument groups as follows: female gender, femininity, and giggliness for flutists, intelligence, studiousness, and demographics (specifically being an Asian male) for clarinetists, quirkiness, eccentricity, and being seen as a misfit for oboists, and overconfidence, attention-seeking behavior, and coolness for saxophonists. From these traits, a survey was drafted which asked participating college-aged musicians various multiple choice, opinion scale, and short-answer questions that gathered how much they agree or disagree with each trait describing the instrument from which it was derived. Their responses were then analyzed to determine how much correlation existed between the researched characteristics and the opinions of modern musicians. From these results, it was determined that 75% of the traits that were isolated for a particular instrument were, in fact, recognized as being true in the survey data, demonstrating that the stereotypes do exist and seem to be widely recognizable across many age groups, locations, and levels of musical skill. Further, 89% of participants admitted that the instrument they play has a certain stereotype associated with it, but only 38% of people identify with that profile. Overall, it was concluded that stereotypes, which are overwhelmingly negative and gendered by nature, are indeed propagated, but musicians do not appear to want to identify with them, and they reflect a more archaic and immature sense that does not correlate to the trends observed in modern, professional music.
ContributorsAllison, Lauren Nicole (Author) / Bhattacharjya, Nilanjana (Thesis director) / Ankeny, Casey (Committee member) / School of Life Sciences (Contributor) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Memory CD8+ T-cells can persist in the absence of antigen, primed for immediate activation and proliferation if later exposed to the same antigen. These cytotoxic lymphocytes provide long-term immunity following an acute infection. Studies have observed that intermediate levels of general T cell transfer prior to infection may cause an

Memory CD8+ T-cells can persist in the absence of antigen, primed for immediate activation and proliferation if later exposed to the same antigen. These cytotoxic lymphocytes provide long-term immunity following an acute infection. Studies have observed that intermediate levels of general T cell transfer prior to infection may cause an inappropriate response resulting in increased pathology rather than prevention. Therefore, our study focused on a memory CD8 T-cell therapy using lymphocytic choriomeningitis virus (LCMV) specific splenocytes, which activate and proliferate at an accelerated pace compared to that of naive T-cells. LCMV is a natural murine pathogen which also poses a zoonotic infection threat to humans, and the effect of immune cell vaccination therapies for LCMV is not fully understood. We observed the effect of multiple memory CD8 T cell dosage levels on overall disease and memory CD8 T-cell response to the virus. Infection by exposure to a carrier was shown to have a reduced impact on mice receiving higher doses of memory T cells prior to infection compared to mice receiving less or no memory cells. Higher presence of activated memory cells were shown to correlate with less disease-related weight loss and accelerated recovery times. Survival rate after exposure to carriers was not shown to be affected by dosage level, warranting further research regarding the prevalence of the immunopathology observed in other studies in natural murine transmission models.
ContributorsMiller, Charles (Author) / Blattman, Joseph (Thesis director) / Holechek, Susan (Committee member) / Carmen, Joshua (Committee member) / W. P. Carey School of Business (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
The stress response facilitates our ability to deal effectively with threatening situations, but exposure to severe or chronic stressors can lead to undesirable neural, physiological, and behavioral outcomes. Chronic stress is associated with structural changes in the rat hippocampus, with corresponding deficits in learning and memory. Recent studies have uncovered

The stress response facilitates our ability to deal effectively with threatening situations, but exposure to severe or chronic stressors can lead to undesirable neural, physiological, and behavioral outcomes. Chronic stress is associated with structural changes in the rat hippocampus, with corresponding deficits in learning and memory. Recent studies have uncovered an inherent neuroplasticity that allows the hippocampus to recover from these stress-induced neural changes. Underlying mechanisms likely involve several different cellular and molecular pathways. In order to gain a more comprehensive understanding of these pathways, we investigated differences in protein expression throughout the timeline of chronic stress and recovery. Male Sprague-Dawley rats were randomly assigned to chronic restraint stress for 6hr/d/10d or 6hr/d/21d, stress for 6hr/d/21d followed by a recovery period of no stress for 10 or 21 days, or a control group. The proteome from the hippocampus of these rats was sequenced using liquid chromatography tandem mass spectrometry (LC-MS/MS) and analyzed. We hypothesized that chronic stress alters interneuronal signaling in the hippocampus by enhancing or attenuating the expression of proteins responsible for synaptic plasticity (functional) and neuronal structure (morphology). So far we have found that structural proteins, such as alpha-internexin, homer protein homolog 3, neurofilament light, and vimentin were significantly altered by chronic stress and recovery. In contrast, proteins necessary for or associated with myelination such as 2',3'-cyclic-nucleotide 3'-phosphodiesterase, myelin-associated glycoprotein, myelin basic protein S, and myelin proteolipid protein were significantly downregulated by chronic stress. Collectively, these results will provide a resource for further investigations into the mechanisms of the brain's recovery from chronic stress.
ContributorsKachemov, Marketta Marilyn (Author) / Orchinik, Miles (Thesis director) / Pirrotte, Patrick (Committee member) / Conrad, Cheryl (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Historically, studies of condition-dependent signals in animals have been male-centric, but recent work suggests that female ornaments can also communicate individual quality (e.g., disease state, fecundity). There has been a surge of interest in how urbanization alters signaling traits, but we know little about if and how cities affect signal

Historically, studies of condition-dependent signals in animals have been male-centric, but recent work suggests that female ornaments can also communicate individual quality (e.g., disease state, fecundity). There has been a surge of interest in how urbanization alters signaling traits, but we know little about if and how cities affect signal expression in female animals. We measured carotenoid-based plumage coloration and coccidian (Isospora spp) parasite burden in desert and city populations of house finches to examine urban impacts on male and female health and attractiveness. In earlier work, we showed that male house finches are less colorful and more parasitized in the city, and we again detected that pattern in this study for males. However, though city females are also less colorful than their rural counterparts, we found that rural females were more parasitized. Also, regardless of sex and unlike rural birds, more colorful birds in the city were more heavily infected with coccidia. These results show that urban environments can disrupt signal honesty in female animals and highlight the need for more studies on how cities affect disease and condition-dependent traits in both male and female animals.
ContributorsSykes, Brooke Emma (Author) / McGraw, Kevin (Thesis director) / Sweazea, Karen (Committee member) / Hutton, Pierce (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05