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- Creators: Ira A. Fulton Schools of Engineering
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River rafting trips and hikers use sandbars along the Colorado River in Marble and Grand Canyons as campsites. The U.S. Geological Survey evaluated the effects of Glen Canyon Dam operations on campsite areas on sandbars along the Colorado River in Grand Canyon National Park. Campsite area was measured annually from 1998 to 2012 at 37 study sites between Lees Ferry and Diamond Creek, Arizona. The primary purpose of this report is to present the methods and results of the project.
Campsite area surveys were conducted using total station survey methods to outline the perimeter of camping area at each study site. Campsite area is defined as any region of smooth substrate (most commonly sand) with no more than an 8 degree slope and little or no vegetation. We used this definition, but relaxed the slope criteria to include steeper areas near boat mooring locations where campers typically establish their kitchens.
The results show that campsite area decreased over the course of the study period, but at a rate that varied by elevation zone and by survey period. Time-series plots show that from 1998 to 2012, high stage-elevation (greater than the 25,000 ft3/s stage-elevation) campsite area decreased significantly, although there was no significant trend in low stage-elevation (15,000–20,000 ft3/s) campsite area. High stage-elevation campsite area increased after the 2004 and 2008 high flows, but decreased in the intervals between high flows. Although no overall trend was detected for low stage-elevation campsite areas, they did increase after high-volume dam releases equal to or greater than about 20,000 ft3/s. We conclude that dam operations have not met the management objectives of the Glen Canyon Adaptive Management program to increase the size of camping beaches in critical and non-critical reaches of the Colorado River between Glen Canyon Dam and Lake Mead.
Summary:
Interview conducted by: Dr. Paul Hirt, Arizona southwestern U.S. state. State University and Jennifer Sweeney, Four East Historical Research, LLC. Glen Canyon Dam Adaptive Management Program Administrative History Project. Administered by Arizona southwestern U.S. state. State University Supported by a grant from the US Bureau of Reclamation.
Biography:
Paul Grams has worked directly with the Glen Canyon Dam Adaptive Management Program (GCDAMP) since 2008, as a program manager and research hydrologist at the Grand Canyon Monitoring and Research Center (GCMRC). His involvement in Grand Canyon studies goes back to 1991, when he took a Colorado River research trip as part of an undergraduate science course. Grams is an expert on the effects of dams on river geomorphology and sediment transport. He holds a BA in Geology from Middlebury College, an MS in Geology from Utah State University, and a PhD in Geography and Environmental Engineering from Johns Hopkins University.
Methods: The traditional methodology (Forced-Stare [FS]) measures TFBUT and IBI separately. TFBUT is measured under forced-stare conditions by an examiner using a stopwatch, while IBI is measured as the subject watches television. The new methodology (video capture manual analysis [VCMA]) involves retrospective analysis of video data of fluorescein-stained eyes taken through a slit lamp while the subject watches television, and provides TFBUT and BUA for each IBI during the 1-minute video under natural blink conditions. The FS and VCMA methods were directly compared in the same set of dry-eye subjects. The VCMA method was evaluated for the ability to discriminate between dry-eye subjects and normal subjects. The VCMA method was further evaluated in the dry eye subjects for the ability to detect a treatment effect before, and 10 minutes after, bilateral instillation of an artificial tear solution.
Results: Ten normal subjects and 17 dry-eye subjects were studied. In the dry-eye subjects, the two methods differed with respect to mean TFBUTs (5.82 seconds, FS; 3.98 seconds, VCMA; P = 0.002). The FS variables alone (TFBUT, IBI) were not able to successfully distinguish between the dry-eye and normal subjects, whereas the additional VCMA variables, both derived and observed (BUA, BUA/IBI, breakup rate), were able to successfully distinguish between the dry-eye and normal subjects in a statistically significant fashion. TFBUT (P = 0.034) and BUA/IBI (P = 0.001) were able to distinguish the treatment effect of artificial tears in dry-eye subjects.
Conclusion: The VCMA methodology provides a clinically relevant analysis of tear film stability measured in the context of a natural blink pattern.
Methods: Thirty-three dry eye subjects completed a single-center, single-visit, pilot CAE study. The primary endpoint was mean break-up area (MBA) as assessed by the OPI 2.0 system. Secondary endpoints included corneal fluorescein staining, tear film break-up time, and OPI 2.0 system measurements. Subjects were also asked to rate their ocular discomfort throughout the CAE. Dry eye endpoints were measured at baseline, immediately following a 90-minute CAE exposure, and again 30 minutes after exposure.
Results: The post-CAE measurements of MBA showed a statistically significant decrease from the baseline measurements. The decrease was relatively specific to those patients with moderate to severe dry eye, as measured by baseline MBA. Secondary endpoints including palpebral fissure size, corneal staining, and redness, also showed significant changes when pre- and post-CAE measurements were compared. A correlation analysis identified specific associations between MBA, blink rate, and palpebral fissure size. Comparison of MBA responses allowed us to identify subpopulations of subjects who exhibited different compensatory mechanisms in response to CAE challenge. Of note, none of the measures of tear film break-up time showed statistically significant changes or correlations in pre-, versus post-CAE measures.
Conclusion: This pilot study confirms that the tear film metric MBA can detect changes in the ocular surface induced by a CAE, and that these changes are correlated with other, established measures of dry eye disease. The observed decrease in MBA following CAE exposure demonstrates that compensatory mechanisms are initiated during the CAE exposure, and that this compensation may provide the means to identify and characterize clinically relevant subpopulations of dry eye patients.
Grading schemes for breast cancer diagnosis are predominantly based on pathologists' qualitative assessment of altered nuclear structure from 2D brightfield microscopy images. However, cells are three-dimensional (3D) objects with features that are inherently 3D and thus poorly characterized in 2D. Our goal is to quantitatively characterize nuclear structure in 3D, assess its variation with malignancy, and investigate whether such variation correlates with standard nuclear grading criteria.
Methodology
We applied micro-optical computed tomographic imaging and automated 3D nuclear morphometry to quantify and compare morphological variations between human cell lines derived from normal, benign fibrocystic or malignant breast epithelium. To reproduce the appearance and contrast in clinical cytopathology images, we stained cells with hematoxylin and eosin and obtained 3D images of 150 individual stained cells of each cell type at sub-micron, isotropic resolution. Applying volumetric image analyses, we computed 42 3D morphological and textural descriptors of cellular and nuclear structure.
Principal Findings
We observed four distinct nuclear shape categories, the predominant being a mushroom cap shape. Cell and nuclear volumes increased from normal to fibrocystic to metastatic type, but there was little difference in the volume ratio of nucleus to cytoplasm (N/C ratio) between the lines. Abnormal cell nuclei had more nucleoli, markedly higher density and clumpier chromatin organization compared to normal. Nuclei of non-tumorigenic, fibrocystic cells exhibited larger textural variations than metastatic cell nuclei. At p<0.0025 by ANOVA and Kruskal-Wallis tests, 90% of our computed descriptors statistically differentiated control from abnormal cell populations, but only 69% of these features statistically differentiated the fibrocystic from the metastatic cell populations.
Conclusions
Our results provide a new perspective on nuclear structure variations associated with malignancy and point to the value of automated quantitative 3D nuclear morphometry as an objective tool to enable development of sensitive and specific nuclear grade classification in breast cancer diagnosis.