Matching Items (13)
Description
Every minute and a half, an American is sexually assaulted (Department of Justice, 2017). After an instance of sexual assault, some victims are given the choice of having a sexual assault evidence kit (SAK) collected. These kits are designed to collect DNA evidence that will, in the best case scenario, result in the identification of the perpetrator. If the perpetrator cannot be located, the DNA profile can still be submitted to the FBI’s CODIS databank, which houses hundreds of thousands of DNA profiles from criminal cases, and may still lead to apprehension of the rapist. Unfortunately, some SAKs experience long delays, decades even, before being tested. To date, there are hundreds of thousands of untested SAKs that remain in police custody awaiting to be submitted for forensic profiling across the country. Here, we completed a holistic investigation of sexual assault response and SAK processing in Arizona. It is important to notice that the focus of our study not only includes SAK processing and the backlog but sexual assault prevention and improving victim reporting in an effort to understand the SAK “pipeline,” from assault to prosecution.
We identified problems in three major categories that negatively impact the SAK pipeline: historical inertia, legislative and institutional limitations, and community awareness. We found that a large number of SAKs in Arizona have remained untested due insufficient funding and staffing for public crime labs making it difficult for state labs to alleviate the SAK backlog while simultaneously responding to incoming cases (“Why the Backlog Exists,” n.d.). However, surveys of ASU undergraduate students revealed a significant interest in campus assault and the SAK backlog. Based on our findings, we suggest harnessing the interest of undergraduate students and recruiting them to specialized SAK-oriented forensic technician and sexual assault nurse examiner (SANE) training at ASU with the goal of creating a workforce that will alleviate the absence of trained professionals within the country. We also explore the possibility of the creation of a private crime laboratory at ASU devoted the processing of SAKs in Arizona as a measure of alleviating the demand on local public laboratories and providing a more economic alternative to commercial laboratories. The creation of an SAK laboratory at ASU would provide undergraduates the opportunity to learn more about real forensic analysis on campus, provide a pipeline for students to become technicians themselves, and help reduce and prevent a future SAK backlog in Arizona.
We identified problems in three major categories that negatively impact the SAK pipeline: historical inertia, legislative and institutional limitations, and community awareness. We found that a large number of SAKs in Arizona have remained untested due insufficient funding and staffing for public crime labs making it difficult for state labs to alleviate the SAK backlog while simultaneously responding to incoming cases (“Why the Backlog Exists,” n.d.). However, surveys of ASU undergraduate students revealed a significant interest in campus assault and the SAK backlog. Based on our findings, we suggest harnessing the interest of undergraduate students and recruiting them to specialized SAK-oriented forensic technician and sexual assault nurse examiner (SANE) training at ASU with the goal of creating a workforce that will alleviate the absence of trained professionals within the country. We also explore the possibility of the creation of a private crime laboratory at ASU devoted the processing of SAKs in Arizona as a measure of alleviating the demand on local public laboratories and providing a more economic alternative to commercial laboratories. The creation of an SAK laboratory at ASU would provide undergraduates the opportunity to learn more about real forensic analysis on campus, provide a pipeline for students to become technicians themselves, and help reduce and prevent a future SAK backlog in Arizona.
ContributorsStewart, Jamie (Co-author) / Brokaw, Danielle (Co-author) / Stone, Megan (Co-author) / Kanthaswamy, Sreetharan (Thesis director) / Oldt, Robert (Committee member) / School of Human Evolution & Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Due to the nature of animals, even domesticated pets, animal scavenging of human remains is an important taphonomic factor. This area of study has, however, been undercounted in the current literature. The purpose of this study was to begin the first step in creating a taphonomic profile for urban / household animal scavenging as distinguishable from manmade tool marks. Using volunteered animals and regularly available tools, alterations were made on beef ribs in order to characterize the distinguishing profiles between the two groups. It was found that animal scavenging alterations, in the short term (20 minutes used in this study) have a distinctly different appearance than tool mark alterations. Animal scavenging has less visible alterations, consistent bite morphology across different species, and symmetrical cut marks along the midsection of the long bones. Ultimately, this study was a successful first step in furthering taphonomic alteration database research across various biomes and conditions.
ContributorsLittle, Cody Lee (Author) / Kobojek, Kimberly (Thesis director) / Falsetti, Anthony (Committee member) / Ball, Becky (Committee member) / School of Human Evolution and Social Change (Contributor) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Forensic anthropologists rely on the validated sex determination methods that utilize post-cranial elements in adult remains, but, recently, research has been conducted to determine adult remain sex using just the skull. Similar research for sub-adult remains is lacking the robustness and validation that adult determination methods possess. This study utilized 20 crania measurements taken from CT scans of child patients with known sexes at the Phoenix Children’s Hospital to investigate if a sex determination method could be produced. The measurements were assessed with a t-Test, Linear Discriminant Analysis, and Principle Component Analysis to determine if sexual dimorphism was detectable and if the predictive model had discriminant power when the sample size was categorized by age. The results revealed that a few measurements were dimorphic, but were not statistically significant to determine the sex of a sub-adult outside of the sample population. Future investigations will remove age group classification to observe if this model can predict age.
ContributorsMani, Mary Rachel (Author) / Ferry, Lara (Thesis director) / Falsetti, Anthony (Committee member) / School of Mathematical and Natural Sciences (Contributor) / School of Human Evolution & Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
This study examines the utility of diagnostic CT scans as sources of data to quantify facial growth in children. Subadults often suffer facial trauma arising from a variety of circumstances. Pediatric surgeons are then confronted with devising surgical pre- and post-operative strategies that present numerous complications. One of these involves predicting how specific areas of a child's face will change with time. The proliferation of CT scans use in pre-operative planning throughout healthcare provides potential data for addressing many concerns, including those involving facial growth. To add to the burgeoning body of literature focusing on facial growth and provide insight anatomical variation this study used data derived from CT scans from Phoenix Children's Hospital. Quantitative data derived from CTs were used to examine normative growth and develop predictive equations that surgeons can use to visualize facial change for males and female patients.
ContributorsLee, Isaac Jet (Author) / Ferry, Lara (Thesis director) / Falsetti, Anthony (Committee member) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
Rhesus (Macaca mulatta) and cynomolgus (M. fascicularis) macaques are the most commonly used nonhuman primate models in biomedical research. It is therefore critical to correctly infer each study animal's ABO blood group phenotype to prevent fatal transfusion- and transplantation-induced immune responses. While most macaques can be efficiently and accurately phenotyped using a DNA-based assay, we have identified some animals that are unable to be classified as type A, B, or AB and therefore exhibit an indeterminate phenotype. The purpose of this study was to develop a protocol for resolving indeterminate blood group phenotypes and consequently determine if these animals do indeed belong to an O blood phenotype. We attempted both direct and cloning-based sequencing of 21 animals phenotyped as A, B, AB, or indeterminate in order to assess variation at the functional mutation site in exon 7 of the macaque ABO gene. Although direct-from-PCR Sanger sequencing was unable to generate reliable sequence results, our cloned plasmid protocol yielded high quality sequences consistent with known blood group-specific alleles and as such can be used to identify informative polymorphisms at this locus.
ContributorsVizor, Choice Popsira (Author) / Kanthaswamy, Sreetharan (Thesis director) / Oldt, Robert (Committee member) / Department of Information Systems (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
In 2009 the National Academy of Sciences issued a publication outlining current issues in forensic science as well as key recommendations to fix relevant problems in fields ranging from comparative forensics to the death investigation system itself. These relevant problems stem partially from a lack of a unification amongst nationwide standards and practices. A lack of resources, funding, and qualified personnel has halted any real change in death investigation.1 Rather than allow the disparate, varied, and sometimes less qualified role of the Coroner to persist in tandem with a Medical Examiner System, it is recommended that the United States suspend the Coroner System in its entirety to allow for a nationwide Medical Examiner System as the new sole standard in death investigation. This transition is both necessary and feasible. Presently, there are a number of challenges facing the proposed unification of the medicolegal death investigation field to include funding; addressing medical school needs; facilities in both rural and populated communities; and overarching legislative issues connected to such a large endeavor. This recommendation proposes solutions to establish a unified Medical Examiner System. This proposal is based on the state of New Mexico's death investigation system and thus can integrate forensic pathology into the medical education while utilizing the knowledge of practicing forensic pathologists where Medical Examiner Systems can fuse with medical schools. Milestones will be achieved in stages over a 15 year time frame as the United States makes the transition to a unified centralized Medical Examiner System.
ContributorsKrouzkevitch, Alexis Kayleigh Ann (Author) / Kobojek, Kimberly (Thesis director) / Falsetti, Anthony (Committee member) / School of Criminology and Criminal Justice (Contributor) / School of Mathematical and Natural Sciences (Contributor) / School of Human Evolution and Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Ancestry estimation in forensic anthropology has been one of the most complex determinations to make from the human skeleton. There is a long history in biological anthropology using different morphological characteristics from the skull and other areas of the skeleton but it remains a difficult estimation that always has some variability. Currently, more studies have been conducted in morphological and metric methods from the skull of ancestry estimation to better the accuracy of the determination. Since most forensic cases are not in the best condition, there also must be other estimation methods from other bones from the remains such as the cervical vertebrae and the femur. These methods have some degree of accuracy but are not as commonly used in forensic cases as the skull is. It seems that the best method for ancestry estimation is to use a combination of multiple methods, having multiple lines of evidence. With the advent of DNA, many researchers have started to study the use of DNA in ancestry estimation. Genetics can be used in ancestry estimation as certain populations have allele frequencies that can be quantified. Using ancestry informative markers (AIMS), DNA can be used to estimate the ancestry of an individual as well as the amount of admixture in the individual. Many different methods have been tested in genetic evaluation of ancestry and have been supported with good accuracy. However, DNA analysis is expensive and time consuming, putting more reliance on osteological methods. Social implications have had a tremendous impact on the fate of ancestry estimation in forensic anthropology. Anthropology has generally rejected the notion of races but it is still used in forensics due to how much it is inculcated into everyday society. Also, the overarching theme of admixture is becoming more prevalent in society. This causes the estimations in forensic anthropology to be extremely difficult. If more research into ancestry estimation does not continue, the determination will almost be impossible to be made.
ContributorsDowell, Kori Kalei (Author) / Falsetti, Anthony (Thesis director) / Kanthaswamy, Sreetharan (Committee member) / School of Human Evolution and Social Change (Contributor) / School of Mathematical and Natural Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
We used the Affymetrix® Genome-Wide Human SNP Array 6.0 to identify heterospecific markers and compare copy number and structural genomic variation between humans and rhesus macaques. Over 200,000 human copy number variation (CNV) probes were mapped to a Chinese and an Indian rhesus macaque sample. Observed genomic rearrangements and synteny were in agreement with the results of a previously published genomic comparison between humans and rhesus macaques. Comparisons between each of the two rhesus macaques and humans yielded 206 regions with copy numbers that differed by at least two fold in the Indian rhesus macaque and human, 32 in the Chinese rhesus macaque and human, and 147 in both rhesus macaques. The detailed genomic map and preliminary CNV data are useful for better understanding genetic variation in rhesus macaques, identifying derived changes in human CNVs that may have evolved by selection, and determining the suitability of rhesus macaques as human models for particular biomedical studies.
ContributorsNg, Jillian (Author) / Fass, Joseph N. (Author) / Durbin-Johnson, Blythe (Author) / Smith, David Glenn (Author) / Kanthaswamy, Sreetharan (Author) / New College of Interdisciplinary Arts and Sciences (Contributor)
Created2015-09-16
Description
Rhesus macaques (Macaca mulatta) are the most widely used nonhuman primate in biomedical research, have the largest natural geographic distribution of any nonhuman primate, and have been the focus of much evolutionary and behavioral investigation. Consequently, rhesus macaques are one of the most thoroughly studied nonhuman primate species. However, little is known about genome-wide genetic variation in this species. A detailed understanding of extant genomic variation among rhesus macaques has implications for the use of this species as a model for studies of human health and disease, as well as for evolutionary population genomics. Whole genome sequencing analysis of 133 rhesus macaques revealed >43.7 million single nucleotide variants, including thousands predicted to alter protein sequences, transcript splicing and transcription factor binding sites. Rhesus macaques exhibit 2.5-fold higher overall nucleotide diversity and slightly elevated putative functional variation compared with humans. This functional variation in macaques provides opportunities for analyses of coding and non-coding variation, and its cellular consequences. Despite modestly higher levels of non-synonymous variation in the macaques, the estimated distribution of fitness effects and the ratio of non-synonymous to synonymous variants suggest that purifying selection has had stronger effects in rhesus macaques than in humans. Demographic reconstructions indicate this species has experienced a consistently large but fluctuating population size. Overall, the results presented here provide new insights into the population genomics of nonhuman primates and expand genomic information directly relevant to primate models of human disease.
ContributorsXue, Cheng (Author) / Raveendran, Muthuswamy (Author) / Harris, R. Alan (Author) / Fawcett, Gloria L. (Author) / Liu, Xiaoming (Author) / White, Simon (Author) / Dahdouli, Mahmoud (Author) / Rio Deiros, David (Author) / Below, Jennifer E. (Author) / Salerno, William (Author) / Cox, Laura (Author) / Fan, Guoping (Author) / Ferguson, Betsy (Author) / Horvath, Julie (Author) / Johnson, Zach (Author) / Kanthaswamy, Sreetharan (Author) / Kubisch, H. Michael (Author) / Liu, Dahai (Author) / Platt, Michael (Author) / Smith, David G. (Author) / Sun, Binghua (Author) / Vallender, Eric J. (Author) / Wang, Feng (Author) / Wiseman, Roger W. (Author) / Chen, Rui (Author) / Muzny, Donna M. (Author) / Gibbs, Richard A. (Author) / Yu, Fuli (Author) / Rogers, Jeffrey (Author) / College of Liberal Arts and Sciences (Contributor)
Created2016-10-17
Description
Porbeagles (Lamna nasus) are a highly commercially important shark species that is threatened with extinction due to overfishing. Mitochondrial DNA (mtDNA) displacement loop (D-loop) sequence data from 18 Northwest (NW) Atlantic and 30 Southwest (SW) Pacific porbeagles reveal that these regional populations have been genetically separated between 1.39 and 1.25 million years ago (MYA), a time frame which correlates with the end of the earth’s last cooling period. There is far greater genetic differentiation (FST = 0.835) between the NW and SW populations than among sharks within each population supporting a very high level of divergence. A lack of gene flow probably stemming from their limited distribution to cold water temperatures (-1oC to 15oC) has led to their genetic divergence. The NW Atlantic population exhibited fewer haplotypes than the SW Pacific population (2 vs 4). The mean nucleotide diversity value of the NW Atlantic population was also 50% lower (0.00143 vs. 0.00228). Male and female NW Atlantic individuals reflected virtually identical mean population diversity values (0.00393 vs 0.00399); however, females were prevalent near shorelines while the males were more often found in open waters. Of the three age groups within the NW Atlantic population, the immature individuals exhibited the greatest mean nucleotide diversity (0.00452), followed by the sub-adult group (0.00293) and the mature group (0.00288), suggesting that dispersion starts earlier in their life cycle and reduces as they get older. The porbeagle population biology, as revealed by D-loop sequence information, may have significant implications for the conservation efforts of this species. As differences in age-based and sex-based dispersion exist, it is important to understand the relative contributions of gene flow by adults of both sexes in order to implement more effective conservation strategies.
ContributorsHickey, Kaitlyn (Author) / Kanthaswamy, Sreetharan (Thesis advisor) / Sulikowski, James (Committee member) / Zhao, Yunpeng (Committee member) / Arizona State University (Publisher)
Created2022