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- Creators: School of International Letters and Cultures
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Description
This study focused on the connection between the EnvZ/OmpR two-component regulatory system and the iron homeostasis system in Escherichia coli, specifically how a mutant form of EnvZ11/OmpR is able to reduce the expression of fepA::lacZ, a reporter gene fusion in E. coli. FepA is one of several outer membrane siderophore receptors that allow extracellular siderophores bound to iron to enter the cells to power various biological processes. Previous studies have shown that in E. coli cells that expressed a mutant allele of envZ, called envZ11, which led to altered expression of various iron genes including down regulation of fepA::lacZ. The wild type EnvZ/OmpR system is not considered to regulate iron genes, but because these envz11 strains had downregulated fepA::lacZ, this study was undertaken to understand the connection and mechanisms of this downregulation. A large number of Lac+ revertants were obtained from the B32-2483 strain (envz11 and fepA::lacZ) and 7 Lac+ revertants that had reversion mutations not directly correcting the envZ11 allele were further characterized. With P1 phage transduction genetic mapping that involved moving a kanamycin resistance marker linked to fepA::lacZ, two Lac+ revertants were found to have their reversion mutations in the fepA promoter region, while the other five revertants had their mutations mapping outside the fepA region. These two revertants underwent DNA sequencing and found to carry two different single base pair mutations in two different locations of the fepA promoter region. Each one is in the Fur repressor binding region, but one also may have affected the Shine-Dalgarno region involved in translation initiation. All 7 reveratants underwent beta-galactosidase assays to measure fepA::lacZ expression. The two revertants that had mutations in the fepA promoter region had significantly increased fepA activity, with the revertant with the Shine-Dalgarno mutation having the most elevated fepA expression. The other 5 revertants that did not map in the fepA region had fepA expression elevated to the same level as that found in the wild type EnvZ/OmpR background. The data suggest that the negative effect of envZ11 can be overcome by multiple mechanisms, including directly correcting the envZ11 allele or changing the fepA promoter region.
ContributorsKalinkin, Victor Arkady (Co-author) / Misra, Rajeev (Co-author, Thesis director) / Mason, Hugh (Committee member) / Foy, Joseph (Committee member) / Biomedical Informatics Program (Contributor) / School of Life Sciences (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This thesis explores the intersection of religion, social class, and politics during the late nineteenth century in Imperial Germany. Specifically, the focus of this work is on the Workers' Association of Saint Paul's in Aachen and Burtscheid, a Catholic working-class organization in the 1870s located in the city of Aachen, a rapidly industrializing city in the majority Catholic Prussian Rhineland. This organization was the largest Catholic working-class association in Germany in the 1870s, reaching 5,000 members by the middle of the decade, and also espoused the politics of Christian Socialism. This thesis explores the intersection of the possibly competing social identities of these workers between being Catholics and workers. To start, the scholarly framework of studying society and politics in Imperial Germany is discussed, especially the concept of rigidly constructed social milieus into five groups, two of them being the Catholics and the working-class, and how this work may suggest a challenge to this concept. Next, the background information of how a Catholic working-class came into existence, as it was the product of simultaneous nineteenth century processes of industrialization and a religious revival among German Catholics. The Kulturkampf was the force that politicized Catholicism in Germany, as the persecution of Catholic institutions by Prussia forced Catholics into a social and political "ghetto." Then, the Association of St. Paul's itself is discussed. First, the workers espoused their Catholic identity and religious solidarity during a time of persecution, but also emphasized the Christian basis for their brand of Socialism. This lent into their identity as part of the working-class. While they steadfastly rejected the "godlessness" of Social Democracy, the Christian Socialists also shared many similar social and political goals. This intersection between identities eventually led to political conflict in Aachen throughout the 1870s with the mainstream, bourgeois Catholics of the city. To conclude, the legacy of Christian Socialism on modern Germany is discussed, as well as its contribution to the complex politics of Imperial Germany.
ContributorsHarkness, John Hosea (Author) / Benkert, Volker (Thesis director) / Holian, Anna (Committee member) / School of Politics and Global Studies (Contributor) / School of International Letters and Cultures (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
For this thesis, the authors would like to create a hypothetical Private Equity Real Estate Investment firm that focuses on creating value for partners by taking an opportunistic approach to acquiring under-performing urban multi-family properties with large upside potential for investing. The project will focus on both the market analysis and financial modeling associated with investment strategy and transactions. There is a substantial amount of complexity within commercial real estate and this thesis seeks to offer an accurate and comprehensive documentary of the process, while simplifying it for everyday readers. Additionally, there are a significant amount of risk factors associated with investment decisions, so the best practices from the industry documented in this manuscript are valuable tools for successful investing in the future. To gain the most profound and reliable industry knowledge, the authors leveraged the experience of dozens of industry professionals through research and personal interviews. Through careful analysis, the authors were able to ascertain the current economic position in the real estate cycle and to create a plan for future investing. Additionally, they were able to identify and evaluate a specific asset for purchase. As a result, the authors found that multifamily properties are a sound investment for the next two years and that the company should slowly start to shift directions to office and retail in 2018.
ContributorsBacon, David (Co-author) / Soto, Justin (Co-author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Department of Finance (Contributor) / Department of Supply Chain Management (Contributor) / Department of Marketing (Contributor) / W. P. Carey School of Business (Contributor) / School of Accountancy (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The ability to draft and develop productive Major League players is vital to the success of any MLB organization. A core of cost-controlled, productive players is as important as ever with free agent salaries continuing to rise dramatically. In a sport where mere percentage points separate winners from losers at the end of a long season, any slight advantage in identifying talent is valuable. This study examines the 2004-2008 MLB Amateur Drafts in order to analyze whether certain types of prospects are more valuable selections than others. If organizations can better identify which draft prospects will more likely contribute at the Major League level in the future, they can more optimally spend their allotted signing bonus pool in order to acquire as much potential production as possible through the draft. Based on the data examined, during these five drafts high school prospects provided higher value than college prospects. While college players reached the Majors at a higher rate, high school players produced greater value in their first six seasons of service time. In the all-important first round of the draft, where signing bonuses are at their largest, college players proved the more valuable selection. When players were separated by position, position players held greater expected value than pitchers, with corner infielders leading the way as the position group with the highest expected value. College players were found to provide better value than high school players at defensively demanding positions such as catcher and middle infield, while high school players were more valuable among outfielders and pitchers.
ContributorsGildea, Adam Joseph (Author) / Eaton, John (Thesis director) / McIntosh, Daniel (Committee member) / Department of Economics (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Technology both stimulates and is simultaneously stimulated by people and society. As a classic example, games have shaped and have been shaped by people's preferences. Today, online computer games are roaming everywhere, attracting and connecting players all over the world. However, at the same time, a lesser form of technology has emerged alongside of online computer games. It is known as trading card games (TCGs). Surprisingly, TCGs have been able to compete on same level as online computer games. Looking at my past experiences, I offered a theory that encompasses three forms of social interactions to explain for TCGs' success. The three types of interactions are: interaction of identities, interaction of interests, and interaction of influences. Interaction of identities is the constant interchange of player information and knowledge through different mediums. Interaction of interests involves the exchange and quality of the tangible and intangible. Interaction of influences deals with the fluid flow of communication and ideas that players use to change an outcome, however consciously or unconsciously done. Not one of these three factors of interactions act along; each is a part of a grand picture of interaction of interactions. Altogether, these factors explain, at least in part, the current popularity of trading card games as a relatively simple technology in a society with a plethora of technologically advanced entertainments and games such as virtual computer gaming. A web-based survey was devised to further examine the effects of the three forms of interactions have on online computer game and trading card game players. The results were consistent with the premises of the three-factor interaction theory.
ContributorsQin, Sheng (Author) / Eaton, John P. (Thesis director) / Olsen, G. Douglas (Committee member) / Department of Psychology (Contributor) / Department of Management (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
In recent history, the world has been inspired to respond to the challenges faced by communities with ‘help’. This help has been administered with moderate success through community engagement strategies traditionally centered on social services provided through non-profit agencies. Social entrepreneurship has emerged in response to the lack of progress made in solving local and global issues with new innovations that have the potential to change the status quo and eliminate the problems for future generations. In social entrepreneurship, concerned individuals saw an opportunity to truly change the world. Higher education leaders have embraced social entrepreneurship, positioning university students as a driving force behind ideating creative and innovative solutions that can be implemented in communities to overcome a vast array of challenges from poverty to environmental sustainability. Despite the efforts of university staff and faculty, many student changemakers struggle to successfully implement their ideas and measure their impact. Factors such as how well the student understands the issue and community in addition to the extent to which the student is experienced in ideation, creative-problem solving, and implementation of projects contribute to the success or failure of a student social effort. Inspired by their experiences serving as director of Changemaker Central, the authors sought to understand the process of preparing students to be agents of change in the community. Having observed the variance in success among aspiring changemakers at Arizona State University (ASU), the researchers studied how to best support students in preparation for a high-impact career. The research analyzed students’ experiences in two of ASU’s social change programs, Changemaker Challenge (CC) and University Service-Learning (USL) and found a need for more cohesion between two programs and their represented methodologies in addition to a need for in-depth analysis on the student journey.
ContributorsMicevic, Vid (Co-author) / Fitzgerald, Kaitlyn (Co-author) / Henderson, Mark (Thesis director) / Smith, Jacqueline (Committee member) / Civil, Environmental and Sustainable Engineering Programs (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The loss of a loved one through suicide is a traumatic life event that brings about considerable emotional turmoil. In the present study, the term suicide loss survivor refers to an individual who is a family member or a friend of a person who died by suicide. Through the three chosen methods of gathering data, which are online surveys, in person interviews, and photography sessions, researchers highlight the personal experience of thirty-three suicide loss survivors. Supported by these various methods of data collection are the unique issues that accompany the bereavement of a suicide loss. The areas of focus are the emotional trauma, social stigma, and postvention resources utilized or made available to suicide loss survivors. Throughout interviews with suicide loss survivors, some of whom also identified as Arizona State University students, an additional opportunity for research emerged. Participants identified that Arizona State University is not effectively providing suicide awareness and prevention materials and training to its community, including staff and students. Recommendations for how Arizona State University can improve their current processes are discussed in the conclusion. By implementing the recommendations of prevention and postvention care, it is possible to educate students and staff and, in turn, allow Arizona State University to foster a culture of empathy for existing suicide loss survivors, while working on decreasing the risk of future suicides. This creative project and narrative analysis was performed by two individuals who themselves are suicide loss survivors and have taken their personal experiences as a foundation for the project's need.
ContributorsStockwell, Anna (Co-author) / Lashinske, Angela (Co-author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Department of Information Systems (Contributor) / Department of Supply Chain Management (Contributor) / Economics Program in CLAS (Contributor) / School of Accountancy (Contributor) / WPC Graduate Programs (Contributor) / W. P. Carey School of Business (Contributor) / School of Community Resources and Development (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Targeting Tumors: Inclusion of Functional Groups on Ion-Containing Block Copolymers to Combat Cancer
Description
This research attempts to determine the most effective method of synthesizing a peptide such that it can be utilized as a targeting moiety for polymeric micelles. Two melanoma-associated peptides with high in vitro and in vivo binding affinity for TNF receptors have been identified and synthesized. Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-ToF) was used to help verify the structure of both peptides, which were purified using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). The next steps in the research are to attach the peptides to a micelle and determine their impact on micelle stability.
ContributorsMoe, Anna Marguerite (Author) / Green, Matthew (Thesis director) / Jones, Anne (Committee member) / Sullivan, Millicent (Committee member) / Chemical Engineering Program (Contributor) / School of International Letters and Cultures (Contributor) / Sandra Day O'Connor College of Law (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This creative project consists of three short stories with a common theme of release, letting go, and exhalation. Nymphal Instar is a story about Tommy, a young boy, and his encounter with his uncle, a troubled man who has just returned from war. The story explores the idea of growth and maturation, and the ability to move past and let go of trauma. A Cat Goes Away is about a young man, Richard, who is required to simultaneously deal with the loss of his cat and the suicide attempts of his sister. He also runs into his sister's ex-husband and is forced to deal with him. The story explores the difficulty in recognizing one's own emotions and the importance of knowing the difference between what one can change and what one cannot. Since Diagnosis is a story about Kate, a woman who has just been diagnosed with cancer and who is unable to tell her loved ones. The story explores acceptance and the idea that letting go can allow one to live more fully. Though the three stories are disparate in their characters and events, they share a commonality in their endings and in the final realizations of the characters. There is a focus on the importance of breath and breathing, and the essentiality of acceptance and release.
ContributorsMyers, Alan Yutaka (Author) / McNally, T. M. (Thesis director) / Irish, Jenny (Committee member) / School of International Letters and Cultures (Contributor) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
DescriptionPanic at the Elbow: High School Baseball's Tommy John Surgery Epidemic reflects on the history of Tommy John surgery, examines the scale of the current epidemic, explores its underlying causes and ultimately recommends steps that parents and players can take to avoid such overuse injuries. Link to documentary: https://www.youtube.com/watch?v=31xvOCN_tqk
ContributorsMargiott, Benjamin James (Author) / Kurland, Brett (Thesis director) / Byck, Peter (Committee member) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05