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Simple symphony. II. Playful pizzicato

ContributorsBritten, Benjamin, 1913-1976 (Composer)

Characterization of Transgenic Arabidopsis thaliana Overexpressing a Type I H+ Pyrophosphatase and the Phloem Lipid-Associated Family Protein

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Overexpression of AVP1 (Arabidopsis vacuolar pyrophosphatase), a type I H+ pyrophosphatase, results in greater biomass, possibly due to a function in sucrose transport within the phloem. Overexpression of the phloem lipid-associated family protein (PLAFP) was shown to increase the number of vascular bundles in Arabidopsis. Could these two phenotypes complement…

Overexpression of AVP1 (Arabidopsis vacuolar pyrophosphatase), a type I H+ pyrophosphatase, results in greater biomass, possibly due to a function in sucrose transport within the phloem. Overexpression of the phloem lipid-associated family protein (PLAFP) was shown to increase the number of vascular bundles in Arabidopsis. Could these two phenotypes complement one another additively? In this work, double mutants overexpressing both AVP1 and PLAFP were characterized. These double mutants have enhanced biomass, greater leaf area, and a larger number of vascular bundles than the single mutant lines. Overexpression of PLAFP does not result in any increase in rhizosphere acidification capacity.

ContributorsWilson, Sean (Co-author) / Furstenau, Tara (Co-author) / Gaxiola, Roberto (Thesis director) / Mason, Hugh (Committee member) / Wojciechowski, Martin (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)

Created2014-05

Simple symphony. II. Playful pizzicato

Characterization of Transgenic Arabidopsis thaliana Overexpressing a Type I H+ Pyrophosphatase and the Phloem Lipid-Associated Family Protein

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