The goal of this study was to gain insights from adults on the autism spectrum regarding their recommendations for improving the accessibility of stores and restaurants. Four adults who indicated that they had a diagnosis of autism spectrum disorder or Asperger’s Syndrome volunteered to participate in a qualitative interview. The questions that participants were asked centered around their experiences in stores and restaurants, current accessibility guidelines, the impact of COVID-19 on their experiences, and their recommendations for increased accessibility. Recommendations fell into two main categories: changes to the environment and accommodations that could be provided. Participants suggested multiple ways to reduce their sensitivities to noise in stores and restaurants like lowering the music, creating a quiet hour, or providing noise cancelling headphones. Further efforts are needed to fully understand the issue of accessibility for autistic individuals.

This research investigates what aspects of certain college students' quarantine experience were contributing to specific changes in gender identity. For this project, a general survey was distributed, and multiple interviews were conducted with willing survey participants to gauge more in-depth information about this phenomenon. Through the survey portion of the research, I found that many Barrett students felt that their identity had changed over the course of the pandemic, and a unique subset of these students experienced a change in their gender identity. Interviews with these folks highlighted several mechanisms that fostered this phenomenon: first, that quarantine allowed them a time to introspect, second, that they were not being policed or scrutinized in public for their gender performance, and third, that this was taking place in a supportive physical and/or virtual environment. This new research provides insight into the specific experiences of nonbinary college students whose identity shifted over the course of the COVID-19 pandemic, exploring factors that can influence identity development. While this is a unique and niche situation, it illustrates changing trends in how younger generations view themselves and their gender identity.

For my thesis I chose to complete a creative project. This project was to start my very own vocal studio as a vocal instructor. I began by researching different business components, such as, policies, contracts, pricing, websites, etc. I then used this research to form my own policies, contracts and social pages. I also took a survey of vocal students at ASU, with IRB approval, that covered what they have liked or disliked bout past and urgent vocal lessons, any advice they had fr me, and different singing techniques that they found to be helpful. After completing this I recruited students and began teaching. Half way through teaching I surveyed my sunsets, with IRB approval to find out what they enjoyed or disliked about lessons and then repeated this survey at the end to see if improvement was made. I followed this up with a survey and a summary of everything learned and discussed.

Obesity has reached epidemic proportions all around the world, and it has doubled in prevalence in both adults and children in over 70 countries from 1980 to 2015 (Afshin et al., 2017). Excessive weight gain in this proportion has been shown to negatively affect human cognition, reward neurocircuitry, stress responsiveness, and quality of life (Morris et al., 2015). Obesity is an example of a complex interaction between the environment (i.e., high-fat diets) and heredity (i.e., polygenic patterns of inheritance). The overconsumption of a high-fat diet (HFD) is an environmental factor that commonly induces weight gain (Hariri & Thibault, 2010). Two dietary-induced phenotypes have been observed in rats as a bimodal distribution of weight gain: obesity-prone (OP) and obesity-resistant (OR). Levin et al. (1997) investigated male and female HFD-fed Sprague-Dawley rats designated as OR when their weight gains were less than the heaviest chow-fed controls, and OP when their weight gains were greater than the heaviest chow-fed controls. OP rats showed greater weight gain, similar energy intake (EI), and similar feed efficiency (FE) compared to OR rats. Pagliassotti et al. (1997) designated male HFD-fed Wistar rats as OP and OR based on upper and lower tertiles of weight gain. OP rats displayed greater weight gain and EI than OR rats. These investigations highlight a predicament regarding rodent research in obesity: independent variables such as rat age, gender, strain, distribution of dietary macronutrients, and fatty acid composition of HFD and chow vary considerably, making it challenging to generalize data. Our experiment utilized outbred male Sprague-Dawley rats (5-6 weeks) administered a chow diet (19% energy from fat; 3.1 kcal/g) and a lard-based HFD (60% energy from fat; 5.24 kcal/g) over eight weeks. Separate rat populations were examined over three consecutive years (2017-2020), and independent obesogenic environmental variables were controlled. We investigated the persistence of weight gain, EI, and FE in HFD-fed rats inclusive of a population of designated OP and OR rats based on tertiles of weight gain. We define persistence as being p > 0.05. We hypothesize that the profiles (periodic data) of the dependent variables (weight gain, EI, FE) will be similar and persistent throughout the three separate years, but the magnitudes (cumulative data) of the dependent variables will differ. Our findings demonstrate that HFD, OP, and OR groups were persistent for periodic and cumulative weight gain, along with FE across the three consecutive independent years. Our findings also demonstrate impersistence for periodic EI in all groups, along with impersistence in cumulative EI for CHOW, OP, and OR groups. Therefore, our results allude to an inconsistent relationship between EI and weight gain, indicating that EI does not completely explain weight gain. Thus, the weakness between EI and weight gain relationship may be attributed to a polygenic pattern of inheritance, possibly signaling a weight setpoint regardless of EI.

"Calico" takes a look at the Japanese art form of "Manga" and what makes it stand out from similar art forms seen in the west such as comic books and graphic novels. After researching art techniques and the history leading up to modern manga practices, "Calico" summarizes it all together in the form of a manga itself. The story of "Calico" uses manga art techniques to tell the story of a young girl who finds solace in a street cat following the death of her mother.

Migration allows animals to track favorable environments and avoid harmful conditions but is energetically costly. There are different types of migration, such as tidal/daily, seasonal, and lifetime. Locust migratory swarms are one such famous phenomena that can have dramatic effects on human livelihoods. During long-distance flight, locusts rely on lipid oxidation from fat stores, while initial flight is fueled by carbohydrates. However, limited studies have tested how dietary macronutrients affect insect flight performance. Therefore, we asked: How do different dietary macronutrient ratios affect prolonged flight migration? We predicted that high carbohydrate diets would lead to high body lipid synthesis which would increase flight performance. We reared locusts in three crowded cages from 5th instar to adulthood on artificial diet varying in p:c ratio, supplemented with lettuce and water tubes, ad libitum. We used 7-14-day old adult males for flight performance assays where each day we used new individuals for tethered flight for 12 h in wind tunnels (~12 km·h-1) and video recorded their flight. We found that locust flight duration and quality increased with a decrease of dietary p:c ratio. Using control groups of locusts, we estimated that across 1 day of flight (up to 12 h), locusts lost on average in all treatments ~25 or ~30% of their total body lipid content. We concluded that long distance flight is improved by a high carbohydrate and low protein diet for L. migratoria by increasing their fuel sources. This work was supported by NSF # 1942054.

Memory CD8+ T cells protect against secondary viral infections. They develop and maintain exclusively in circulation (e.g. central memory - Tcm) or are excluded from re-circulation (resident memory - Trm). The extracellular ATP receptor P2RX7 promotes both Tcm and Trm generation. High (P2RX7hi) P2RX7-expressing early effector cells show survival, memory and pluripotency genes. Conversely, many terminal effector (TE) and apoptosis genes are upregulated in low (P2RX7lo) P2RX7-expressing cells. Among these genes is the zinc-finger transcriptional repressor Zeb2, which promotes TE differentiation at the expense of the memory cell pool. Given that Zeb2 was higher in P2RX7lo early effector cells, we postulated that Zeb2 ablation would allow P2RX7-deficient CD8+ T cells to skew towards memory subsets. To test this, we used RNP-based CRISPR-Cas9 to knockout Zeb2 in wild type or P2RX7-deficient P14 cells. At the memory timepoint, Zeb2 ablation led to a rescue of the ability of P2RX7-deficient cells to differentiate into the CD62L+ Tcm and CD69hiCD103hi Trm subsets, as well as increase the population of each. Our data suggest that P2RX7 imprints a pro-memory signature that is, to some extent, dependent on the negative regulation of Zeb2.